Difference between revisions of "Team:Munich/Testflow"

Line 1: Line 1:
<!-- #919191 Grau1 -->
 
<!-- #787878 Grau2 -->
 
<!-- #51A7f9 Blau1 -->
 
<!-- #3c7cb9 Blau2 -->
 
<!-- #ffffff weiss -->
 
 
<html>
 
<html>
<link rel="stylesheet" type="text/css"
 
href="https://2017.igem.org/Template:Munich/CSS?action=raw&ctype=text/css" />
 
<link rel="stylesheet" type="text/css"
 
href="https://2017.igem.org/Template:Munich/Header?action=raw&ctype=text/css" />
 
<link rel="stylesheet" type="text/css"
 
href="https://2017.igem.org/Template:Munich/Filter?action=raw&ctype=text/css" />
 
 
<head>
 
<head>
<style>
+
<style type="text/css">
#HQ_page h3{
+
html, body
text-align: left;
+
{
margin-bottom: 10px;
+
margin: 0;
 +
padding: 0;
 +
max-width: 100%;
 +
overflow-x: hidden;
 +
font-family: Helvetica, Arial, sans-serif;
 
}
 
}
 
#HQ_page h3 a{
 
color: #51a7f9;
 
}
 
 
#HQ_page h3 a:hover{
 
color: #3c7cb9;
 
}
 
 
#myContent *{
 
color: #919191;
 
}
 
 
#myContent td p{
 
margin-bottom: 20px;
 
}
 
 
 
</style>
 
</style>
 
</head><body>
 
</head><body>
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
 
  
<!-- Bar -->
+
 
<tr><td height="50" bgcolor=#ffffff></td><td class="no-padding" bgcolor=#ffffff height="32" width="960" align=center>
+
<div class="dropdown">
+
  <a href="/Team:Munich"><button class="dropbtnCascAID dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"></button></a>
+
</div><div class="dropdown">
+
  <a href="project"><button class="dropbtnProject dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Project</b></p></button></a>  <div class="dropdown-contentDescription dropdown-content">
+
    <a href="description"><p><b>Description</b></p></a>
+
  </div>
+
  <div class="dropdown-contentDesign dropdown-content">
+
    <a href="design"><p><b>Design</b></p></a>
+
  </div>
+
  <div class="dropdown-contentProofofConcept dropdown-content">
+
    <a href="proofofconcept"><p><b>Proof of Concept</b></p></a>
+
  </div>
+
  <div class="dropdown-contentDemonstration dropdown-content">
+
    <a href="demonstration"><p><b>Demonstration</b></p></a>
+
  </div>
+
  <div class="dropdown-contentApplication dropdown-content">
+
    <a href="application"><p><b>Application</b></p></a>
+
  </div>
+
  <div class="dropdown-contentFinalResults dropdown-content">
+
    <a href="finalresults"><p><b>Final Results</b></p></a>
+
  </div>
+
  <div class="dropdown-contentEntrepreneurship dropdown-content">
+
    <a href="entrepreneurship"><p><b>Entrepeneurship</b></p></a>
+
  </div>
+
  <div class="dropdown-contentAchievements dropdown-content">
+
    <a href="achievements"><p><b>Achievements</b></p></a>
+
  </div>
+
</div><div class="dropdown" valign=center>
+
<a href="wetlab"><button class="dropbtnWetlab dropbtn" valign=center><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Wetlab</b></p></button></a>
+
  <div class="dropdown-contentProtocolsMethods dropdown-content">
+
    <a href="protocolsmethods"><p><b>Protocols and<br> Methods</b></p></a>
+
  </div>
+
  <div class="dropdown-contentMaterials dropdown-content">
+
    <a href="materials"><p><b>Materials</b></p></a>
+
  </div>
+
  <div class="dropdown-contentLabJournal dropdown-content">
+
    <a href="labjournal"><p><b>LabJournal</b></p></a>
+
  </div>
+
  <div class="dropdown-contentSafety dropdown-content">
+
    <a href="safety"><p><b>Safety</b></p></a>
+
  </div>
+
  <div class="dropdown-contentParts dropdown-content">
+
    <a href="parts"><p><b>Parts</b></p></a>
+
  </div>
+
  <div class="dropdown-contentInterlab dropdown-content">
+
    <a href="interlab"><p><b>Interlab</b></p></a>
+
  </div>
+
</div><div class="dropdown">
+
  <a href="model"><button class="dropbtnModel dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Modelling</b></p></button></a></div><div class="dropdown">
+
  <a href="softhardware"><button class="dropbtnSoftHardware dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Software/Hardware</b></p></button></a>
+
  <div class="dropdown-contentSoftware dropdown-content">
+
    <a href="software"><p><b>Software</b></p></a>
+
  </div>
+
  <div class="dropdown-contentHardware dropdown-content">
+
    <a href="hardware"><p><b>Hardware</b></p></a>
+
  </div>
+
</div><div class="dropdown">
+
  <a href="hp"><button class="dropbtnHP dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Human Practices</b></p></button></a>
+
  <div class="dropdown-contentHPSilver dropdown-content">
+
    <a href="hpsilver"><p><b>Human Practices (Silver)</b></p></a>
+
  </div>
+
  <div class="dropdown-contentIHPgold dropdown-content">
+
    <a href="ihpgold"><p><b>Human Practices (Gold)</b></p></a>
+
  </div>
+
  <div class="dropdown-contentPublicEngagement dropdown-content">
+
    <a href="publicengagement"><p><b>Public Engagement</b></p></a>
+
  </div>
+
  <div class="dropdown-contentCollaborations dropdown-content">
+
    <a href="collaborations"><p><b>Collaborations</b></p></a>
+
  </div>
+
</div><div class="dropdown">
+
  <a href="/Team:Munich/Team"><button class="dropbtnTeam dropbtn"><img src="https://static.igem.org/mediawiki/2017/0/08/T--Munich--Overlay.png"><p><b>Team</b></p></button></a>
+
  <div class="dropdown-contentTeamMembers dropdown-content">
+
    <a href="/Team:Munich/Team"><p><b>Team members</b></p></a>
+
  </div>
+
  <div class="dropdown-contentSupervisors dropdown-content">
+
    <a href="supervisors"><p><b>Supervisors</b></p></a>
+
  </div>
+
  <div class="dropdown-contentPIs dropdown-content">
+
    <a href="supervisors"><p><b>PIs</b></p></a>
+
  </div>
+
  <div class="dropdown-contentCollaboratingInstitutions dropdown-content">
+
    <a href="supervisors"><p><b>Institutions</b></p></a>
+
  </div>
+
  <div class="dropdown-contentSponsors dropdown-content">
+
    <a href="sponsors"><p><b>Sponsor
+
s</b></p></a>
+
  </div>
+
  <div class="dropdown-contentAttributions dropdown-content">
+
    <a href="/Team:Munich/Team"><p><b>Attributions</b></p></a>
+
  </div>
+
</div>
+
</td><td height="32" bgcolor=#ffffff></td></tr>
+
<!-- Content -->
+
<tr><td width="100%" colspan=3>
+
 
