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| <article class="card"> | | <article class="card"> |
| <p class="date">Week 26 - 2017/06/28 - 2017/06/30</p> | | <p class="date">Week 26 - 2017/06/28 - 2017/06/30</p> |
− | <h5>Obtaining the pETDuet-1 plasmid backbone</h5>
| |
| <ul> | | <ul> |
| + | <li>Obtaining the pETDuet-1 plasmid backbone</li> |
| <li>Cell cultivation (<em>E. coli</em> BL21-DE3) with pETDuet-1 plasmid </li> | | <li>Cell cultivation (<em>E. coli</em> BL21-DE3) with pETDuet-1 plasmid </li> |
| <li>Plasmid isolation (pETDuet-1) using GeneJET Plasmid Miniprep Kit </li> | | <li>Plasmid isolation (pETDuet-1) using GeneJET Plasmid Miniprep Kit </li> |
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| <li>Inoculation of cells containing GFP1-9 device, sfGFP and B21 competent cells to 5 mL LB media</li> | | <li>Inoculation of cells containing GFP1-9 device, sfGFP and B21 competent cells to 5 mL LB media</li> |
| <li>Freezing and thawing of the overnight culture</li> | | <li>Freezing and thawing of the overnight culture</li> |
| + | <li>SDS-PAGE to check for the expression of the proteins</li> |
| </ul> | | </ul> |
| </article> | | </article> |
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| <p class="date"> Week 43 (2017/10/23 - 2017/10/29)</p> | | <p class="date"> Week 43 (2017/10/23 - 2017/10/29)</p> |
| <ul> | | <ul> |
− | <li>SDS-PAGE to check for the expression of the proteins</li>
| |
| <li>Transformation of the samples being ligated overnight using quick and standard protocol</li> | | <li>Transformation of the samples being ligated overnight using quick and standard protocol</li> |
| <li>Colony PCR to check wether ligation succeeded</li> | | <li>Colony PCR to check wether ligation succeeded</li> |