Difference between revisions of "Team:Lambert GA/Notebook"
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | ||
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | ||
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Revision as of 11:13, 1 November 2017
Notebook
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Week 1 (Aug 3):
Since we had been having difficulties working with p-lambda-r LacI, we discussed the merits of using a different promoter for our tsPurple constructs.
Week 2 (Aug 7):
After the previous week's discussions, we transformed, inoculated, and miniprepped weak pLac (hoping that a weaker promoter would be less of a strain on our cells). In addition, we attempted to ligate the tsPurples into 1C3, however the miniprep concentrations had been too low, so we needed to order more of our parts from IDT.
Week 3 (Aug 14):
meep
Week 4 (Aug 21):
morp
Week 5 (Aug 28):
meep
Week 6 (Sep 4): morp
Week 7 (Sep 11): meep
Week 8 (Sep 18): morp
Week 9 (Sep 25): maybe delete pics?
Week 10 (Oct 2): meep
Week 11 (Oct 9): morp images? delete?
Week 11 Miniprep R0011--ClpXP--CI and Week 11 Miniprep P-Lambda-R-LacI--GFP--ClpXP-CI
Week 13 (Oct 23): meep
Week 14 (Oct 30): meep