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
 
<table width=100% height=100% cellpadding=0 cellspacing=0 border=0>
<tr>
+
<tr>
<td width="40%">
+
<td width="40%">
</td>
+
</td>
<td id="myContent" width="20%" valign=top align=center>
+
<td width="20%" valign=bottom>
<br>
+
<!-- Head End -->
+
<!-- Content Begin -->
+
<img id="TopPicture" width="960" src="https://static.igem.org/mediawiki/2017/a/a5/T--Munich--FrontPagePictures_Sponsors.jpg" style="margin-bottom: 15px">
+
<table width="900" border=0 cellspacing=0 cellpadding=10>
+
<tr><td colspan=3 align=left valign=center>
+
<font size=7 color=#51a7f9><b style="color: #51a7f9">Sponsors</b></font>
+
</td></tr>
+
<tr>
+
<td  colspan = 3 align="left">
+
<p class="introduction">
+
We would like to thank all our sponsors for their generous financial support and helpful guidance.
+
Thanks to them we were able to develop CascAID. Their
+
sponsorship allowed us to make the most out of the iGEM competition and attend the Giant Jamboree. We are honored to name this institutions and companies our sponsors. </p>
+
  
</td>
 
</tr>
 
<tr><td><h2>Academic Sponsors and Institutions</h2></td></tr>
 
  
<tr><td align=center valign=center>
 
<h3><a href="https://www.tum.de/">Technische Universität München (TUM)</a></h3>
 
<p> 
 
One of Europe's leading universities, the TUM is an institution known worldwide for its excellence in
 
education and research. With a strong focus on promoting new ideas and innovation, the TUM offers
 
their students an impressive network of industry and academic partners around the world both during
 
their studies and after graduation. Their accomplishments in teaching and research routinely award
 
them top spots in national and international rankings.
 
