Hardware

The Chrome-Q Light Chamber is a 3D-printed imaging measurement system used to quantify results of nutrient deficiency. For our current project, it is also used to quickly and inexpensively measure relative protein degradation through quantification of the color in chromoprotein expression.

One of the most critical variables in imaging today is the consistency of light. Different wavelengths, intensity, power, wattage, and even angling of lights can affect the result of camera imaging. To this end, the Chrome-Q is designed to control these variables of light. The dimensions and dome shape have been optimized for ideal imaging distance and lighting amount. A series circuit was used in order to disrtibute full and equal voltage to each LED in the Chrome-Q.

To use the Chrome-Q, first a sample must be placed on the base under the center of the dome. Then, using the app, a phone can take a picture of the sample through the eyehole, and calculate degradation of protein by analyzing the RGB values in the pixels of the samples.

As a high school lab, one of the many problems we face in research is the lack of funding and monetary support. Sophisticated equipment is incredibly expensive, and so it is very hard to afford the necessary tools and measurement systems for our research. The Chrome-Q Light Chamber is designed to conquer the issue of the cost of a fluorimeter – instead of visualizing with fluorescence, the project uses chromoproteins, which can then be imaged with the chamber we built, and results can be obtained that way. This technology can be used by other teams and facilities for their research, and the full plan of the design is available below so that any team can build it themselves.

Tools

1. 3D Printer (Build Area of at least 7 x 7 x 8in)
2. Non-Reflective Spray Paint(Optional)
3. Camera

1. All the STL files included within the file.
2. LED Lights(3)
3. 9-Volt batteries(2)
4. Wire

For best results, print at a slow speed to ensure the best outcome possible and make sure the 3D printer is tuned correctly. Print each of the Parts at any desired infill percentage and layer height. Also, it’s recommended to print with brims so it does not curl when printing. If need be, the SLDPRT versions are included in the file.

1. Transfer all the STL files into preferred slicing software.
2. Change settings accordingly to the printer.
3. Orient the parts where it can be printed easiest.
4. Convert it into GCODE files.
5. Transfer the GCODE files into preferred printing software.
6. Start Printing.

7. Carefully remove the prints.
8. Sand any defects
9. Test that the dome fits on base.
10. Build series circuit with batteries and LEDs.
11. Align circuit such that LEDs fit into slots.
*Note: If you are interested in 3D-printing the Chrome-Q Light Chamber, please contact us at igem.lamb@gmail.com for the program print files.

Dhakar, L. (n.d.). Image Color Picker (Z. A., Ed.). Retrieved October 10, 2017, from http://www.colorcodepicker.com/

Purple color codes. (n.d.). Retrieved October 10, 2017, from http://www.rapidtables.com/web/color/purple-color.htm

RGB Color Gradient Maker. (n.d.). Retrieved October 10, 2017, from http://www.perbang.dk/rgbgradient/

Tamura, K., Shimada, T., Ono, E., Tanaka, Y., Nagatani, A., Higashi, S., . . . Hara-Nishimura, I. (2003, September). Why green fluorescent fusion proteins have not been observed in the vacuoles of higher plants. The Plant Journal, 35(4), 545-555. doi:10.1046/j.1365-313X.2003.01822.x