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| | <html> | | <html> |
| − | <div id="container">
| + | |
| − | <h1>InterLab</h1>
| + | |
| − | <div class="container_in"> | + | <div class="column full_size judges-will-not-evaluate"> |
| − | <div class="container_nav">
| + | <h3>★ ALERT! </h3> |
| − | <a href="#t1">Methods</a>
| + | <p>This page is used by the judges to evaluate your team for the <a href="https:// 2017.igem.org/ Judging/ Medals"> medal criterion</a> or <a href="https:// 2017.igem.org/ Judging/ Awards"> award listed above</a>. </p> |
| − | <span class="nav_2 t1C"><a href="#t1_1">Background</a><a href="#t1_2">Materials</a><a href="#t1_3">Protocol</a></span>
| + | <p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p> |
| − | <a href="#t2">Result</a>
| + | |
| − | <span class="nav_2 t2C"><a href="#t2_1">Sequencing</a><a href="#t2_2">Data</a></span>
| + | |
| − | </div>
| + | |
| − | <div class="container_content container_content_right">
| + | |
| − | <h2 id="t1">Methods</h2>
| + | |
| − | <h3 id="t1_1">Background</h3>
| + | |
| − | <div class="content">
| + | |
| − | <p> The interlab is aimed to create reliable and repeatable measurement data and standardize the measurement of fluorescence in labs around the world by testing replicability of data, thus achieving to support the engineering of biological experiment. | + | |
| − | This academic community prevents the disadvantages of difference in experiment by providing a detailed protocol and data analysis method.
| + | |
| − | Interlab has become one of the largest interlaboratory study worldwide in synthetic biology, hitherto.
| + | |
| − | The data collected from the previous studies is summarized in a presentation iGEM 2016 Interlab Results Presentation.
| + | |
| − | This time it is done by following the same protocols designed for a plate reader and a flow cytometer that will produce data in same units everywhere so it can be compared and analysed. Our team decided to test both protocols to measure the fluorescence.</p>
| + | |
| − | </div>
| + | |
| − | <h3 id="t1_2">Materials</h3>
| + | |
| − | <div class="content">
| + | |
| − | <h4>Plasmids used</h4>
| + | |
| − | <p>Positive Control Device: I20270 in pSB1C3 (Located in Kit Plate 6, well 20B)</p>
| + | |
| − | <p>Negative Control Device: R0040 in pSB1C3 (Located in Kit Plate 6, well 20D)</p>
| + | |
| − | <p>• Test Device 1: J23101+I13504 in pSB1C3 (Located in Kit Plate 6, well 20F)</p>
| + | |
| − | <p>• Test Device 2: J23106+I13504 in pSB1C3 (Located in Kit Plate 6, well 20H)</p>
| + | |
| − | <p>• Test Device 3: J23117+I13504 in pSB1C3 (Located in Kit Plate 6, well 20J)</p>
| + | |
| − | <p>• Test Device 4: J23101.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20L)</p>
| + | |
| − | <p>• Test Device 5: J23106.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20N)</p>
| + | |
| − | <p>• Test Device 6: J23117.BCD2.E0040.B0015 in pSB1C3 (Located in Kit Plate 6, well 20P)</p>
| + | |
| − | <h4>Strain used</h4>
| + | |
| − | <p>• Escherichia coli strain DH5α</p>
| + | |
| − | <h4>Materials</h4>
| + | |
| − | <p>• 1ml LUDOX</p>
| + | |
| − | <p>• H20</p>
| + | |
| − | <p>• 96 well plate</p>
| + | |
| − | <p>• fluorescein</p>
| + | |
| − | <p>• 10ml 1xPBS</p>
| + | |
| − | <p>• LB (Luria Bertani) media</p>
| + | |
| − | <p>• Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH - working stock 25 ug/mL)</p>
| + | |
| − | <p>• 50 ml Falcon tube</p>
| + | |
| − | <p>• Incubator at 37°C</p>
| + | |
| − | <p>• 1.5 ml eppendorf tubes</p>
| + | |
| − | <p>• Ice bucket with ice</p>
| + | |
| − | <p>• Pipettes</p>
| + | |
| − | <h4>Machines</h4>
| + | |
| − | <p>• Thermo Multiskan FC</p>