</p>
 
  
  
</td>
+
<!-- Content Begin -->
<td align=center valign=center>
+
<a href="https://www.tum.de/"><img src="https://static.igem.org/mediawiki/2017/1/1e/T--Munich--wiki_image_coop_tum_high.svg" width="270" height="128"></a>
+
</td></tr>  
+
  
  
<tr><td align=center valign=center>
+
<video height="270" width="960" autoplay="autoplay" controls>
<h3><a href="https://www.uni-muenchen.de/index.html">Ludwig-Maximilians-Universität München (LMU)</a></h3>
+
<source src="video.mp4" type="video/mp4">
<p> 
+
Your browser does not support the video tag.
Founded in 1472, the LMU has since its beginnings been committed to offer their students outstanding
+
</video>
education and promoting the discovery of new and innovative theories. By supporting their students
+
and researchers in their scientific endeavors, the LMU upholds this tradition to date, which shows in
+
their reputation as one of the top universities in Europe and their recognition as a University of
+
Excellence in Germany.
+
</p>
+
  
  
</td>
 
<td align=center valign=center>
 
<a href="https://www.uni-muenchen.de/index.html"><img src="https://static.igem.org/mediawiki/2017/0/0d/T--Munich--Logo_LMU.png" width="270"></a>
 
</td></tr>
 
  
<tr><td align=center valign=center>
 
<h3><a href="http://www.uni-muenchen.de/studium/lehre_at_lmu/index.html">Lehre @ LMU</a></h3>
 
<p> 
 
Lehre @ LMU is a university-wide programme that fosters the education of LMU students by promoting scientific research and innovative ways of teaching. Through Lehre @ LMU students can learn more about the academic world thanks to research-focused courses, special faculty side-projects and diverse mentoring and tutoring programmes. Students' own ideas and projects are also supported financially and logistically by an interdisciplinary network of students and experts.
 
</p>
 
  
 +
<table width="960" border=0 cellspacing=10 cellpadding=10>
  
</td>
+
<tr><td colspan=2 align=right valign=center height=10>
<td align=center valign=center>
+
<font size=2>Date</font>
<a href="http://www.uni-muenchen.de/studium/lehre_at_lmu/index.html"><img src="https://static.igem.org/mediawiki/2017/9/9a/T--Munich--Logo_LehreLMU.gif" width="200"></a>
+
</td></tr>
</td></tr>  
+
<tr><td colspan=2 align=left valign=center>
 
+
<font size=7><p style="text-align:justify">Abstract</p></font>
 +
</td></tr>
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="https://www.nano-initiative-munich.de/">Nanosystems Initiative München (NIM)</a></h3>
+
<p style="text-align:justify">
<p> 
+
The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.  
The NIM cluster is a member of the Excellence Initiative of the German government. Its goal is to bring together the expertise and experience of scientists in the fields of physics, biochemistry, electrical engineering and medicine among others to develop nanoscale building blocks and integrate them into coherent nanosystems with a wide and powerful range of applications. In addition, NIM promotes scientific careers by supporting student projects and offering graduate programs for young scientists.
+
</td><td align=center valign=center>
</p>
+
<img src="placeholder.jpg" width="420">
 
+
</td></tr>
 
+
</td>
+
<td align=center valign=center>
+
<a href="https://www.nano-initiative-munich.de/"><img src="https://static.igem.org/mediawiki/2017/a/a7/T--Munich--wiki_image_sponsors_nim.png" width="270"></a>
+
</td></tr>  
+
 
+
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="http://www.grk2062.uni-muenchen.de/index.html">GRK2062 Molecular Principles of Synthetic Biology</a></h3>
+
<p style="text-align:justify">
<p> 
+
Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.
The GRK2062 is a Research Training group that provides an innovative PhD training program in Synthetic Biology with a focus in  interdisciplinary education. By promoting collaboration of scientists in the fields of physics, biology and chemistry the GRK2062 has a wide array of research projects that properly mirror the interdisciplinary nature of Synthetic Biology. The communication between the different fields is facilitated thanks to the holistic programme of both practical and theoretical courses that the GRK2062 offers their doctoral researches.
+
 
</p>
 
</p>
 
+
</td><td align=center valign=center>
 
+
<p style="text-align:justify">
</td>
+
Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.
<td align=center valign=center>
+
<a href="http://www.grk2062.uni-muenchen.de/index.html"><img src="https://static.igem.org/mediawiki/2017/0/00/T--Munich--Logo_GRK.jpg" width="270"></a>
+
</td></tr>
+
 
