Difference between revisions of "Team:Lambert GA/Part Collection"
KevinYLi123 (Talk | contribs) (Undo revision 318217 by KevinYLi123 (talk)) |
KevinYLi123 (Talk | contribs) |
||
| (16 intermediate revisions by 5 users not shown) | |||
| Line 33: | Line 33: | ||
div { | div { | ||
| − | color: | + | color: white; |
} | } | ||
| Line 57: | Line 57: | ||
p { | p { | ||
margin: auto; | margin: auto; | ||
| − | + | font-size: 20px; | |
| − | color: | + | color: white; |
} | } | ||
| Line 91: | Line 91: | ||
#MainTitle { | #MainTitle { | ||
padding-top: 50px; | padding-top: 50px; | ||
| − | padding-bottom: | + | padding-bottom: 20px; |
font-size: 45px; | font-size: 45px; | ||
color: #D49AE6; } | color: #D49AE6; } | ||
| Line 102: | Line 102: | ||
body{ | body{ | ||
| − | font-size: | + | font-size:20px; |
} | } | ||
| Line 112: | Line 112: | ||
h2{ | h2{ | ||
| − | color: # | + | color: #D49AE6; |
text-align:center; | text-align:center; | ||
hspace="20" | hspace="20" | ||
| Line 145: | Line 145: | ||
padding: 0px; | padding: 0px; | ||
display: block; | display: block; | ||
| − | margin: 0px auto; | + | margin: 0px auto; |
} | } | ||
| Line 151: | Line 151: | ||
height:50px; | height:50px; | ||
} | } | ||
| + | |||
#sponsors-bottom{ | #sponsors-bottom{ | ||
| Line 296: | Line 297: | ||
opacity:0.4; | opacity:0.4; | ||
} | } | ||
| − | + | .bullet li:before { | |
| − | . | + | content: "•"; |
| − | + | font-size: 170%; | |
| − | + | padding-right: 5px; | |
} | } | ||
| − | |||
</style> | </style> | ||
| Line 334: | Line 334: | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Notebook">Notebook</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/InterLab">InterLab</a> | ||
| − | + | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Results">Results</a><a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Demonstrate">Demonstrate</a> | ||
| Line 343: | Line 343: | ||
</li><!-- | </li><!-- | ||
--><li class="dropdown" > | --><li class="dropdown" > | ||
| − | <a href="https://2017.igem.org/Team:Lambert_GA/ | + | <a href="https://2017.igem.org/Team:Lambert_GA/Part Collection" class="dropbtn">Parts</a> |
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | <a class="drplink" style="transition: color 0.5s ease-in-out;" | + | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Part Collection">Part Collection</a> |
| − | href="https://2017.igem.org/Team:Lambert_GA/ | + | |
<a class="drplink" style="transition: color 0.5s ease-in-out;" | <a class="drplink" style="transition: color 0.5s ease-in-out;" | ||
href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a> | href="https://2017.igem.org/Team:Lambert_GA/Updated_Part">Updated Parts</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Composite Part">Composite Parts</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Composite Part">Composite Parts</a> | ||
| − | + | </div> | |
</li><!-- | </li><!-- | ||
--><!-- | --><!-- | ||
--><a href="https://2017.igem.org/Team:Lambert_GA/Safety"class="dropbtn">Safety</a><!-- | --><a href="https://2017.igem.org/Team:Lambert_GA/Safety"class="dropbtn">Safety</a><!-- | ||
--><li class="dropdown" > | --><li class="dropdown" > | ||
| − | <a href="https://2017.igem.org/Team:Lambert_GA/ | + | <a href="https://2017.igem.org/Team:Lambert_GA/HP/Silver" class="dropbtn">Human Practices</a> |
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | |||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Silver">Silver HP</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/HP/Gold_Integrated">Integrated and Gold</a> | ||
| − | |||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Engagement">Public Engagement</a> | ||
</div> | </div> | ||
| Line 369: | Line 366: | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Applied_Design">Applied Design</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Hardware">Hardware</a> | ||
| − | |||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Model">Model</a> | ||
<a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a> | <a class="drplink" style="transition: color 0.5s ease-in-out;" href="https://2017.igem.org/Team:Lambert_GA/Software">Software</a> | ||
</div> | </div> | ||
</li> | </li> | ||
| − | <a href="https://igem.org/2017_Judging_Form?team=Lambert_GA"class="dropbtn"> | + | <a href="https://igem.org/2017_Judging_Form?team=Lambert_GA"class="dropbtn">Judging Form</a> |
</div> | </div> | ||
| Line 382: | Line 378: | ||
<section> | <section> | ||
| − | |||
| − | |||
| − | |||
| + | <div class="column full_size" style="width:75%; font-size: 20px; margin:auto;" > | ||
| − | + | ||
| + | |||
| + | <br> | ||
<center> <h1 id="MainTitle"><b> Part Collection </b></h1> | <center> <h1 id="MainTitle"><b> Part Collection </b></h1> | ||
<img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;"></center> | <img src="https://static.igem.org/mediawiki/2017/b/bc/T--Lambert_GA--purpleline.png" style="width:18%; margin:auto;"></center> | ||
| Line 419: | Line 415: | ||
<div class="column full_size" style="width:70%; margin:auto;" > | <div class="column full_size" style="width:70%; margin:auto;" > | ||
<div style="color:white"> | <div style="color:white"> | ||
| − | Our 2017 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). | + | <p style="font-size:20px; text-indent:50px; color: white;"> |
| + | Our 2017 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). They were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. The team sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. Lambert iGEM attempted to characterize these previous parts but ran into some obstacles while working with them.</p> | ||
<br><br> | <br><br> | ||
| − | + | <p style="font-size:20px; text-indent:50px; color: white;"> | |
| + | Lambert iGEM first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. They ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. The team had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS. </p> | ||
<br><br> | <br><br> | ||
| − | + | <p style="font-size:20px; text-indent:50px; color: white;"> | |
| + | The team sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and they also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it. </p> | ||
</div> | </div> | ||
<br><br> | <br><br> | ||
Latest revision as of 03:36, 2 November 2017
Our 2017 iGEM team used existing Biobrick parts from the Uppsala team (BBa_K1033906) and also the Endy Lab (BBa_M0050-LAA, M0052-DAS). They were trying to further Edinburgh’s work by expanding upon their experiments with GFP and the degradation tags. The team sought to do this by replacing GFP with the tsPurple chromoprotein and characterizing the ClpXP system. Lambert iGEM attempted to characterize these previous parts but ran into some obstacles while working with them.
Lambert iGEM first obtained the tsPurple sequence from the 2013 Uppsala iGEM wiki page. They ordered the sequence online from IDT, hydrated it, and proceeded to carry out the 3A assembly procedures to create our constructs. The team had the tsPurple with no degradation tag, tsPurple with the LAA degradation tag, and tsPurple with the DAS degradation tag. The degradation tags were obtained from the Endy Lab sequencing and correspond to strong degradation for LAA and moderate for DAS.
The team sought to improve upon the LAA and DAS degradation tags by showing their relative strength in degrading the tsPurple chromoprotein and they also sought to improve upon the characterization of the tsPurple gene so that its relative degradation may be present for anyone who needs it.
