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     <h1>InterLab</h1>

     <h1>InterLab</h1>

     <h2>Introduction</h2>

     <h2 id="Introduction-0">Introduction</h2>

     <p>As a bronze medal requirement, iGEM teams have to partake in the InterLab study. These experiments carried out by laboratories across the world ensure that their studies are reliable and repeatable, a key component of scientific disciplines.

     <p>As a bronze medal requirement, iGEM teams have to partake in the InterLab study. These experiments carried out by laboratories across the world ensure that their studies are reliable and repeatable, a key component of scientific disciplines.

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     <h2>Experiments</h2>

     <h2 id="Experiments-1">Experiments</h2>

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Note: Due to being a high school team we did not own a plate reader so our InterLab study was carried out at the University of Kent in conjunction with their iGEM team’s InterLab study.</p>  

Note: Due to being a high school team we did not own a plate reader so our InterLab study was carried out at the University of Kent in conjunction with their iGEM team’s InterLab study.</p>  

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OD600 Reference Point: This was carried out in order calculate a conversion ratio from our absorbance data (measured by LUDOX-S40) to a standard OD600  (optical density) measurement.</p>

OD600 Reference Point: This was carried out in order calculate a conversion ratio from our absorbance data (measured by LUDOX-S40) to a standard OD600  (optical density) measurement.</p>

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     <p>Plate reader Settings:</p>   

Plate reader Settings:</p>   

<p>&nbsp;- Wavelength: 600nm</p>  

<p>Wavelength: 600nm</p>  

<p>&nbsp;- Path Length Correction: Off </p>

<p>Path Length Correction: Off </p>

   

   

<p>Fluorescein Fluorescence Standard Curve: This was done to measure the fluorescence of varying fluorescein concentrations produced from a serial dilution. A standard curve was then produced so that cell based fluorescence readings could be converted to a fluorescein concentration and therefore a GFP concentration. </p>

<p>Fluorescein Fluorescence Standard Curve: This was done to measure the fluorescence of varying fluorescein concentrations produced from a serial dilution. A standard curve was then produced so that cell based fluorescence readings could be converted to a fluorescein concentration and therefore a GFP concentration. </p>