+
<tr><td><h2>Industry sponsors</h2></td></tr>
+
 
+
<tr><td align=center valign=center>
+
<h3><a href="https://www.idtdna.com/">Integrated DNA Technologies (IDT)</a></h3>
+
<p>
+
Integrated DNA Technologies is one of the largest manufacturers of oligonucleotides in the world,
+
offering also solutions in genome editing, gene synthesis and sequencing. IDT is a reliable partner for
+
oligo-synthesis and, thanks to its vast expertise and experience, delivers a high-quality product in record
+
time. Many of our cloning and sequencing primers were synthesized by them and without their help,
+
this project would not have been possible.
+
 
</p>
 
</p>
 +
</td></tr>
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Authors</font><hr>
 +
</td></tr>
  
  
</td>
 
<td align=center valign=center>
 
<a href="https://www.idtdna.com/"><img src="https://static.igem.org/mediawiki/2017/1/1a/T--Munich--wiki_image_sponsors_idt.png" width="270"></a>
 
</td></tr>
 
  
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Date</font>
 +
</td></tr>
 +
<tr><td colspan=2 align=left valign=center>
 +
<font size=7><p style="text-align:justify">Headline</p></font>
 +
</td></tr>
 +
<tr><td align=center valign=center colspan=2>
 +
<img src="placeholder.jpg" width="900">
 +
</td></tr>
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="http://www.biomers.net/">biomers.net</a></h3>
+
<p style="text-align:justify">
<p>
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
biomers.net specializes in the synthesis of oligonucleotides of all kinds. From primers to modified RNAsequences,
+
biomers.net is an amazing partner for all scientist working with nucleotides. Their rigorous
+
quality control and helpful support guarantee that their products are always a good choice. We are
+
deeply grateful for their generous sponsorship, which allowed us to synthesize primers, target RNA and
+
crRNA sequences, among others.
+
 
</p>
 
</p>
 
+
</td><td align=center valign=center>
 
+
<p style="text-align:justify">
</td>
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
<td align=center valign=center>
+
<a href="http://www.biomers.net/"><img src="https://static.igem.org/mediawiki/2017/a/a9/T--Munich--wiki_image_sponsors_biomers.png" width="270"></a>
+
</td></tr>
+
 
+
<tr><td align=center valign=center>
+
<h3><a href="https://www.neb.com/">New England Biolabs</a></h3>
+
<p> 
+
New England Biolabs provides many labs around the world with enzymes and a wide array of essential
+
buffers for life sciences applications. As an industry leader, NEB has become one of the best suppliers for
+
kits and enzymatic assays. We would like to thank them for their products, as their enzymes played a
+
central part on our experiments and their kits and assays were important benchmarks.
+
 
</p>
 
</p>
 +
</td></tr>
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Authors</font><hr>
 +
</td></tr>
  
 +
<hr>
  
</td>
+
<tr><td colspan=2 align=right valign=center height=10>
<td align=center valign=center>
+
<font size=2>Date</font>
<a href="https://www.neb.com/"><img src="https://static.igem.org/mediawiki/2017/8/87/T--Munich--wiki_image_sponsors_neb.svg" width="270"></a>
+
</td></tr>
</td></tr>  
+
<tr><td colspan=2 align=left valign=center>
 
+
<font size=7><p style="text-align:justify">Headline</p></font>
 
+
</td></tr>
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="https://www.gatc-biotech.com/en/index.html">GATC Biotech</a></h3>
+
<p style="text-align:justify">
<p> 
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
GATC Biotech is one of Europe's most renowned sequencing companies. Founded in 1990, they were
+
one of the first providers of non-radioactive sequencing and have been innovating in the field ever since.
+
Be it transcriptomics, genomics, single samples or whole genomes, GATC Biotech presents a high-quality
+
solution. Many thanks to GATC Biotech for offering their sequencing service to us and helping us
+
validate our plasmids and BioBricks.
+
 
</p>
 
</p>
 
+
</td><td align=center valign=center rowspan=2>
 
+
<img src="placeholder.jpg" height="600" width="420">
</td>
+
</td></tr>
<td align=center valign=center>
+
<a href="https://www.gatc-biotech.com/en/index.html"><img src="https://static.igem.org/mediawiki/2017/5/59/T--Munich--wiki_image_sponsors_gatc.png" width="270"></a>
+
</td></tr>  
+
 
