Difference between revisions of "Team:NJU-China/Attributions"

 
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<html lang="en">
 
<html lang="en">
 
<head>
 
<head>
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    <meta charset="UTF-8">
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<meta charset="UTF-8">
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    <title>PROJECT</title>
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<title>ATTRIBUTIONS</title>
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<link href="http://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css" rel="stylesheet">
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         <div class="overlay"></div>
 
         <div class="overlay"></div>
 
      
 
      
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        <!-- Sidebar -->
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      <!-- Sidebar -->
 
         <nav class="navbar navbar-inverse navbar-fixed-top" id="sidebar-wrapper" role="navigation">
 
         <nav class="navbar navbar-inverse navbar-fixed-top" id="sidebar-wrapper" role="navigation">
 
             <ul class="nav sidebar-nav">
 
             <ul class="nav sidebar-nav">
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                     <li class="dropdown-header"><a href="https://2017.igem.org/Team:NJU-China/Results/Conclusions">Results</a></li>
 
                     <li class="dropdown-header"><a href="https://2017.igem.org/Team:NJU-China/Results/Conclusions">Results</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Results/Conclusions">Conclusions</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Results/Conclusions">Conclusions</a></li>
−
                    <li><a href="https://2017.igem.org/Team:NJU-China/Demonstrate">Demonstrate</a></li>
 
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Safety">Safety</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Safety">Safety</a></li>
−
                    <li><a href="https://2017.igem.org/Team:NJU-China/Improve">Improve</a></li>
 
 
                     </ul>  
 
                     </ul>  
 
                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
 
                     <a href="https://2017.igem.org/Team:NJU-China/Basic_Part"><i class="fa fa-fw fa-plus"></i> Parts </a>  
 
                     <a href="https://2017.igem.org/Team:NJU-China/Basic_Part"><i class="fa fa-fw fa-plus"></i> Parts </a>  
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                 </li>               <li class="dropdown">
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                 </li>
 +
                <li>
 +
                    <a href="https://2017.igem.org/Team:NJU-China/Model"><i class="fa fa-fw fa-plus"></i> Model </a>
 +
                </li>                               
 +
                <li class="dropdown">
 
                   <a href="https://2017.igem.org/Team:NJU-China/Notebook" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-fw fa-plus"></i> Notebook <span class="caret"></span></a>
 
                   <a href="https://2017.igem.org/Team:NJU-China/Notebook" class="dropdown-toggle" data-toggle="dropdown"><i class="fa fa-fw fa-plus"></i> Notebook <span class="caret"></span></a>
 
                   <ul class="dropdown-menu" role="menu">
 
                   <ul class="dropdown-menu" role="menu">
 
                     <li class="dropdown-header"><a href="https://2017.igem.org/Team:NJU-China/Notebook">Notebook</a></li>
 
                     <li class="dropdown-header"><a href="https://2017.igem.org/Team:NJU-China/Notebook">Notebook</a></li>
−
                    <li><a href="https://2017.igem.org/Team:NJU-China/Experiments">Experiments</a></li>
 
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Protocol">Protocol</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/Protocol">Protocol</a></li>
−
                     <li><a href="https://2017.igem.org/Team:NJU-China/Calendar">Calendar</a></li>
+
                     <li><a href="https://2017.igem.org/Team:NJU-China/Notebook">Calendar</a></li>
 
                   </ul>
 
                   </ul>
 
                 </li>
 
                 </li>
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                     <li><a href="https://2017.igem.org/Team:NJU-China/HP/Silver">Silver HP</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/HP/Silver">Silver HP</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/HP/Gold_Integrated">Intergrated and Gold</a></li>
 
                     <li><a href="https://2017.igem.org/Team:NJU-China/HP/Gold_Integrated">Intergrated and Gold</a></li>
−
                    <li><a href="https://2017.igem.org/Team:NJU-China/Part_Collection">Public Engagement</a></li>
 
 
                   </ul>
 
                   </ul>
 
                 </li>
 
                 </li>
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             <ul>
 
             <ul>
 
                 <li>
 
                 <li>
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                     <a href="#BCL2" data-number="1">
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                     <a href="#Team" data-number="1">
 
