Difference between revisions of "Team:NKU China"
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.introduction{ | .introduction{ | ||
Revision as of 08:04, 29 October 2017
INTRODUCTION
DESCRIPTION
In the process of oil recovering by water injection method, it will form large water channels in the bottom. Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. Our project is aiming to engineer Enterobacter sp. FY-07 (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose. Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out. Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.
Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria.
PROCESS ACHIEVED
1. We have cloned rhamnolipid synthesis gene (rhIABC) in Pseudomonas aeruginosa, and transformed it into Escherichia coli BL21 and successfully achieved the expression of rhIABC in E.coli BL21.
2. We have transformed rhIABC into FY-07 and successfully fulfilled the co-production of rhamnolipid and cellulose.
3. Based on E.coli fimbriae control system (E.coli fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into BL21 and proved to be feasible.
FUTURE PLAN
1. Finish the construction of plasmid.
2. Introduce molecular switch and rhamnolipid synthase into BscA gene knock out FY-07 strain.
3. Achieve controllable expression of rhamnolipid and cellulose in FY-07 strain.
4. Introduce suicide system and ensure the controllability.
COMMUNICATION AND CORPORATIONS
2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations.
3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you!
