Difference between revisions of "Team:Lambert GA/Part Collection"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Part Collection </h1>
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<a href="https://2017.igem.org/Team:Lambert_GA" class="dropbtn">Home</a><!--
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      <a href="https://2017.igem.org/Team:Lambert_GA/Team" class="dropbtn">Team</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Team">Team</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Collaborations">Collaborations</a>
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  <a href="https://2017.igem.org/Team:Lambert_GA/Description" class="dropbtn">Project</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Description">Description</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Design">Design</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Experiments">Experiments</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Attributions">Attributions</a>
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      <a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a>
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href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Composite Part">Composite Parts</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a>
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      <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a>
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<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a>
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<a  href="https://igem.org/2017_Judging_Form?team=Lambert_GA"class="dropbtn">Judging Form</a>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
 
  
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<p>
 
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
 
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</p>
 
 
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<h3>Best Part Collection Special Prize</h3>
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<center> <h1 id="MainTitle"><b> Part Collection </b></h1>
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<p>Did your team make a lot of great parts? Is there a team that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection or a collection of awesome pigment parts? Tell the judges you should be evaluated for the Best Parts Collection award! To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts.
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<img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;"></center>
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<br><br>
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If you have a collection of parts you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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<a class="partlink" style="transition: color 0.5s ease-in-out;" href="http://parts.igem.org/Part:BBa_K2353000"> BBa_K2353000: tsPurple </a>
  
  
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<br><br>
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<h4>Note</h4>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<a class="partlink" style="transition: color 0.5s ease-in-out;" href="http://parts.igem.org/Part:BBa_K2353001"> BBa_K2353001: tsPurpleDAS </a>
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<br><br>
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<a class="partlink" style="transition: color 0.5s ease-in-out;" href="http://parts.igem.org/Part:BBa_K2353002"> BBa_K2353002: tsPurpleLAA </a>
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<p style="font-size:20px; text-indent:50px; color: white;">
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Our 2017 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). They were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. The team sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. Lambert iGEM attempted to characterize these previous parts but ran into some obstacles while working with them.</p>
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<br><br>
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<p style="font-size:20px; text-indent:50px; color: white;">
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Lambert iGEM first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. They ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. The team had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS. </p>
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<br><br>
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<p style="font-size:20px; text-indent:50px; color: white;">
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The team sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and they also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it. </p>
 
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<a href="http://www.forsyth.k12.ga.us/lhs"><img src="https://static.igem.org/mediawiki/2017/3/36/RevisedLambertLonghorn.png" style="height:100px;padding:20px;" class="transparency"></a>
  
 
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Latest revision as of 03:36, 2 November 2017


Part Collection


BBa_K2353000: tsPurple

BBa_K2353001: tsPurpleDAS

BBa_K2353002: tsPurpleLAA



Our 2017 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). They were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. The team sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. Lambert iGEM attempted to characterize these previous parts but ran into some obstacles while working with them.



Lambert iGEM first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. They ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. The team had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.



The team sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and they also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.