Difference between revisions of "Team:NTU SINGAPORE/Collaborations"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h2> Macquaire iGEM Team </h2>
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<p>Macquaire team had constructed a hydrogenase gene capable of converting glucose to hydrogen gas. The plasmid (BBa_KXXXXXX) that consist of hydrogenase enzyme (Hyd1), ferredoxin, ferredoxin-NADPH-reductase (FNR) and maturation enzyme (HydEF and HydG), in which their expression are co-regulated under pLac promoter.</p>
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<p>This year again, our team helped them to evaluate each of the gene expression level by using RT-PCR.</p>
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<p>The results of RT-PCR is shown as below: </p>
  
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<h1>Collaborations</h1>
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<p> Formula: 2^-(ΔΔCt) is used to calculate the fold change of gene expression after induction, in which
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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ΔCt(control)1= Ct(uninduced operon genes)- Ct(Chloramphenicol gene)ΔCt(test)2 = Ct(Induced operon genes)-Ct(Induced Chloramphenicol gene)</p>
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<p>Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.</p>
  
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<h3>Silver Medal Criterion #2</h3>
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<p>As shown from the above bar charts, 20mM of IPTG induction has greater fold change of gene expression than 200mM of IPTG induction. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products.</p>
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Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2017.igem.org/Judging/Medals">2017 Medals Page</a> for more information.  
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<h4> Which other teams can we work with? </h4>
 
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
 
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
 
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Here are some suggestions for projects you could work on with other teams:
 
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<li> Improve the function of another team's BioBrick Part or Device</li>
 
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<li> Characterize another team's part </li>
 
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<li> Debug a construct </li>
 
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<li> Model or simulating another team's system </li>
 
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<li> Test another team's software</li>
 
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<li> Help build and test another team's hardware project</li>
 
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<li> Mentor a high-school team</li>
 
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Revision as of 18:44, 22 October 2017



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Macquaire iGEM Team


Macquaire team had constructed a hydrogenase gene capable of converting glucose to hydrogen gas. The plasmid (BBa_KXXXXXX) that consist of hydrogenase enzyme (Hyd1), ferredoxin, ferredoxin-NADPH-reductase (FNR) and maturation enzyme (HydEF and HydG), in which their expression are co-regulated under pLac promoter.


This year again, our team helped them to evaluate each of the gene expression level by using RT-PCR.


The results of RT-PCR is shown as below:

Formula: 2^-(ΔΔCt) is used to calculate the fold change of gene expression after induction, in which ΔCt(control)1= Ct(uninduced operon genes)- Ct(Chloramphenicol gene)ΔCt(test)2 = Ct(Induced operon genes)-Ct(Induced Chloramphenicol gene)


Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.

As shown from the above bar charts, 20mM of IPTG induction has greater fold change of gene expression than 200mM of IPTG induction. It is worth to notice that the RT-PCR efficiency might be different for different genes due to their size of PCR products and the formula used above is assuming the PCR efficiency to be 100% that eventually resulted in 2 copies of products.