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The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
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          <p class="header">Pattrick Wilke</p>
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            <span class="date">Masters Biochemistry (TUM)</span>
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            He is currently in the Master in Biochemistry at TUM and is part of our Wet Lab Team. After spending too much time in the lab he has developed some side effects: he turned into a pipetting-robot and started to perceive reality in terms of incubation times. While he waits for the experiments to be done, he likes to play table football with Julian (you will know about him soon). Also, his laugh is very contagious.
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Revision as of 10:58, 13 September 2017


Abstract

Pattrick Wilke

Masters Biochemistry (TUM)
three dots

He is currently in the Master in Biochemistry at TUM and is part of our Wet Lab Team. After spending too much time in the lab he has developed some side effects: he turned into a pipetting-robot and started to perceive reality in terms of incubation times. While he waits for the experiments to be done, he likes to play table football with Julian (you will know about him soon). Also, his laugh is very contagious.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.

Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

| The iGem Munich 2017 Team | 2017.07.13 |