Difference between revisions of "Team:JNFLS/Parts"

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<h1>Parts</h1>
 
<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<div class="top_text">We had devoted ourselves to explore a new method for treating colon cancer. It is well known that the oxygen partial pressure of solid tumor cells is very low, compared with normal cells. So we chose the hypoxia-induced promoter fdhF as the center part in our project.</div>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h2>1.Part Table</h2>
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<p>The parts we submitted are listed in the table below.</p>
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<tr><th>Part Number</th><th>Name</th><th>Type</th><th>Length</th><th>Description</th></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2358000">BBa_K2358000</a></td><td>fdhF promoter</td><td>Regulatory</td><td>99</td><td>Hypoxia-inducible promoter</td></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2358001">BBa_K2358001</a></td><td>fdhF inducible GFP device</td><td>Device</td><td>984</td><td>fdhF promoter controling GFP expression device</td></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2358002">BBa_K2358002</a></td><td>TAT</td><td>Coding</td><td>309</td><td>Trans-Activator of Transcription</td></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2358004">BBa_K2358004</a></td><td>hTERT</td><td>Regulatory</td><td>765</td><td>tumor-specific promoter</td></tr>
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<tr><td><a href="http://parts.igem.org/Part:BBa_K2358005">BBa_K2358005</a></td><td>survivin</td><td>Regulatory</td><td>980</td><td>tumor-specific promoter</td></tr>
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</table>
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<em style="text-align:center;">Table 1 parts information we submitted.</em>
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<h2>2.Functions of the Parts</h2>
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<p>Both BBa_K2358000 and BBa_K2358001 (composed of BBa_K2358000 and BBa_E0840) are related with hypoxia, and we detected the start efficiency of the hypoxia inducible promoter. With the culture time passed, oxygen partial pressure decreased, and down to below zero quickly. Since the instrument can not detect the value below zero, we used the increasing of CO2 partial pressure to represent the decreasing of O2 partial pressure in the experiment.</p>
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<img src="https://static.igem.org/mediawiki/2017/3/3f/JNFLS-Parts-2.png" />
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<img class="medium" src="https://static.igem.org/mediawiki/2017/8/8b/JNFLS-Parts-3.png" />
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<p>BBa_K2358002 is a regulatory protein that drastically enhances the efficiency of viral transcription. The protein is released by infected cells in culture, and is found in the blood of HIV-1 infected patients. It also can be absorbed by cells that are not infected with HIV, and can act directly as a toxin producing cell death via apoptosis in uninfected bystander cells. For more details, please refer to the <a href="https://2017.igem.org/Team:JNFLS/Measurement">results</a>.</p>
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<p>BBa_K2358004 and BBa_K2358005 are tumor-specific promoters. They can only start gene expression in tumor and embryonic tissues. It is closely related to the proliferation and infiltration of tumor cells.</p>
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<h5>Note</h5>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
 
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<h5>Adding parts to the registry</h5>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<h5>What information do I need to start putting my parts on the Registry?</h5>
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<p>The information needed to initially create a part on the Registry is:</p>
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<li>Part Name</li>
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h5>Inspiration</h5>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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<h5>Part Table </h5>
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
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<groupparts>iGEM17 JNFLS</groupparts>
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Latest revision as of 20:30, 1 March 2018

HOME
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JUDGING FORM

JNFLS

Parts

Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <groupparts> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.

Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.

Note

Note that parts must be documented on the Registry. This page serves to showcase the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.

Adding parts to the registry

You can add parts to the Registry at our Add a Part to the Registry link.

We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you do need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)

What information do I need to start putting my parts on the Registry?

The information needed to initially create a part on the Registry is:

  • Part Name
  • Part type
  • Creator
  • Sequence
  • Short Description (60 characters on what the DNA does)
  • Long Description (Longer description of what the DNA does)
  • Design considerations

We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page.

Inspiration

We have a created a collection of well documented parts that can help you get started.

You can also take a look at how other teams have documented their parts in their wiki:

Part Table

Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.

<groupparts>iGEM17 JNFLS</groupparts>