Line 42: | Line 42: | ||
<p>Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.</p> | <p>Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.</p> | ||
− | <p> | + | <p>IPTG and 20mM of glucose was used to induce the gene expression. Comparison of 1mM IPTG and 20mM IPTG was made and it was found that 1mM IPTG has higher gene expression level. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products.</p> |
Revision as of 04:16, 25 October 2017