+
 
+
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="https://www.promega.com/">Promega</a></h3>
+
<p style="text-align:justify">
<p> 
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
Promega is a worldwide provider of technical solutions for researchers worldwide. With a wide portfolio
+
of over 3000 products covering genomics, cellular expression, and drug discovery, among others,
+
Promega is a strong partner for life sciences researchers. Promega's proprietary technologies also
+
present unique and powerful solutions for research and the development of new ideas. We thank
+
Promega for their RNA purification kits and enzymes.
+
 
</p>
 
</p>
 +
</td></tr>
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Authors</font><hr>
 +
</td></tr>
  
  
</td>
 
<td align=center valign=center>
 
<a href="https://www.promega.com/"><img src="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--wiki_image_sponsors_promega.png" width="270"></a>
 
</td></tr>
 
  
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Date</font>
 +
</td></tr>
 +
<tr><td colspan=2 align=left valign=center>
 +
<font size=7><p style="text-align:justify">Headline</p></font>
 +
</td></tr>
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="http://www.scienova.com/xanario/">Scienova</a></h3>
+
<p style="text-align:justify">
<p> 
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
Scienova is a company quickly rising to become a staple of sample preparation disposables. Specializing
+
in disposable dialysis material, Scienova offers a simple and affordable solution to protein purification.
+
We are greatly thankful to Scienova for providing us with their amazing dialysis membrane that we used
+
for protein purification of Cas13a.
+
 
</p>
 
</p>
 
+
</td><td align=center valign=center>
 
+
<img src="placeholder.jpg" width="420">
</td>
+
</td></tr>
<td align=center valign=center>
+
<a href="http://www.scienova.com/xanario/"><img src="https://static.igem.org/mediawiki/2017/3/3b/T--Munich--wiki_image_sponsors_scienova.png" width="270"></a>
+
</td></tr>  
+
 
+
 
<tr><td align=center valign=center>
 
<tr><td align=center valign=center>
<h3><a href="https://www.eurofins.com/">Eurofins Genomics</a></h3>
+
<p style="text-align:justify">
<p> 
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
Eurofins Genomics is an international provider of genomic services. With their proprietary technologies
+
and protocols, they offer high quality next generation sequencing, gene synthesis, DNA oligos, RNA
+
oligos and many other solutions all-around genomics. A comprehensive client support and an extensive
+
portfolio makes Eurofins Genomics a strong partner for both research and industrial fields. Their
+
overnight sequencing and oligo synthesis services were a great help for our project. We are greatly
+
thankful for their support for our team´s t-shirts.
+
 
</p>
 
</p>
 
+
</td><td align=center valign=center colspan=2>
 
+
<p style="text-align:justify">
</td>
+
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."
<td align=center valign=center>
+
<a href="https://www.eurofins.com/"><img src="https://static.igem.org/mediawiki/2017/6/65/T--Munich--Logo_Eurofins.png" width="270"></a>
+
</td></tr>
+
 
+
 
+
<tr><td align=center valign=center>
+
<h3><a href="http://www.gelifesciences.com">GE Healthcare Life Sciences</a></h3>
+
<p
+
GE Healthcare Life Sciences has the mission of pushing the medical field further by providing products
+
and solutions ranging from nucleic acid research and microscopy to quality control and cell therapy.
+
They also support and help develop new technologies that will become future standards. We would like
+
to thank GE Healthcare Life Sciences for providing us with different membranes and materials that came
+
to be an integral part of CascAID and for their guidance and consulting.</p>
+
 
+
 
+
</td>
+
<td align=center valign=center>
+
<a href="http://www.gelifesciences.com"><img src="https://static.igem.org/mediawiki/2017/1/1b/T--Munich--wiki_image_sponsors_gehealthcare.svg" width="270"></a>
+
</td></tr>
+
 
+
 
+
 
+
<tr><td align=center valign=center>
+
<h3><a href="https://www.carlroth.com/">Carl Roth</a></h3>
+
<p> 
+
Carl Roth has provided laboratories around the word with quality equipment and highly pure chemicals
+
for over 130 years. Measuring and optical instruments, cleaning products, laboratory appliances, but
+
also chemicals for broth and gel preparation among much more, Carl Roth has everything a scientist
+
needs. We thank Carl Roth for supplying us with great equipment and chemicals.
+
 
</p>
 
</p>
 +
</td></tr>
 +
<tr><td colspan=2 align=right valign=center height=10>
 +
<font size=2>Authors</font>
 +
</td></tr>
  
  
</td>
 
<td align=center valign=center>
 
<a href="https://www.carlroth.com/"><img src="https://static.igem.org/mediawiki/2017/d/d6/T--Munich--wiki_image_sponsors_roth.png" width="140"></a>
 