| + | |
| − | <p>• BERTHOLD TECHNOLOGIES LB940Mith</p>
| + | |
| − | </div>
| + | |
| − | <h3 id="t1_3">Protocol</h3>
| + | |
| − | <div class="content">
| + | |
| − | <a href="https:// static.igem.org/ mediawiki/ 2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf" target="_blank"> Calibration Protocols</a> | + | |
| − | <a href="https:// static.igem.org/ mediawiki/ 2017/8/85/InterLab_2017_Plate_Reader_Protocol.pdf" target="_blank"> Cell measurement protocol</a> | + | |
| − | </div>
| + | |
| − | <h2 id="t2">Result</h2>
| + | |
| − | <h3 id="t2_1">Sequencing</h3>
| + | |
| − | <div class="content">
| + | |
| − | <p>• Positive Control ：BBa_I20270</p>
| + | |
| − | <p>• Negative Control ：BBa_R0040</p>
| + | |
| − | <p>• Test Device 1 ：BBa_J364000</p>
| + | |
| − | <p>• Test Device 2 ：BBa_J364001</p>
| + | |
| − | <p>• Test Device 3 ：BBa_J364002</p>
| + | |
| − | <p>• Test Device 4 ：BBa_J364003</p>
| + | |
| − | <p>• Test Device 5 ：BBa_J364004</p>
| + | |
| − | <p>• Test Device 6 ：BBa_J364005</p>
| + | |
| − | <h4>> BBa_I20270 Part-only sequence (919 bp)</h4>
| + | |
| − | <p class="code">ttgatggctagctcagtcctaggtacaatgctagctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_R0040 Part-only sequence (54 bp)</h4>
| + | |
| − | <p class="code">tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac</p>
| + | |
| − | <h4>> BBa_J364000 Part-only sequence (918 bp)</h4>
| + | |
| − | <p class="code">tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_J364001 Part-only sequence (918 bp)</h4>
| + | |
| − | <p class="code">tttacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_J364002 Part-only sequence (918 bp)</h4>
| + | |
| − | <p class="code">ttgacagctagctcagtcctagggattgtgctagctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_J364003 Part-only sequence (990 bp)</h4>
| + | |
| − | <p class="code">tttacagctagctcagtcctaggtattatgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_J364004 Part-only sequence (990 bp)</h4>
| + | |
| − | <p class="code">tttacggctagctcagtcctaggtatagtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | <h4>> BBa_J364005 Part-only sequence (990 bp)</h4>
| + | |
| − | <p class="code">ttgacagctagctcagtcctagggattgtgctagctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata</p>
| + | |
| − | </div>
| + | |
| − | <h3 id="t2_2">Data</h3>
| + | |
| − | <div class="content">
| + | |
| − | <h4>OD600 Reference Point</h4>
| + | |
| − | <p>Table 1. OD600 Reference Point.</p>
| + | |
| − | <table>
| + | |
| − | <tr><th></th><th>LUDOX-HS40</th><th>H2O</th></tr>
| + | |
| − | <tr><td>Replicate 1</td><td>0.0497</td><td>0.0483</td></tr>
| + | |
| − | <tr><td>Replicate 2</td><td>0.0493</td><td>0.0463</td></tr>
| + | |
| − | <tr><td>Replicate 3</td><td>0.0518</td><td>0.0492</td></tr>
| + | |
| − | <tr><td>Replicate 4</td><td>0.0516</td><td>0.047</td></tr>
| + | |
| − | <tr><td>Arith. Mean</td><td>0.0506</td><td>0.0477</td></tr>
| + | |
| − | <tr><td>Corrected Abs600</td><td>0.0026667</td><td></td></tr>
| + | |
| − | <tr><td>Reference OD600</td><td>0.0425</td><td></td></tr>
| + | |
| − | <tr><td>OD600/Abs600</td><td>15.9375</td><td></td></tr>
| + | |
| − | </table>
| + | |
| − | <h4>Fluorescein Standard Curve</h4>
| + | |
| − | <p> Table 2. Data of Fluorescein Standard Curve.</ p> | + | |
| − | <table>
| + | |
| − | <tr><th>uM Fluorescein</th><th>50.00</th><th>25</th><th>12.5</th><th>6.25</th><th>3.125</th><th>1.5625</th><th>0.78125</th><th>0.390625</th><th>0.195313</th><th>0.097656</th><th>0.048828</th><th>0</th></tr>
| + | |
| − | <tr><td>Replicate 1</td><td>1937386</td><td>1040680</td><td>592161</td><td>322379</td><td>163819</td><td>86765</td><td>42958</td><td>22216</td><td>11739</td><td>5925</td><td>3079</td><td>444</td></tr>
