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Line 393: |
| <img src="https://static.igem.org/mediawiki/2017/8/84/T--Lambert_GA--laci.png"> | | <img src="https://static.igem.org/mediawiki/2017/8/84/T--Lambert_GA--laci.png"> |
| <br></center> | | <br></center> |
− | This part is a part of a larger construct which allows the system to change from one state to another. When the <i>E. coli</i>
| + | stufffffff about tspurple |
− | cells containing the larger construct is induced with IPTG, the IPTG binds with the LacI repressor, allowing the other pathway to be expressed, and it expresses a CI repressor which shuts down the first pathway. This part was created in order for teams to be able to easily place their genes of interest in this "switch" system. They would combine their gene of interest with the pLamdaR-LacI part and then proceed to combine it to the rest of the system. We had to make sure there were no illegal restriction enzyme sites (EcoRI, PstI, SpeI, and XbaI) which cut the part in places where we did not want them to. The part was created by combining multiple parts found on the iGEM parts registry.
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| <br><br><br> | | <br><br><br> |
| <center><font color= "#D49AE6">BBa_K2353001: tsPurpleDAS </font> | | <center><font color= "#D49AE6">BBa_K2353001: tsPurpleDAS </font> |