</td></tr>
 
  
 +
</table>
  
  
  
 
<tr><td align=center valign=center>
 
<h3><a href="https://www.qiagen.com/">Qiagen</a></h3>
 
<p> 
 
Qiagen is true to their Sample to Insight framework and offers assay technologies capable of delivering
 
valuable and reliable insights. Qiagen has helped researchers since the early days of biotechnology and
 
contributed to the field greatly by cutting down hands-on time for fundamental procedures like plasmid
 
isolation. As one of the many users of Qiagen's kits, we are thankful for their support and collaboration,
 
especially in the field of plasmid purification.
 
</p>
 
 
 
</td>
 
<td align=center valign=center>
 
<a href="https://www.qiagen.com/"><img src="https://static.igem.org/mediawiki/2017/9/99/T--Munich--wiki_image_sponsors_qiagen.svg" width="140"></a>
 
</td></tr>
 
 
 
<tr><td align=center valign=center>
 
<h3><a href="https://www.unternehmertum.de/index.html?lang=en">UnternehmerTUM</a></h3>
 
<p> 
 
UnternehmerTUM is a company with a clear goal: help entrepreneurs to develop their
 
ideas and make successful start-up out of them. UnternehmerTUM is a valuable partner for everyone
 
with an idea and an ambition, as they provide support and guidance in each step of the way, from the
 
initial plan to going public. Currently, UnternehmerTUM helps to start more than 50 start-ups per year
 
and counts with the collaboration of 10 industry partners. With their great part catalog, other materials and courses from MakerSpace, developing our hardware would have been
 
impossible.
 
</p>
 
 
 
</td>
 
<td align=center valign=center>
 
<a href="https://www.unternehmertum.de/index.html?lang=en"><img src="https://static.igem.org/mediawiki/2017/b/b2/T--Munich--wiki_image_sponsors_unternehmertum.png" width="270"></a>
 
</td></tr>
 
<tr><td class="no-padding" colspan=2 align=right valign=center height=10>
 
<br><br><br><center><hr></center>
 
</td></tr>
 
</table>
 
 
<!-- Content End -->
 
<!-- Content End -->
  
  
  
 +
<!-- Footer Begin -->
  
 +
<hr>
  
 +
<table width=960 height=35 cellpadding=0 cellspacing=0 border=0><tr><td align=right valign=center>
  
 
+
<table width=200 height=35 cellpadding=0 cellspacing=15 border=0><tr>
 
+
 
+
 
+
 
+
 
+
 
+
 
+
<!-- Foot Begin -->
+
<table id="myFooter" width=960 height=35 cellpadding=0 cellspacing=0 border=0><tr><td align=right class="no-padding" valign=center>
+
 
+
<table width=300 height=35 cellpadding=0 cellspacing=0 border=0><tr>
+
  
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
 
<a href="https://www.en.uni-muenchen.de/index.html" target="_blank3" >
<img src="https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/f/fd/T--Munich--wiki_image_coop_lmu_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/83/T--Munich--wiki_image_coop_lmu_low.svg'">
+
<img src="wiki_image_coop_lmu_low.svg" border=0 height="20" onmouseover="this.src='wiki_image_coop_lmu_high.svg'" onmouseout="this.src='wiki_image_coop_lmu_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 445: Line 163:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://www.tum.de/" target="_blank4" >
 
<a href="https://www.tum.de/" target="_blank4" >
<img src="https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/1/1e/T--Munich--wiki_image_coop_tum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/ae/T--Munich--wiki_image_coop_tum_low.svg'">
+
<img src="wiki_image_coop_tum_low.svg" border=0 height="20" onmouseover="this.src='wiki_image_coop_tum_high.svg'" onmouseout="this.src='wiki_image_coop_tum_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 451: Line 169:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
 
<a href="http://www.e14.ph.tum.de/en/home/" target="_blank5" >
<img src="https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/72/T--Munich--wiki_image_coop_e14_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/a/a3/T--Munich--wiki_image_coop_e14_low.svg'">
+
<img src="wiki_image_coop_e14_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_coop_e14_high.svg'" onmouseout="this.src='wiki_image_coop_e14_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 457: Line 175:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="http://www.biochem.mpg.de/en" target="_blank5" >
 
