Difference between revisions of "Team:NTU SINGAPORE/Basic Part"

(Prototype team page)
 
 
(21 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
<html>
 
<html>
  
−
<div class="column full_size judges-will-not-evaluate">
+
<style>
−
<h3>★  ALERT! </h3>
+
div.ex {
−
<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
+
    height: 100%;
−
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
+
    margin: auto;
−
</div>
+
max-width:80%;
−
<div class="clear"></div>
+
text-align: center;
 +
background-color: white;
 +
}
 +
</style>
  
−
<div class="column full_width">
 
  
  
−
<h1>Basic Parts</h1>
 
  
−
<p>
 
−
A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
 
−
</p>
 
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
+
<div class="ex w3-container w3-padding-32" id="Basic Part">
−
<br>
+
    <h3 class="w3-border-bottom w3-border-light-grey w3-padding-16">Basic Part</h3>
−
<h3>Best Basic Part Special Prize</h3>
+
    <pstyle="text-align:center"> Functional units of DNA.</p>
 +
  </div>
 +
<div id='groupparts' style='min-height:100px;width:1084px;margin:auto;'><div style='width:300px;margin:auto;padding:20px;color:gray;border:1px solid gray'>Loading…..</div></div><script>$('#groupparts').load('/cgi/api/groupparts.cgi', { t:'iGEM2017', g:'NTU_Singapore' },function(){ $('#groupparts .tablesorter').tablesorter();} );</script>
  
−
<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are *many* opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2017.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
 
  
−
<br><br>
+
<div class="column full_width">
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
+
−
<br>
+
  
 +
<div class="ex w3-container w3-padding-32" id="∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced">
 +
    <h3 class="w3-border-bottom w3-border-light-grey w3-padding-16">∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced</h3>
 +
    <pstyle="text-align:center"> Validated Part </p>
 +
  </div>
  
  
−
<div class="highlight">
+
<div class="ex column full_width">
−
<h4>Note</h4>
+
−
<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
+
  
 +
<p>dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner.</p>
  
 +
<p>For this part, we have deleted the HNH domain, Rec2 domain and RuvCIII-2 domain of the SP-dCas9. The triple truncation (∆REC2 ∆RuvCIII-2 ∆HNH, referred to as ‘∆3ple’) is especially of interest since it reduces the size of the dCas9 gene to around 3.2kB which is on par with the size of Sa-Cas9. The ∆3ple truncation has slightly lower activation compared to double truncation. In order to improve the activity of ∆3ple dCas9, quick changes are further performed at the various site of dCas9. Combination of quick change at Asn497Lys (QC5), Thr657Lys (QC6) and Ser1109Arg (QC15) show improved activity. Its binding capability was tested by assessing the strength in transcriptional activation via by tagging it with a VP64-p65-Rta tripartite activator.</p>
 
</div>
 
</div>
 +
 +
 +
</br>
 +
 +
<div class="ex column full_size" >
 +
<img src="https://static.igem.org/mediawiki/2017/3/30/Part1ntu.jpg" >
 +
</br>
 +
</br>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2017/c/cf/Parts_2ntu.jpg" >
 +
</br>
 +
</br>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2017/b/ba/Parts3ntu.jpg" >
 +
</br>
 +
</br>
 +
</br>
 +
<img src="https://static.igem.org/mediawiki/2017/b/ba/Parts4ntu.jpg" >
 +
</br>
 +
</br>
 +
</br>
 +
</div>
 +
</div>
 +
</div>
 +
 +
  
  
 
</html>
 
</html>

Latest revision as of 02:55, 2 November 2017



HOME
Our Team Collaboration
Description Experiment Proof of Concept Demonstrate Improve
INTERLAB STUDY
Basic Parts
Our Story Silver Gold
ATTRIBUTIONS CONTACT US

Basic Part

Functional units of DNA.

Loading…..

∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced

Validated Part

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner.

For this part, we have deleted the HNH domain, Rec2 domain and RuvCIII-2 domain of the SP-dCas9. The triple truncation (∆REC2 ∆RuvCIII-2 ∆HNH, referred to as ‘∆3ple’) is especially of interest since it reduces the size of the dCas9 gene to around 3.2kB which is on par with the size of Sa-Cas9. The ∆3ple truncation has slightly lower activation compared to double truncation. In order to improve the activity of ∆3ple dCas9, quick changes are further performed at the various site of dCas9. Combination of quick change at Asn497Lys (QC5), Thr657Lys (QC6) and Ser1109Arg (QC15) show improved activity. Its binding capability was tested by assessing the strength in transcriptional activation via by tagging it with a VP64-p65-Rta tripartite activator.