Difference between revisions of "Team:Lethbridge HS/Results"
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<p> <i> Figure 1. 1% agarose gel of melA_pJET digested with EcoRI and PstI. Plasmids 1 and 3 had the melA construct successfully insert into pJET without disruption of the cut sites.</i></p> | <p> <i> Figure 1. 1% agarose gel of melA_pJET digested with EcoRI and PstI. Plasmids 1 and 3 had the melA construct successfully insert into pJET without disruption of the cut sites.</i></p> | ||
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| + | <p> We then proceeded through cloning methods to finally insert the melA expression construct into pSB1C3 for submission to the iGEM registry. Were able to generate two successful clones (Figure 2, melA_pSC1C3 5 and 6, boxed). One of these clones was sequence confirmed and submitted to the Parts Registry (BBa_INSERT LINK). | ||
<center><img src="https://static.igem.org/mediawiki/2017/b/bc/--Lethbridge_HS--melA_and_indB_pSB1C3.png" width="500px" height="800px"class="img-responsive"> | <center><img src="https://static.igem.org/mediawiki/2017/b/bc/--Lethbridge_HS--melA_and_indB_pSB1C3.png" width="500px" height="800px"class="img-responsive"> | ||
</center> | </center> | ||
Revision as of 02:20, 1 November 2017
Part Development
We decided to prioritize the assembly of one construct, the melanin construct, because black is the most commonly used ink colour. We designed a g-block to contain assembled promoter, RBS, melA and terminator parts, including the BioBrick prefix and suffix sequences. We first cloned that into the pJET plasmid using the ligation kit from Thermo-Fisher Scientific. We chose this method because this kit is very efficient and it would improve our chances of successful assembly. We did succeed in inserting the melA construct into pJET (Figure 1).
Figure 1. 1% agarose gel of melA_pJET digested with EcoRI and PstI. Plasmids 1 and 3 had the melA construct successfully insert into pJET without disruption of the cut sites.
We then proceeded through cloning methods to finally insert the melA expression construct into pSB1C3 for submission to the iGEM registry. Were able to generate two successful clones (Figure 2, melA_pSC1C3 5 and 6, boxed). One of these clones was sequence confirmed and submitted to the Parts Registry (BBa_INSERT LINK).
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