Difference between revisions of "Team:ICT-Mumbai/Parts"
Revathireddy (Talk | contribs) |
Revathireddy (Talk | contribs) |
||
| Line 156: | Line 156: | ||
<br> | <br> | ||
| − | <p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2017/3/35/ICT_Mumbai_pro.png" height = " | + | <p style="text-align:center;"><img src="https://static.igem.org/mediawiki/2017/3/35/ICT_Mumbai_pro.png" height = "400%" width = "400%"></p> |
<br> | <br> | ||
| − | <p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The figure above depicts the genomic context of the <i>ychH</i> gene; the transcription start site is also indicated. Crp acts as a positive transcription regulator of <i>ychH</i>, which means that this gene is expressed when glucose concentrations are low (Ref. 3; <a href="">EcoCyc database</a>)</p> | + | <p style="height:70 px; font-family:'Lato'; font-size:20px; colour:lightgrey;">The figure above depicts the genomic context of the <i>ychH</i> gene; the transcription start site is also indicated. Crp acts as a positive transcription regulator of <i>ychH</i>, which means that this gene is expressed when glucose concentrations are low (Ref. 3; <a href="">EcoCyc database</a>).</p> |
<!--<div class="column full_size"> | <!--<div class="column full_size"> | ||
| Line 167: | Line 167: | ||
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code><groupparts></code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p> | <p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code><groupparts></code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p> | ||
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p> | <p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p> | ||
| − | |||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
<div class="column half_size"> | <div class="column half_size"> | ||
Revision as of 08:29, 1 November 2017
Our project involves metabolism of ammonia by Escherichia coli to produce a blue-coloured dye, indigoidine. We had to choose between using a constitutive and an inducible promoter to drive expression of the genes that we wish to express in E. coli.
Expression from inducible promoters requires addition of an inducing molecule. However, as it would be cumbersome and tedious to add the inducer to the device that will house the engineered E. coli, and as it would also contribute to the cost, we decided to use a constitutive promoter to drive gene expression.
We reasoned that the constitutive promoter of choice should have the following two properties: (1) it should not be a very strong promoter, so as to not lead to any toxicity to the cell, and (2) it should be active in low-nutrient conditions. Based on these two considerations, the commonly used glycolytic promoters were ruled out as possible choices.
The ychH promoter has been described in literature to be active under low glucose conditions (Ref. 1). Moreover, it is not a very strong promoter, compared to those frequently employed to express recombinant proteins in E. coli (Ref. 2). Therefore, the ychH promoter became our promoter of choice.
The figure above depicts the genomic context of the ychH gene; the transcription start site is also indicated. Crp acts as a positive transcription regulator of ychH, which means that this gene is expressed when glucose concentrations are low (Ref. 3; EcoCyc database).
Part Table
References:
1. Hollands K, Busby SJ, Lloyd GS (2007). New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome. FEMS Microbiol Lett 274:89-94. PMID: 17608696
2. Deb SS, Reshamwala SMS, Lali AM (2016). A series of template plasmids for Escherichia coli genome engineering. J Microbiol Methods 125:49-57. PMID: 27071533