<a href="http://www.biochem.mpg.de/en" target="_blank5" >
<img src="https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='https://static.igem.org/mediawiki/2017/d/df/T--Munich--wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/d/da/T--Munich--wiki_image_coop_maxplanck_low.svg'">
+
<img src="wiki_image_coop_maxplanck_low.svg" border=0 height="30" onmouseover="this.src='wiki_image_coop_maxplanck_high.svg'" onmouseout="this.src='wiki_image_coop_maxplanck_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 463: Line 181:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
 
<a href="https://www.helmholtz-muenchen.de/" target="_blank5" >
<img src="https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg" border=0 height="20" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6b/T--Munich--wiki_image_coop_helmholtzzentrum_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/05/T--Munich--wiki_image_coop_helmholtzzentrum_low.svg'">
+
<img src="wiki_image_coop_helmholtzzentrum_low.svg" border=0 height="20" onmouseover="this.src='wiki_image_coop_helmholtzzentrum_high.svg'" onmouseout="this.src='wiki_image_coop_helmholtzzentrum_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 469: Line 187:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://igem.org/Main_Page" target="_blank6" >
 
<a href="https://igem.org/Main_Page" target="_blank6" >
<img src="https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_coop_igem_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/0/0f/T--Munich--wiki_image_coop_igem_low.svg'">
+
<img src="wiki_image_coop_igem_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_coop_igem_high.svg'" onmouseout="this.src='wiki_image_coop_igem_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
  
</tr></table></td><td class="no-padding" align=center valign=center><table width=35 height=35 cellpadding=0 cellspacing=0 border=0><tr><td width="35" align=center valign=center></td></tr></table></td><td class="no-padding" align=left valign=center><table width=200 height=35 cellpadding=0 cellspacing=0 border=0><tr>
+
</tr></table>
 +
 
 +
</td><td align=center valign=center>
 +
 
 +
<table width=35 height=35 cellpadding=0 cellspacing=0 border=0><tr>
 +
 
 +
<td width="35" align=center valign=center>
 +
</td>
 +
 
 +
</tr></table>
 +
 
 +
</td><td align=left valign=center>
 +
 
 +
<table width=200 height=35 cellpadding=0 cellspacing=15 border=0><tr>
  
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://www.facebook.com/Munich.iGEM" target="_blank7" >
 
<a href="https://www.facebook.com/Munich.iGEM" target="_blank7" >
<img src="https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/4/44/T--Munich--wiki_image_sm_facebook_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/76/T--Munich--wiki_image_sm_facebook_low.svg'">
+
<img src="wiki_image_sm_facebook_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_sm_facebook_high.svg'" onmouseout="this.src='wiki_image_sm_facebook_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 483: Line 214:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://twitter.com/iGEM_Munich" target="_blank8" >
 
<a href="https://twitter.com/iGEM_Munich" target="_blank8" >
<img src="https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/c/cc/T--Munich--wiki_image_sm_twitter_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/8/8b/T--Munich--wiki_image_sm_twitter_low.svg'">
+
<img src="wiki_image_sm_twitter_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_sm_twitter_high.svg'" onmouseout="this.src='wiki_image_sm_twitter_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 489: Line 220:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="mailto:igem.munich@gmail.com">
 
<a href="mailto:igem.munich@gmail.com">
<img src="https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/9/93/T--Munich--wiki_image_sm_mail_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/7/79/T--Munich--wiki_image_sm_mail_low.svg'">
+
<img src="wiki_image_sm_mail_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_sm_mail_high.svg'" onmouseout="this.src='wiki_image_sm_mail_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 495: Line 226:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://www.instagram.com/igem.munich.2017/" target="_blank9" >
 
<a href="https://www.instagram.com/igem.munich.2017/" target="_blank9" >
<img src="https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/6e/T--Munich--wiki_image_sm_instagram_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/e/e9/T--Munich--wiki_image_sm_instagram_low.svg'">
+
<img src="wiki_image_sm_instagram_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_sm_instagram_high.svg'" onmouseout="this.src='wiki_image_sm_instagram_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
Line 501: Line 232:
 
<td align=center valign=center>
 
<td align=center valign=center>
 
<a href="https://2017.igem.org/Team:Munich" target="_blank10" >
 
<a href="https://2017.igem.org/Team:Munich" target="_blank10" >
<img src="https://static.igem.org/mediawiki/2017/1/1d/T--Munich--wiki_image_sm_web_low.svg" border=0 height="25" onmouseover="this.src='https://static.igem.org/mediawiki/2017/6/61/T--Munich--wiki_image_sm_web_high.svg'" onmouseout="this.src='https://static.igem.org/mediawiki/2017/1/1d/T--Munich--wiki_image_sm_web_low.svg'">
+
<img src="wiki_image_sm_web_low.svg" border=0 height="25" onmouseover="this.src='wiki_image_sm_web_high.svg'" onmouseout="this.src='wiki_image_sm_web_low.svg'">
 