                         <span class="cd-dot"></span>
 
                         <span class="cd-dot"></span>
−
                         <span class="cd-label">BCL2</span>
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                         <span class="cd-label">Team</span>
 
                     </a>
 
                     </a>
 
                 </li>
 
                 </li>
 
                 <li>
 
                 <li>
−
                     <a href="#tPEP" data-number="2">
+
                     <a href="#Instructors" data-number="2">
 
                         <span class="cd-dot"></span>
 
                         <span class="cd-dot"></span>
−
                         <span class="cd-label">tPEP</span>
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                         <span class="cd-label">Instructors</span>
 +
                    </a>
 +
                </li>
 +
                <li>
 +
                    <a href="#Acknowledgement" data-number="3">
 +
                        <span class="cd-dot"></span>
 +
                        <span class="cd-label">Acknowledgement</span>
 
                     </a>
 
                     </a>
 
                 </li>
 
                 </li>
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         </nav>         
 
         </nav>         
 
         <!-- Page Content -->
 
         <!-- Page Content -->
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<div id="page-content-wrapper">
 
<div id="page-content-wrapper">
 
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           <button type="button" class="hamburger is-closed animated fadeInLeft" data-toggle="offcanvas">
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             <span class="hamb-bottom"></span>
 
             <span class="hamb-bottom"></span>
 
           </button>
 
           </button>
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         <div class="teamtop">
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         </div>
 
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         <div class="page_container">
 
         <div class="page_container">
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         <div id="Team" class="first" style="padding: 50px 0;">
 
         <div id="page_edge1">
 
         <div id="page_edge1">
 
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         </div>
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             <h2>BCL2- siRNAs design</h2>
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             <h2>Team</h2>
 
             <div class="container">
 
             <div class="container">
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                <div class="row">
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            <div class="row teamwindow" style="text-align: center;">
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                        BCL2-si RNA – 1 sequence: CCGGGAGAACAGGGTATGATA
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Team leader: Zhou Yu
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                        <br />
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            <br />
−
BCL2-si RNA – 2 sequence: TACGAGTGGGATGCTGGAGAT
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Team members: Gui Yijun, Liang Chenxi, Liu Hengxin, Li Kaiming, Luo Qi, Sun Xuedi, Wu Jincheng, Zhang Zhihui
−
<br />
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            <p></p>
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BCL2-si RNA – 3 sequence: GTGGATGACTGAGTACCTGAA
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−
<br />
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            </div>
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BCL2-si RNA – 4 sequence: CTGCATCACTCTGGGTGCATA
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                </div>
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            <div class="row teamwindow" >
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                <div class="row">
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                <div class="col-md-offset-1 col-md-10">
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                    These four parts are artificially designed RNA strands that function as the silencing tool for BCL2 gene’s expression through base-pairing with corresponding mRNAs. BCL2 protein is anti-apoptotic, mainly located in the cytoplasm side of the nuclear membrane, the endoplasmic reticulum and the outer membrane of mitochondria. By muting BCL2 gene’s expression, we can induce more cells’ apoptosis theoretically. We design four sequences and have a DNA synthesis company produce plasmids with codes inserted for each of them.                  
+
                    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
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                </div>                                                          
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                        <div class="panel panel-default">
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            </div>  
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                            <div class="panel-heading" role="tab" id="headingOne">
 +
                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseOne" aria-expanded="true" aria-controls="collapseOne">
 +
                                        Leading the team
 +
                                    </a>
 +
                                </h4>
 +
                            </div>
 +
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 +
                                <div class="panel-body">
 +
                                    <p>Zhou Yu founded the team and recruited the members. He allocated assignments to us  based on our skills and interests. There is no doubt that his outstanding leadership ensured everything proceeded according to plan.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
 +
                    </div>
 +
                </div>
 +
            </div>
 +
 
 +
<div class="row teamwindow">
 +
                <div class="col-md-offset-1 col-md-10">
 +
                    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
                        <div class="panel panel-default">
 +
                            <div class="panel-heading" role="tab" id="headingTwo">
 +
                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseTwo" aria-expanded="true" aria-controls="collapseTwo">
 +
                                        Experiment
 +
                                    </a>
 +
                                </h4>
 +
                            </div>
 +
                            <div id="collapseTwo" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingTwo">
 +
                                <div class="panel-body">
 +
                                    <p>Every one of us has participated in experiments.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
 +
                    </div>
 +
                </div>
 +
            </div>
 +
 
