Team:NJU-China/Collaborations
Collaborations
These four parts are artificially designed RNA strands that function as the silencing tool for BCL2 gene’s expression through base-pairing with corresponding mRNAs. BCL2 protein is anti-apoptotic, mainly located in the cytoplasm side of the nuclear membrane, the endoplasmic reticulum and the outer membrane of mitochondria. By muting BCL2 gene’s expression, we can induce more cells’ apoptosis theoretically. We design four sequences and have a DNA synthesis company produce plasmids with codes inserted for each of them.
SYSU-Software Team
As we showed our concern to people’s attitude towards obesity, the SYSU-Software helped us record emoji netizens would use when netizens talk about weight-related topics through web-crawling. Their work provided us with interesting data about netizens’ true reaction to weight-loss and enabled us to cater to ordinaries’ need.
CPU_China
CPU_China were very willing to help us, and they designed two other BCL2 siRNA (si-RNA 3 and 4 ) after we designed two.
BCL2-si RNA – 1 sequence: CCGGGAGAACAGGGTATGATA
BCL2-si RNA – 2 sequence: TACGAGTGGGATGCTGGAGAT
BCL2-si RNA – 3 sequence: GTGGATGACTGAGTACCTGAA
BCL2-si RNA – 4 sequence: CTGCATCACTCTGGGTGCATA
BCL2-si RNA – 2 sequence: TACGAGTGGGATGCTGGAGAT
BCL2-si RNA – 3 sequence: GTGGATGACTGAGTACCTGAA
BCL2-si RNA – 4 sequence: CTGCATCACTCTGGGTGCATA
our siRNA ( 1&2 is designed by us and 3&4 is designed by them)
NUDT_China
As soon as our siRNAs have been designed, we sent the sequence of them to NUDT_China. They synthesized the siRNA for us and tested the gene silencing effect of these four sequence. The result showed it is the BCL2 siRNA-2 that has the best effect.
NJU-CHINA