</a>
 
</a>
 
</td>
 
</td>
  
 
</tr></table>
 
</tr></table>
 +
 
</td></table>
 
</td></table>
</td>
 
<td width="40%">
 
</td>
 
</tr>
 
</table>
 
</body>
 
</td></tr>
 
<tr><td height="25" bgcolor=#ffffff></td><td class="no-padding" bgcolor=#ffffff height="25" width="960" align=center>
 
<hr>
 
<table height=25 cellpadding=0 cellspacing=2 border=0><tr>
 
  
<td bgcolor=#ffffff height="25" width="90" align=center valign=center>
+
<!-- Footer End -->
<a href="https://www.eurofinsgenomics.eu/" target="_blank1" ><img src="https://static.igem.org/mediawiki/2017/6/65/T--Munich--Logo_Eurofins.png" border=0 height="15"></a>
+
</td>
+
  
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
 
  
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
 
<a href="https://www.neb.com/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/8/87/T--Munich--wiki_image_sponsors_neb.svg" border=0 height="21"></a>
 
</td>
 
  
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
</td>
 
+
<td width="40%">
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
</td>
<a href="https://www.biomers.net" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/a/a9/T--Munich--wiki_image_sponsors_biomers.png" border=0 height="21"></a>
+
</tr>
</td>
+
</table>
 
+
</body>
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
</html>
 
+
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
<a href="https://www.gatc-biotech.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/5/59/T--Munich--wiki_image_sponsors_gatc.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="70" align=center valign=center>
+
<a href="https://www3.gehealthcare.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/1/1b/T--Munich--wiki_image_sponsors_gehealthcare.svg" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="85" align=center valign=center>
+
<a href="https://eu.idtdna.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/1/1a/T--Munich--wiki_image_sponsors_idt.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="60" align=center valign=center>
+
<a href="https://www.nano-initiative-munich.de/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/a/a7/T--Munich--wiki_image_sponsors_nim.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="120" align=center valign=center>
+
<a href="https://www.promega.de/" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/3/3f/T--Munich--wiki_image_sponsors_promega.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="40" align=center valign=center>
+
<a href="https://www.qiagen.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/9/99/T--Munich--wiki_image_sponsors_qiagen.svg" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="40" align=center valign=center>
+
<a href="https://www.carlroth.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/d/d6/T--Munich--wiki_image_sponsors_roth.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="130" align=center valign=center>
+
<a href="https://www.scienova.com" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/3/3b/T--Munich--wiki_image_sponsors_scienova.png" border=0 height="21"></a>
+
</td>
+
 
+
<td bgcolor=#ffffff height="25" width="10" align=center valign=center></td>
+
 
+
<td bgcolor=#ffffff height="25" width="120" align=center valign=center>
+
<a href="https://www.unternehmertum.de" target="_blank2" ><img src="https://static.igem.org/mediawiki/2017/b/b2/T--Munich--wiki_image_sponsors_unternehmertum.png" border=0 height="21"></a>
+
</td>
+
 
+
</tr></table>
+
 
+
 
+
</td><td height="25" bgcolor=#ffffff></td></tr>
+
</table></body></html>
+

Revision as of 14:20, 13 October 2017


Date

Abstract

The ongoing crisis of increasing antibiotic resistance demands innovative preventive strategies. Recently, the RNA-targeting protein CRISPR-Cas13a has been used for highly sensitive DNA and RNA detection, promising diverse applications in point-of-care diagnostics. We integrated Cas13a in the detection unit of CascAID, our GMO-free diagnostic platform. CascAID combines an automated microfluidic device for rapid lysis and extraction of nucleic acids with a paper-based readout system. We demonstrated the performance of our device by targeting the 16S RNA from E. coli. We improved the detection limit of our platform, using simulations to optimize our amplification scheme and the final readout.

Conceived as a distributable platform for rapid point-of-care diagnostics, CascAID can be used to distinguish between bacterial and viral infections, thus minimizing the widespread use of antibiotics.

Furthermore, Cas13a allows the fast design of target sequences, making our system adaptive to the emergence of new viral outbreaks or fast mutating pathogens.

Authors
Date

Headline

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Authors
Date

Headline

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Authors
Date

Headline

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Authors