| + | |
| − | <tr><td>Replicate 2</td><td>1859453</td><td>1115787</td><td>617759</td><td>316051</td><td>158096</td><td>82081</td><td>42161</td><td>21509</td><td>11052</td><td>5854</td><td>3048</td><td>444</td></tr>
| + | |
| − | <tr><td>Replicate 3</td><td>1874906</td><td>1099274</td><td>611612</td><td>327265</td><td>156925</td><td>77801</td><td>40314</td><td>19642</td><td>10255</td><td>5430</td><td>2655</td><td>373</td></tr>
| + | |
| − | <tr><td>Replicate 4</td><td>1893953</td><td>1082973</td><td>639359</td><td>340346</td><td>172348</td><td>86573</td><td>44775</td><td>21540</td><td>11446</td><td>6076</td><td>3351</td><td>414</td></tr>
| + | |
| − | <tr><td>Arith. Mean</td><td>1891425</td><td>1084679</td><td>615222.8</td><td>326510.3</td><td>162797</td><td>83305</td><td>42552</td><td>21226.75</td><td>11123</td><td>5821.25</td><td>3033.25</td><td>418.75</td></tr>
| + | |
| − | <tr><td>Arith. Std.Dev.</td><td>33733.71</td><td>32246.67</td><td>19441.13</td><td>10303.09</td><td>7043.812</td><td>4260.032</td><td>1850.053</td><td>1105.718</td><td>643.4931</td><td>276.7735</td><td>286.5593</td><td>33.61919</td></tr>
| + | |
| − | </table>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/b/b0/JNFLS-chart_1.png"/>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/b/b2/JNFLS-chart_2.png"/>
| + | |
| − | <h4>Normalization</h4>
| + | |
| − | <p>Table 3. Normalization.</p>
| + | |
| − | <table>
| + | |
| − | <tr><th>sample</th><th>Abs600 Reading</th><th>Volume of Preloading Culture</th><th>Volume of Preloading Media</th></tr>
| + | |
| − | <tr><td>positive control</td><td>0.090625</td><td>6.441223833</td><td>3.558776167</td></tr>
| + | |
| − | <tr><td>negative control</td><td>0.0985875</td><td>5.126561999</td><td>4.873438001</td></tr>
| + | |
| − | <tr><td>device 1</td><td>0.09785</td><td>5.225342913</td><td>4.774657087</td></tr>
| + | |
| − | <tr><td>device 2</td><td>0.10105</td><td>4.822182037</td><td>5.177817963</td></tr>
| + | |
| − | <tr><td>device 3</td><td>0.103175</td><td>4.587155963</td><td>5.412844037</td></tr>
| + | |
| − | <tr><td>device 4</td><td>0.0987375</td><td>5.106926269</td><td>4.893073731</td></tr>
| + | |
| − | <tr><td>device 5</td><td>0.1183625</td><td>3.402083776</td><td>6.597916224</td></tr>
| + | |
| − | <tr><td>device 6</td><td>0.0985</td><td>5.138086063</td><td>4.861913937</td></tr>
| + | |
| − | <tr><td>media+chl</td><td>0.059575</td><td></td><td></td></tr>
| + | |
| − | </table>
| + | |
| − | <h4>Cell Measurement</h4>
| + | |
| − | <p>Table 4. Raw data of Abs600 measurement.</p>
| + | |
| − | <table class="small">
| + | |
| − | <tr><th>Abs600 Raw Readings:</th><td colspan="2">Neg. Control</td><td colspan="2">Pos. Control</td><td colspan="2">Device 1</td><td colspan="2">Device 2</td><td colspan="2">Device 3</td><td colspan="2">Device 4</td><td colspan="2">Device 5</td><td colspan="2">Device 6</td><td colspan="2">LB + Chlor (blank)</td></tr>
| + | |
| − | <tr><th></th><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>0.09155</td><td>0.0897</td><td>0.090325</td><td>0.10685</td><td>0.104875</td><td>0.090825</td><td>0.09655</td><td>0.10555</td><td>0.1096</td><td>0.09675</td><td>0.107025</td><td>0.09045</td><td>0.13085</td><td>0.105875</td><td>0.1069</td><td>0.0901</td><td>0.0664</td><td>0.059575</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>0.1793</td><td>0.1855</td><td>0.10325</td><td>0.16325</td><td>0.10525</td><td>0.11365</td><td>0.17545</td><td>0.15035</td><td>0.202875</td><td>0.184825</td><td>0.1497</td><td>0.1019</td><td>0.19725</td><td>0.177475</td><td>0.1877</td><td>0.160825</td><td>0.0683</td><td>0.05675</