 +
<div class="row teamwindow">
 +
                <div class="col-md-offset-1 col-md-10">
 +
                    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
                        <div class="panel panel-default">
 +
                            <div class="panel-heading" role="tab" id="headingThree">
 +
                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseThree" aria-expanded="true" aria-controls="collapseThree">
 +
                                        Wiki
 +
                                    </a>
 +
                                </h4>
 +
                            </div>
 +
                            <div id="collapseThree" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingThree">
 +
                                <div class="panel-body">
 +
                                    <p>Liu Hengxin worked on the establishment and the art design of the website. Luo Qi, Zhang Zhihui, Li Kaiming, Gui Yijun and Wu Jincheng participated in web copywriting.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
 +
                    </div>
 +
                </div>
 +
            </div>
 +
 
 +
<div class="row teamwindow">
 +
                <div class="col-md-offset-1 col-md-10">
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                    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
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                        <div class="panel panel-default">
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                            <div class="panel-heading" role="tab" id="headingFour">
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                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFour" aria-expanded="true" aria-controls="collapseFour">
 +
                                        Poster
 +
                                    </a>
 +
                                </h4>
 +
                            </div>
 +
                            <div id="collapseFour" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingFour">
 +
                                <div class="panel-body">
 +
                                    <p>Liang Chenxi, Luo Qi and Gui Yijun was responsible for writing the texts in our poster, yet Liu Hengxin and Liang Chenxi were in charge of the design of it. They cooperated very well.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
 +
                    </div>
 +
                </div>
 +
            </div>
 +
 
 +
 
 +
<div class="row teamwindow">
 +
                <div class="col-md-offset-1 col-md-10">
 +
                    <div class="panel-group" id="accordion" role="tablist" aria-multiselectable="true">
 +
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                            <div class="panel-heading" role="tab" id="headingFive">
 +
                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseFive" aria-expanded="true" aria-controls="collapseFive">
 +
                                        Human practice
 +
                                    </a>
 +
                                </h4>
 +
                            </div>
 +
                            <div id="collapseFive" class="panel-collapse collapse in" role="tabpanel" aria-labelledby="headingFive">
 +
                                <div class="panel-body">
 +
                                    <p>Gui Yijun, Liang Chenxi, Wu Jincheng, Zhou Yu and our instructor Chen Xi all went to the interview in the hospital with professionals. Liu Hengxin analyzed the data online obtained through web-crawling technique to dig into people’s attitude towards weight-losing. All of us made questionaires and distributed them out. Gui Yijun, Liang Chenxi, Luo Qi and Zhou Yu went to the CCiC held by Fujian Agriculture and Foresty University, where they got acquainted with other teams’ projects and learned a lot.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
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                    </div>
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            </div>
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 +
<div class="row teamwindow">
 +
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 +
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 +
                            <div class="panel-heading" role="tab" id="headingSix">
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                                <h4 class="panel-title">
 +
                                    <a role="button" data-toggle="collapse" data-parent="#accordion" href="#collapseSix" aria-expanded="true" aria-controls="collapseSix">
 +
                                        Presentation
 +
                                    </a>
 +
                                </h4>
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                            </div>
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 +
                                <div class="panel-body">
 +
                                    <p>Liang Chenxi, Luo Qi and Gui Yijun made the speech PPT and will give the presentations during the competition altogether.
 +
                                    </p>
 +
                                </div>
 +
                            </div>
 +
                        </div>                 
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                    </div>
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 +
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 +
        </div>
 
         </div>
 
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         <div id="tPEP" class="second" style="padding: 50px 0;">
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             <h2>tPEP - lamp 2b fusion protein’s coding DNA fragment</h2>
+
             <h2>Instructors</h2>
 
