Difference between revisions of "Team:NKU China"

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{{NKU_China/header}}
 
 
<html>
 
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            <meta charset="utf-8">
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            <meta name="viewport" content="width=device-width, initial-scale=1">
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    <div class="homefirstpage">       
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        </div>
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             <script src="https://2017.igem.org/Team:NKU_China/Javascript/bootsnav?action=raw&ctype=text/javascript"></script>      
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         <style>
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             <img class="homeplaybutton" src="https://static.igem.org/mediawiki/2017/7/73/T--NKU_China--playbutton1.png"/>
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                #top_menu_14 {
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    </div>           
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                }
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        <div style="width:100%; padding-left:100px; padding-right:100px;" class="introduction">
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             <div class="header">INTRODUCTION</div>
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            <div class="line"><img src="https://static.igem.org/mediawiki/2017/b/b8/T--NKU_China--line.png"/></div>
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             <div class="sub-header">DESCRIPTION</div>
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                    height: 16px;
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            In the process of oil recovering by water injection method, it will form large water channels in the bottom. <br/><br/>
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                }
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Water mainly flows through these channels, which stops brushing the oil hidden in the minor channel branches in soil, so that the remaining oil will be trapped into the branches. </br>
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Our project is aiming to engineer Enterobacter sp. <i>FY-07</i> (which is separated from oilfield produced water and able to produce cellulose naturally), so as to controllably produce rhamnolipid and cellulose.</br>
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                #sideMenu, #top_title {
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Rhamnolipid is a biosurfactant with the ability of emulsifying oil, so the oil can be easily washed out.
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                    display: none;
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Cellulose can clog large water channels, helping water enter into the gaps of soil and wash out the oil within them.</br><br/>
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                }
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Our project is aimed at improving oil recovery rate in the oil harvesting process using this kind of bacteria.
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             <br/><br/>
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            <div class="sub-header">PROCESS ACHIEVED</div>
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                }
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            1. We have cloned rhamnolipid synthesis gene (<i>rhIABC</i>) in Pseudomonas aeruginosa, and transformed it into <i>Escherichia coli BL21</i> and successfully achieved the expression of <i>rhIABC</i> in <i>E.coli BL21</i>.
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            <br/><br/>
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                #content {
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            2. We have transformed rhIABC into <i>FY-07</i> and successfully fulfilled the co-production of rhamnolipid and cellulose.
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            <br/><br/>
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             3. Based on <i>E.coli</i> fimbriae control system (<i>E.coli</i> fimbriae (Fim) phase variation system), we have finished the switch design, to whose both ends GFP and RFP have been linked. This modified switch has been transformed into <i>BL21</i> and proved to be feasible.
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            <br/><br/>
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                }
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            <div class="sub-header">FUTURE PLAN</div>
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            1. Finish the construction of plasmid.
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        @font-face {
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            <br/><br/>
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            font-family: 'Hotpizza';
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            2. Introduce molecular switch and rhamnolipid synthase into <i>BscA</i> gene knock out <i>FY-07</i> strain.
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            <br/><br/>
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            3. Achieve controllable expression of rhamnolipid and cellulose in <i>FY-07</i> strain.
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            4. Introduce suicide system and ensure the controllability.
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            font-weight: normal;
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            <br/><br/>
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            font-style: normal;}
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            <div class="header">COMMUNICATION AND CORPORATIONS</div>
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        @font-face {
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            1. We expect to communicate with the practitioners in oil industry so as to know more about applying petroleum microorganisms into oil production. Please contact us if you are a practitioner in oil industry who has some knowledge in this field.
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             <br/><br/>
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            2. We are looking for a suitable suicide system so that the bacteria will not survive in the environment for a long time. Please contact us if you have some recommendations.
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            <br/><br/>
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            3. Please do not hesitate to contact us if you are interested in our project. We are looking forward to communicating with you!
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            <br/><br/>
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         </div>
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</html>
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{{NKU_China/footer}}
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Revision as of 08:06, 29 October 2017