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>0.2421</td><td>0.246575</td><td>0.134175</td><td>0.249425</td><td>0.117225</td><td>0.208075</td><td>0.23985</td><td>0.161875</td><td>0.4247</td><td>0.25865</td><td>0.230525</td><td>0.124025</td><td>0.25595</td><td>0.240925</td><td>0.244225</td><td>0.24665</td><td>0.06815</td><td>0.06885</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>0.3424</td><td>0.288625</td><td>0.208925</td><td>0.35995</td><td>0.133325</td><td>0.360225</td><td>0.37615</td><td>0.20765</td><td>0.58355</td><td>0.3692</td><td>0.303125</td><td>0.16655</td><td>0.36025</td><td>0.284425</td><td>0.291575</td><td>0.3055</td><td>0.0779</td><td>0.064775</td></tr>
| + | |
| − | </table>
| + | |
| − | <p>Table 5. Blank subtraction and correction of Abs600 measurement.</p>
| + | |
| − | <table class="medium">
| + | |
| − | <tr><th>OD600-Background</th><td colspan="2">Neg. Control</td><td colspan="2">Pos. Control</td><td colspan="2">Device 1</td><td colspan="2">Device 2</td><td colspan="2">Device 3</td><td colspan="2">Device 4</td><td colspan="2">Device 5</td><td colspan="2">Device 6</td></tr>
| + | |
| − | <tr><th></th><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>0.02515</td><td>0.0233</td><td>0.023925</td><td>0.04045</td><td>0.038475</td><td>0.024425</td><td>0.03015</td><td>0.03915</td><td>0.0432</td><td>0.03035</td><td>0.040625</td><td>0.02405</td><td>0.06445</td><td>0.039475</td><td>0.0405</td><td>0.0237</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>0.1129</td><td>0.1191</td><td>0.03685</td><td>0.09685</td><td>0.03885</td><td>0.04725</td><td>0.10905</td><td>0.08395</td><td>0.136475</td><td>0.118425</td><td>0.0833</td><td>0.0355</td><td>0.13085</td><td>0.111075</td><td>0.1213</td><td>0.094425</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>0.1757</td><td>0.180175</td><td>0.067775</td><td>0.183025</td><td>0.050825</td><td>0.141675</td><td>0.17345</td><td>0.095475</td><td>0.3583</td><td>0.19225</td><td>0.164125</td><td>0.057625</td><td>0.18955</td><td>0.174525</td><td>0.177825</td><td>0.18025</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>0.276</td><td>0.222225</td><td>0.142525</td><td>0.29355</td><td>0.066925</td><td>0.293825</td><td>0.30975</td><td>0.14125</td><td>0.51715</td><td>0.3028</td><td>0.236725</td><td>0.10015</td><td>0.29385</td><td>0.218025</td><td>0.225175</td><td>0.2391</td></tr>
| + | |
| − | </table>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/a/a9/JNFLS-chart_3.png"/>
| + | |
| − | <p>Figure 3. Blank subtraction and correction of Abs600 measurement.</p>
| + | |
| − | <p>Table 6. Raw data of fluorescence measurement.</p>
| + | |
| − | <table class="small">
| + | |
| − | <tr><th>Raw fluorescence</th><td colspan="2">Neg. Control</td><td colspan="2">Pos. Control</td><td colspan="2">Device 1</td><td colspan="2">Device 2</td><td colspan="2">Device 3</td><td colspan="2">Device 4</td><td colspan="2">Device 5</td><td colspan="2">Device 6</td><td colspan="2">LB + Chlor (blank)</td></tr>
| + | |
| − | <tr><th></th><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>11978.75</td><td>12054.25</td><td>13919.25</td><td>14055.25</td><td>12311.5</td><td>13868.5</td><td>17252.5</td><td>14736.75</td><td>12430.25</td><td>12286.5</td><td>14287.5</td><td>13227.5</td><td>12238.25</td><td>12178.25</td><td>11854.5</td><td>12076.75</td><td>11317.25</td><td>11138.25</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>12564</td><td>12755.75</td><td>16652</td><td>22236.5</td><td>15541.5</td><td>15458.5</td><td>24010.25</td><td>17610.75</td><td>13101.5</td><td>12591.75</td><td>20517.75</td><td>14232</td><td>14340.5</td><td>14186.5</td><td>12589.5</td><td>12354.75</td><td>10681.75</td><