Team:Lambert GA/HP/Gold Integrated

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The cost of capital equipment for Synthetic Biology Labs can be prohibitive in certain sectors of research. Labs in developing nations, new universities and high schools struggle with the very real costs of purchasing equipment including: gel electrophoresis equipment, micropipettes, thermocycler, autoclave, microcentrifuge, refrigerated centrifuge, refrigerator, freezer, ultralow freezer, incubators, spectrophotometers, plate readers, among others. These costs don't include the consummable supplies. This is a limiting factor in the development of new synbio ideas.

Lambert was inspired to develop some alternatives for expensive capital equipment: a minimal cost centrifuge inspired by the Prakash Lab paperfuge, and our self designed Chrome-Q and software app. We hope that these alternatives will aid underfunded labs, such as ourselves, and allow research to continue despite funding constraints.

Last year we were granted several Foldscopes from the Prakash Lab at Stanford. These were an exciting development and were a spring board for our own design team. We contacted the lab this past Spring about their low cost centrifuges called the Paperfuge. Dr. Saad Bhamla, then a Postdoctoral student and now an Assistant Professor at Georgia Institute of Technology, shared both paperfuge and 3-D printed fuge designs. Over the course of the year we were able to make modifications to one particular 3-D design that accommodates PCR tubes. The changes we made increased the ease of inserting tubes and we believe will decrease the air resistance. We are waiting for access to a high speed camera to verify the speed increases. The 3-D printed fuge is able to successfully pellet cells in 3 minutes of rotation time using our new model.

Last year our team prototyped a chamber to standardize light conditions in an attempt to quantify RGB values from chromoproteins. This year we were determined to get a working model accompanied by software to analyze the data. Throughout the course of the year five different prototypes were developed, evaluated and improved through a collaboration with Michael Gibson of Gibson Ridge Software. With each prototype, we tested the device and applied engineering principles to improve the design. We developed two Chrome-Q domes optimized for both Android and Apple mobile devices. The Chrome-Q and companion software are a low cost alternative to a plate reader. Instead of using fluorescent reporters our system uses chromoproteins reporters. The Chrome-Q chamber standardizes light conditions with an aperture adjusted for both iPhone and Android cameras , the base allows for comparisons of up to 25 samples at a time, and the software captures the RGB values of the samples and analyzes them in the HSV color space. The HSL values are then normalized using CFU's on standard overnight growth plates.

Throughout the year Lambert engaged in conversations and activities concerning ethics.

Dr. Styczinski's Ideal Prototype for the Zinc Biosensor

To learn more about micronutrient deficiencies around the world, our team met with Dr. Ralph Whitehead, a researcher from the CDC, and he gave our team more insight about the effect of micronutrient deficiencies in public health in lesser developed countries. To learn more about how we integrated our human practices into our project, click here!



Areas of the world most heavily influenced by Micronutrient Deficiencies