             <div class="container">
 
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            <div class="row">
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                    tPEP is a peptide (CKGGRAKDC ) targeted to adipose tissue as the ligand of adipocytes’ prohibitin, whilst lamp 2b is a integrin for exosomes. This part is artificially designed to create a fusion protein, tPep at the outward end of lamp 2b. With the assistance of such proteins, exosomes are capable of carrying siRNAs precisely towards adipose tissues. When the tPep binds the prohibitin of the adipocyte and an exosome’ membrane merge into the adipocyte’s, it’s time for siRNAs to act.                
+
            Zhou Yu : He founded the team and recruited the members. He allocated assignments to us everyone based on our strengths and interests. There is no doubt that his outstanding leadership ensured everything that needed to be done would get done.
−
                </div>
+
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                    <img src="https://static.igem.org/mediawiki/2017/c/cc/NJU_China_iGEM_2017_parts_fig1.png" alt="" style="text-align: center;" style="width:130%;">                  
+
            <h2>PI</h2>
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            <div class="container">
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                    We package BCL2-siRNAs into exosomes by transfecting HEK293 cells with a plasmid expressing BCL2-siRNA and then collect siRNA-encapsulated exosomes. When we inject the modified exosomes into the bloodstream, exosomes will specifically identify adipocytes and fuse with them under the direction of the tPep. Once siRNAs make their entrance into adipocytes, they cause the degradation of BCL2 mRNA via base-paring. Subsequently, adipocytes’ apoptosis increase.  
+
            Chen Xi : He is an amazing and inspiring teacher. Thanks to his encouragement, we are eager to put forward innovative ideas and voice our own opinions about the ongoing work. Besides, what impresses us most is that he helps us patiently whenever we have trouble with our projects. He offers so much support and help, from the construction of the whole experimental frame to the references we choose. And I believe that’s why our project went well. <br /> <br />
−
                    <br />
+
Chenyu Zhang : Professor Zhang is well-known for his research and development work about microRNAs. He provided us with experimental instruments and equipment. What’s more, he gave the guidance on the human practice and had contacted relevant authorities to guarantee all the activities in the plan could be on the rail. All of us feel very grateful for that.
−
                    To ensure the interference efficacy of BCL2 siRNA, four siRNA sequences targeting different sites of BCL2 mRNA were designed. After we transfected them into the mouse adipocytes, we could detect efficient knock-down of BCL2. Among these four sequences, BCL2-siRNA-2 exhibits the most powerful efficacy.
+
 
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                    <img src="https://static.igem.org/mediawiki/2017/1/11/NJU_China_iGEM_2017_parts_fig2.png" alt="" style="width: 50%;">
+
            <h2>Advisors</h2>
−
                    <img src="https://static.igem.org/mediawiki/2017/a/a8/NJU_China_iGEM_2017_parts_fig3.png" alt=""style="width: 50%;">
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            Zhou Zhen , Hu Xiuting
−
                    To prove the targeting property of tPep exosomes, we co-incubated them respectively with 3T3L1 cells (mouse adipose tissues) and C2C12 (mouse myoblasts). Also we set parallel experiments using exosomes without tPep-lamp 2b fusion protein. It was thrilling to see only tPep exosomes accumulated in the 3T3L1 cells while the other groups’ results were contrary. This fully illustrates tPep exosomes can target precisely to the adipose tissues.
+
            </div>
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        <div  id="Acknowledgement" class="third" style="padding: 50px 0;">
−
                    <img src="https://static.igem.org/mediawiki/2017/e/e4/NJU_China_iGEM_2017_parts_fig4.png" alt="" style="width: 50%;">
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−
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            </div>
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            <h2> Acknowledgement </h2>
−
    </div>
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<div class="container">
 +
            <div class="row">
 +
            It has been a long way for NJU-China from organizing the team to writing this. We would like to express our most sincere gratitude to every individual and institute that had helped us in the past year.
 +
            <br />
 +
Thank Prof. Zhang Chenyu and Prof. Chen Xi for their unwavering support who helped the revision of the idea. Thank our advisors Hu Xiuting and Zhou Zhen for their assistance on our experiments. Thank our instructor Zhou Yu for his invaluable input on the whole project.
 +
<br />
 +
Thanks for the funding support from M3 lab, Department of Life Science and Office of Academic Affairs of Nanjing University, who provided the laboratory and required material involved in the experiments of our project. Thank NJU Advanced Institution for Life Science and Jiangsu Engineering Center for MicroRNA Biology and Technology for the technical support. Thank Nanjing Genaral Hospital for providing the opportunity of human practice.
 +
            </div>
 +
            </div>            
 +
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      <div id="NJU-CHINA" class="foot" style="padding: 10px 0;background-color: black;color: white;">   
 