td>10787.5</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>13356.25</td><td>13479.75</td><td>21582.75</td><td>31126.25</td><td>13257.75</td><td>20684.25</td><td>28890.5</td><td>18862.25</td><td>14835</td><td>13921.5</td><td>28713.5</td><td>15357.25</td><td>15024.5</td><td>15387.5</td><td>13588.5</td><td>13468.25</td><td>11143.25</td><td>11640.5</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>14065.75</td><td>13709.75</td><td>27002.75</td><td>39536.5</td><td>14482</td><td>27169.5</td><td>38477</td><td>22481</td><td>17517.5</td><td>14774.75</td><td>32347.5</td><td>17138.75</td><td>16798.5</td><td>15973.25</td><td>14512</td><td>14396</td><td>11572.25</td><td>11271.75</td></tr>
| + | |
| − | </table>
| + | |
| − | <p>Table 7. Blank substraction and correction of fluorescence measurement.</p>
| + | |
| − | <table class="medium">
| + | |
| − | <tr><th>fluorescence-Background</th><td colspan="2">Neg. Control</td><td colspan="2">Pos. Control</td><td colspan="2">Device 1</td><td colspan="2">Device 2</td><td colspan="2">Device 3</td><td colspan="2">Device 4</td><td colspan="2">Device 5</td><td colspan="2">Device 6</td></tr>
| + | |
| − | <tr><th></th><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>661.5</td><td>737</td><td>2602</td><td>2738</td><td>994.25</td><td>2551.25</td><td>5935.25</td><td>3419.5</td><td>1113</td><td>969.25</td><td>2970.25</td><td>1910.25</td><td>921</td><td>861</td><td>537.25</td><td>759.5</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>1246.75</td><td>1438.5</td><td>5334.75</td><td>10919.25</td><td>4224.25</td><td>4141.25</td><td>12693</td><td>6293.5</td><td>1784.25</td><td>1274.5</td><td>9200.5</td><td>2914.75</td><td>3023.25</td><td>2869.25</td><td>1272.25</td><td>1037.5</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>2039</td><td>2162.5</td><td>10265.5</td><td>19809</td><td>1940.5</td><td>9367</td><td>17573.25</td><td>7545</td><td>3517.75</td><td>2604.25</td><td>17396.25</td><td>4040</td><td>3707.25</td><td>4070.25</td><td>2271.25</td><td>2151</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>2748.5</td><td>2392.5</td><td>15685.5</td><td>28219.25</td><td>3164.75</td><td>15852.25</td><td>27159.75</td><td>11163.75</td><td>6200.25</td><td>3457.5</td><td>21030.25</td><td>5821.5</td><td>5481.25</td><td>4656</td><td>3194.75</td><td>3078.75</td></tr>
| + | |
| − | </table class="medium">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/2/2e/JNFLS-chart_4.png"/>
| + | |
| − | <p>Figure 4. Blank subtraction and correction of fluorescence measurement.</p>
| + | |
| − | <p>Table 8.Raw data of Fl/Abs600.</p>
| + | |
| − | <table class="small">
| + | |
| − | <tr><th>FI/OD600</th><td colspan="2">Neg. Control</td><td colspan="2">Pos. Control</td><td colspan="2">Device 1</td><td colspan="2">Device 2</td><td colspan="2">Device 3</td><td colspan="2">Device 4</td><td colspan="2">Device 5</td><td colspan="2">Device 6</td></tr>
| + | |
| − | <tr><th></th><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td><td>Colony 1</td><td>Colony 2</td></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>0.0363656</td><td>0.0436894</td><td>0.1610903</td><td>0.1046872</td><td>0.0371617</td><td>0.2434395</td><td>0.3112388</td><td>0.1224077</td><td>0.0351709</td><td>0.0459574</td><td>0.1034099</td><td>0.1136499</td><td>0.0257567</td><td>0.0291739</td><td>0.0207388</td><td>0.0473831</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>0.015015</td><td>0.0165508</td><td>0.1993033</td><td>0.1555274</td><td>0.1629687</td><td>0.1344471</td><td>0.1612764</td><td>0.1036844</td><td>0.0178552</td><td>0.0149248</td><td>0.1559002</td><td>0.1161702</td><td>0.0335034</td><td>0.0354444</td><td>0