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<a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a> | <a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
− | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection"> | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a> |
<a class="drplink" style="transition: color 0.5s ease-in-out;" | <a class="drplink" style="transition: color 0.5s ease-in-out;" | ||
href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a> | href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a> | ||
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--><a href="https://2017.igem.org/Team:Lambert_GA/Safety"class="dropbtn">Safety</a><!-- | --><a href="https://2017.igem.org/Team:Lambert_GA/Safety"class="dropbtn">Safety</a><!-- | ||
--><li class="dropdown" > | --><li class="dropdown" > | ||
− | <a href="https://2017.igem.org/Team:Lambert_GA/ | + | <a href="https://2017.igem.org/Team:Lambert_GA/HP/Silver" class="dropbtn">Human Practices</a> |
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | ||
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | ||
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a> | ||
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a> | ||
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<br> | <br> | ||
− | <center><font color= "#D49AE6">BBa_K2353000: tsPurple </font> | + | <center><font color= "#D49AE6"><a href="http://parts.igem.org/Part:BBa_K2353000" style="text-decoration: none;"> BBa_K2353000: tsPurple</a> </font> |
<br> | <br> | ||
<img src="https://static.igem.org/mediawiki/2017/4/4f/TsPurple.png"><br></center> | <img src="https://static.igem.org/mediawiki/2017/4/4f/TsPurple.png"><br></center> | ||
− | + | <br><br> | |
− | + | <center><font color= "#D49AE6"><a href="http://parts.igem.org/Part:BBa_K2353001" style="text-decoration: none;">BBa_K2353001: tsPurpleDAS</a> </font> | |
− | <center><font color= "#D49AE6">BBa_K2353001: tsPurpleDAS </font> | + | |
<br><img src="https://static.igem.org/mediawiki/2017/a/a7/TsPurpleDAS.png"><br></center> | <br><img src="https://static.igem.org/mediawiki/2017/a/a7/TsPurpleDAS.png"><br></center> | ||
− | + | <br><br> | |
− | + | <center><font color= "#D49AE6"><a href="http://parts.igem.org/Part:BBa_K2353002" style="text-decoration: none;">BBa_K2353002: tsPurpleLAA</a> </font> | |
− | <center><font color= "#D49AE6">BBa_K2353002: tsPurpleLAA </font> | + | |
<br><img src="https://static.igem.org/mediawiki/2017/a/a2/TsPurpleLAA.png"><br></center> | <br><img src="https://static.igem.org/mediawiki/2017/a/a2/TsPurpleLAA.png"><br></center> | ||
− | + | <br> | |
+ | <p style="font-size:20px; text-indent: 50px; color: white;">The middle part of the full three part construct is one of three tsPurple sequences. Each sequence is composed of BBa_B0034 (RBS) and BBa_K1033906 (tsPurple- a purple chromoprotein also known as TinselPurple). When assembled with p-lambda-r LacI and pLac-ClpXP-CI, the tsPurple constructs are expressed by p-lambda-r LacI. The terminator for tsPurple is located at the beginning of the pLac-ClpXP-CI sequence, before the promoter for ClpXP-CI. When the construct is fully assembled and induced with IPTG, ClpXP is expressed and causes degradation when there is a degradation tag attached at the end of the tsPurple construct. BBa_K2353000, the tsPurple construct without any degradation tag, shows the baseline expression of tsPurple as a basis for comparison. BBa_K2353001 has degradation tag DAS (BBa_M0052) which should cause moderately fast degradation of tsPurple, while BBa_K2353002 has the degradation tag LAA (BBa_M0050), which should cause high rates of degradation due to the high affinity of ClpXP for LAA. | ||
+ | |||
<br><br> | <br><br> | ||
</p> | </p> | ||
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<img style="width:600px; align:center" src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg"> | <img style="width:600px; align:center" src="https://static.igem.org/mediawiki/2017/5/58/Tspurples_gel.jpeg"> | ||
+ | <br> | ||
+ | <i style="font-size: 14px;">Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001</i> | ||
</div> | </div> | ||
Latest revision as of 03:40, 2 November 2017
Composite Parts
The middle part of the full three part construct is one of three tsPurple sequences. Each sequence is composed of BBa_B0034 (RBS) and BBa_K1033906 (tsPurple- a purple chromoprotein also known as TinselPurple). When assembled with p-lambda-r LacI and pLac-ClpXP-CI, the tsPurple constructs are expressed by p-lambda-r LacI. The terminator for tsPurple is located at the beginning of the pLac-ClpXP-CI sequence, before the promoter for ClpXP-CI. When the construct is fully assembled and induced with IPTG, ClpXP is expressed and causes degradation when there is a degradation tag attached at the end of the tsPurple construct. BBa_K2353000, the tsPurple construct without any degradation tag, shows the baseline expression of tsPurple as a basis for comparison. BBa_K2353001 has degradation tag DAS (BBa_M0052) which should cause moderately fast degradation of tsPurple, while BBa_K2353002 has the degradation tag LAA (BBa_M0050), which should cause high rates of degradation due to the high affinity of ClpXP for LAA.
Gel results from a digest of all three tsPurple parts. From top to bottom: BBa_K2353000, BBa_K2353002, BBa_K2353001