Difference between revisions of "Team:Lambert GA/Notebook"
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Revision as of 11:27, 1 November 2017
Notebook
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Week 1 (Aug 3):
Since we had been having difficulties working with p-lambda-r LacI, we discussed the merits of using a different promoter for our tsPurple constructs.
Week 2 (Aug 7):
After the previous week's discussions, we transformed, inoculated, and miniprepped weak pLac (hoping that a weaker promoter would cause less strain on our cells). In addition, we attempted to ligate the tsPurples into 1C3, however the miniprep concentrations had been too low, so we needed to order more of our parts from IDT.
Week 3 (Aug 14):
We came to the decision that pLac would not work when our construct was fully assembled because it is the same promoter that is on the second part of our construct. Due to this, we moved back to p-lambda-r LacI and inoculated liquid cultures from a pre-existing plate. In addition, we transformed and then miniprepped our tsPurple constructs, however the concentrations of both p-lambda-r LacI and the tsPurples were shockingly low so we decided to run a PCR next week.
Week 4 (Aug 21):
morp
Week 5 (Aug 28):
meep
Week 6 (Sep 4): morp
Week 7 (Sep 11): meep
Week 8 (Sep 18): morp
Week 9 (Sep 25): maybe delete pics?
Week 10 (Oct 2): meep
Week 11 (Oct 9): morp images? delete?
Week 11 Miniprep R0011--ClpXP--CI and Week 11 Miniprep P-Lambda-R-LacI--GFP--ClpXP-CI
Week 13 (Oct 23): meep
Week 14 (Oct 30): meep