.0141051</td><td>0.0152842</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>0.0159176</td><td>0.016459</td><td>0.2081384</td><td>0.1484984</td><td>0.0537376</td><td>0.0917684</td><td>0.1392104</td><td>0.1085667</td><td>0.0134581</td><td>0.018553</td><td>0.1457091</td><td>0.0972347</td><td>0.0276245</td><td>0.0320685</td><td>0.017551</td><td>0.0165957</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>0.0136599</td><td>0.014732</td><td>0.1650095</td><td>0.1317819</td><td>0.0655606</td><td>0.0739985</td><td>0.1257605</td><td>0.108465</td><td>0.0163216</td><td>0.016774</td><td>0.1236002</td><td>0.0795852</td><td>0.0257259</td><td>0.0296143</td><td>0.0193078</td><td>0.017636</td></tr>
| + | |
| − | </table>
| + | |
| − | <p>Table 9. Raw data of average</p>
| + | |
| − | <table>
| + | |
| − | <tr><th>Raw data of average</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>0.04002752</td><td>0.132888796</td><td>0.140300608</td><td>0.216823275</td><td>0.040564166</td><td>0.108529872</td><td>0.027465311</td><td>0.034060932</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>0.015782922</td><td>0.177415362</td><td>0.148707896</td><td>0.132480415</td><td>0.016390008</td><td>0.108529872</td><td>0.034473902</td><td>0.014694671</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>0.016188308</td><td>0.178318419</td><td>0.072753021</td><td>0.123888542</td><td>0.016005559</td><td>0.108529872</td><td>0.029846506</td><td>0.01707336</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>0.014195916</td><td>0.148395679</td><td>0.069779519</td><td>0.117112747</td><td>0.016547803</td><td>0.108529872</td><td>0.027670075</td><td>0.018471905</td></tr>
| + | |
| − | </table>
| + | |
| − | <p>Table 10. Raw data of SD</p>
| + | |
| − | <table>
| + | |
| − | <tr><th>Raw data of SD</th><th>Neg. Control</th><th>Pos. Control</th><th>Device 1</th><th>Device 2</th><th>Device 3</th><th>Device 4</th><th>Device 5</th><th>Device 6</th></tr>
| + | |
| − | <tr><th>Hour 0:</th><td>0.015079549</td><td>0.05186329</td><td>0.107849912</td><td>0.07821561</td><td>0.008373059</td><td>0.02479548</td><td>0.008997379</td><td>0.021020162</td></tr>
| + | |
| − | <tr><th>Hour 2:</th><td>0.002464455</td><td>0.011865598</td><td>0.047550627</td><td>0.014623325</td><td>0.00337995</td><td>0.029521958</td><td>0.005492679</td><td>0.003828995</td></tr>
| + | |
| − | <tr><th>Hour 4:</th><td>0.001357521</td><td>0.009555002</td><td>0.012588667</td><td>0.006161179</td><td>0.00202254</td><td>0.011469346</td><td>0.004159737</td><td>0.002278645</td></tr>
| + | |
| − | <tr><th>Hour 6:</th><td>0.002069122</td><td>0.029215462</td><td>0.005424471</td><td>0.012271926</td><td>0.003021193</td><td>0.00655286</td><td>0.003027256</td><td>0.002185333</td></tr>
| + | |
| − | </table>
| + | |
| − | <p>Figure 5. Average level of devices.</p>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2017/9/96/JNFLS-chart_5.png"/>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| | </div> | | </div> |
| | + | <div class="clear"></div> |
| | + | |
| | + | <div class="column full_size"> |
| | + | <h1>InterLab</h1> |
| | + | <h3>Bronze Medal Criterion #4</h3> |
| | + | <p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or improve the characterization of an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2017 part number range. |
| | + | <br><br> |
| | + | For teams participating in the <a href="https://2017.igem.org/Competition/InterLab_Study">InterLab study</a>, all work must be shown on this page. |
| | + | |
| | + | </p> |
| | </div> | | </div> |
| | + | |
| | + | |
| | + | |
| | + | |
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| | + | |
| | </html> | | </html> |
