Difference between revisions of "Team:IONIS-PARIS"

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<h2>Project description :</h2>
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<p>
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Climatic disorders lead to the appearance of extreme temperature events, whether frost or dryness,
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negatively impacting crops production and the global agriculture sector economy.
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</br>
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In 2016, 60% of the French vineyard cultures were destroyed due to frosts. To solve this, we aim at creating
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a thermosensitive biological tool named Softer Shock, providing an agro-environmental remedy for farmers.
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The bacteria retained in our solution will specifically produce a biological protectant designed to
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prevent the dryness phenomena above 37°C and frost damages below 15°C. The duality of this “2 in 1”
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biological function finds its properties activated in response to meteorological events, and a capacity
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of definitive bacterial auto-destruction after activation. These biological qualities fall within the
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respect of French and European regulatory requirements towards their potential impact on biodiversity.
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</br>                                                                                       
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                                The underlying scientific principle is to engineer a thermo-inducible plasmid that will be transferred
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to a bacterium (E. coli). The objective is to make the bacterium produce specific compounds for both
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high and low temperatures. As a proof of concept, we chose the blue chromoprotein AmilCP as the compound
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to be expressed at low temperatures, and the red fluorescent protein eRFP as the compound to be expressed
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at hot temperatures. The cold and heat shock strategies will be based on two new parts allowing the
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specific expression of the two compounds at the desired temperature ranges. If this proof of concept
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works, we will be able to adapt our constructions to any compound of choice and apply it for plant protection.
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Revision as of 18:33, 20 October 2017

IONIS-PARIS

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