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Revision as of 17:12, 31 October 2017


Description


Purpose

When researching synthetic biology, underfunded labs often lack the monetary means to purchase expensive laboratory equipment; basic machinery, such as centrifuges, or more complex materials for analyzing data, such as plate readers, are commodities for many laboratories. The 2017 Lambert iGEM team decided to focus upon these issues, and, therefore, strives to make expensive equipment, like plate readers and centrifuges, more viable resources for underfunded labs.


Method

    The approach Lambert iGEM used was multifaceted:
  • Design a chamber to optimize and standardize conditions for detecting color
  • Write a software application that can be used by anyone to quantify the color values using HSV
  • Test and improve on a low-cost centrifuge
  • Assemble and test these devices with chromoproteins under varying levels of IPTG induction

Using engineering design principles, the 2017 Lambert iGEM team aimed to develop an imaging device called the Chrome-Q to cost-effectively standardize conditions for quantifying chromoprotein data; additionally, the team improved upon the Paperfuge originally designed by the Prakash Lab to pellet cells at a low cost. Different chromoproteins - Tinsel Purple, Virginia Violet and Scrooge Orange - were induced at varying levels of IPTG, causing the cells to express different intensities; pictures of the cells were taken using a Chrome-Q and were measured with a self-constructed software app that analyzes the pigments using HSV color space. Additionally, the team observed that a common issue encountered by synthetic biologists is the overlap of protein expression in development of genetic circuits which negatively affects the quality of performance in a given cell. The 2017 Lambert iGEM Team worked to develop an inducible “switch” to further characterize ClpXP, a non-lysosomal proteolysis mechanism, with three Keio strains; the data showed a correlation between IPTG concentration and amount of protein degradation. Overall, Lambert iGEM desired to promote scientific research under any financial circumstance and to characterize non-lysosomal inducible protein degradation (CLiP’d).
















References

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