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                         <h2 style="float: left;font-size: 35px;padding: 0 0 13px 100px;font-family: 'Microsoft YaHei';letter-spacing: 1.5px;">NJU-CHINA</h2>
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                        </svg> TEAM </a>
 
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                         <a href="https://2017.igem.org/Team:NJU-China/Collaborations" class="set_1_btn Vbtn-1" style="float: right;;margin: 10px 150px 0;"> <svg>
 
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                         </svg> READ MORE </a>  
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                         </svg> COLABORATIONS </a>  
 
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Latest revision as of 02:16, 2 November 2017

ATTRIBUTIONS

Team

Team leader: Zhou Yu
Team members: Gui Yijun, Liang Chenxi, Liu Hengxin, Li Kaiming, Luo Qi, Sun Xuedi, Wu Jincheng, Zhang Zhihui

Zhou Yu founded the team and recruited the members. He allocated assignments to us based on our skills and interests. There is no doubt that his outstanding leadership ensured everything proceeded according to plan.

Every one of us has participated in experiments.

Liu Hengxin worked on the establishment and the art design of the website. Luo Qi, Zhang Zhihui, Li Kaiming, Gui Yijun and Wu Jincheng participated in web copywriting.

Liang Chenxi, Luo Qi and Gui Yijun was responsible for writing the texts in our poster, yet Liu Hengxin and Liang Chenxi were in charge of the design of it. They cooperated very well.

Gui Yijun, Liang Chenxi, Wu Jincheng, Zhou Yu and our instructor Chen Xi all went to the interview in the hospital with professionals. Liu Hengxin analyzed the data online obtained through web-crawling technique to dig into people’s attitude towards weight-losing. All of us made questionaires and distributed them out. Gui Yijun, Liang Chenxi, Luo Qi and Zhou Yu went to the CCiC held by Fujian Agriculture and Foresty University, where they got acquainted with other teams’ projects and learned a lot.

Liang Chenxi, Luo Qi and Gui Yijun made the speech PPT and will give the presentations during the competition altogether.

Instructors

Zhou Yu : He founded the team and recruited the members. He allocated assignments to us everyone based on our strengths and interests. There is no doubt that his outstanding leadership ensured everything that needed to be done would get done.

PI

Chen Xi : He is an amazing and inspiring teacher. Thanks to his encouragement, we are eager to put forward innovative ideas and voice our own opinions about the ongoing work. Besides, what impresses us most is that he helps us patiently whenever we have trouble with our projects. He offers so much support and help, from the construction of the whole experimental frame to the references we choose. And I believe that’s why our project went well.

Chenyu Zhang : Professor Zhang is well-known for his research and development work about microRNAs. He provided us with experimental instruments and equipment. What’s more, he gave the guidance on the human practice and had contacted relevant authorities to guarantee all the activities in the plan could be on the rail. All of us feel very grateful for that.

Advisors

Zhou Zhen , Hu Xiuting

Acknowledgement

It has been a long way for NJU-China from organizing the team to writing this. We would like to express our most sincere gratitude to every individual and institute that had helped us in the past year.
Thank Prof. Zhang Chenyu and Prof. Chen Xi for their unwavering support who helped the revision of the idea. Thank our advisors Hu Xiuting and Zhou Zhen for their assistance on our experiments. Thank our instructor Zhou Yu for his invaluable input on the whole project.
Thanks for the funding support from M3 lab, Department of Life Science and Office of Academic Affairs of Nanjing University, who provided the laboratory and required material involved in the experiments of our project. Thank NJU Advanced Institution for Life Science and Jiangsu Engineering Center for MicroRNA Biology and Technology for the technical support. Thank Nanjing Genaral Hospital for providing the opportunity of human practice.