Team:Lambert GA/Updated Part
Updated Parts
The ClpP gene codes for the ClpP subunit protein which works together with the ClpX subunit to break down proteins that are tagged with degradation tags. The ClpXP system identifies tagged proteins and proceeds to unfold the protein and break it down into individual amino acids. The ClpXP system along with degradation tags can be used to break down certain proteins which are produced by certain genes of interest and it has a range of applications such as measuring how fast a certain protein can be degraded in a cell. The ClpP sequence which was pulled from the E. coli genome contained illegal restriction enzyme sites. In order to remove these illegal sites, wobbles were introduced into the ClpP sequence so that the gene is not cut anywhere and is allowed to function properly. ClpP is a naturally present gene in the E. coli genome.
