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− | <div> | + | <div><div><div><div> |
| <h1>InterLab</h1> | | <h1>InterLab</h1> |
− | <h2>Introduction</h2> | + | <h2 id="Introduction-0">Introduction</h2> |
| <p>As a bronze medal requirement, iGEM teams have to partake in the InterLab study. These experiments carried out by laboratories across the world ensure that their studies are reliable and repeatable, a key component of scientific disciplines. | | <p>As a bronze medal requirement, iGEM teams have to partake in the InterLab study. These experiments carried out by laboratories across the world ensure that their studies are reliable and repeatable, a key component of scientific disciplines. |
| </p> | | </p> |
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| </p> | | </p> |
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− | <h2>Experiments</h2> | + | <h2 id="Experiments-1">Experiments</h2> |
| <p> | | <p> |
| Note: Due to being a high school team we did not own a plate reader so our InterLab study was carried out at the University of Kent in conjunction with their iGEM team’s InterLab study.</p> | | Note: Due to being a high school team we did not own a plate reader so our InterLab study was carried out at the University of Kent in conjunction with their iGEM team’s InterLab study.</p> |
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| OD600 Reference Point: This was carried out in order calculate a conversion ratio from our absorbance data (measured by LUDOX-S40) to a standard OD600 (optical density) measurement.</p> | | OD600 Reference Point: This was carried out in order calculate a conversion ratio from our absorbance data (measured by LUDOX-S40) to a standard OD600 (optical density) measurement.</p> |
− | <p> | + | <p>Plate reader Settings:</p> |
− | Plate reader Settings:</p> | + | <p> - Wavelength: 600nm</p> |
− | <p>Wavelength: 600nm</p> | + | <p> - Path Length Correction: Off </p> |
− | <p>Path Length Correction: Off </p> | + | |
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| <p>Fluorescein Fluorescence Standard Curve: This was done to measure the fluorescence of varying fluorescein concentrations produced from a serial dilution. A standard curve was then produced so that cell based fluorescence readings could be converted to a fluorescein concentration and therefore a GFP concentration. </p> | | <p>Fluorescein Fluorescence Standard Curve: This was done to measure the fluorescence of varying fluorescein concentrations produced from a serial dilution. A standard curve was then produced so that cell based fluorescence readings could be converted to a fluorescein concentration and therefore a GFP concentration. </p> |
| <p>Plate reader Settings:</p> | | <p>Plate reader Settings:</p> |
− | <p>Path Length Correction: Off</p> | + | <p> - Path Length Correction: Off</p> |
− | <p>Number of Flashes per Well: 22 </p> | + | <p> - Number of Flashes per Well: 22 </p> |
− | <p>Orbital Averaging: 600nm </p> | + | <p> - Orbital Averaging: 600nm </p> |
− | <p>Emission Wavelength: 520nm</p> | + | <p> - Emission Wavelength: 520nm</p> |
− | <p>Excitation Wavelength: 485nm </p> | + | <p> - Excitation Wavelength: 485nm </p> |
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| <p>Cell Measurement: This was done to look at the fluorescence of the different test devices over time. It involved taking regular readings of the Abs600 and the fluorescence. </p> | | <p>Cell Measurement: This was done to look at the fluorescence of the different test devices over time. It involved taking regular readings of the Abs600 and the fluorescence. </p> |
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| <p>Plate Reader Settings:</p> | | <p>Plate Reader Settings:</p> |
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