Kasturi001 (Talk | contribs) |
Kasturi001 (Talk | contribs) |
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<p> Formula: | <p> Formula: | ||
− | </br>2^-(ΔΔCt) is used to calculate the fold change of gene expression after induction, in which | + | </br>2^-(ΔΔCt) is used to calculate the fold change of gene expression after induction, in which</p> |
− | ΔCt(control)1= Ct(uninduced operon genes)- Ct(Chloramphenicol gene) | + | <p>ΔCt(control)1= Ct(uninduced operon genes)- Ct(Chloramphenicol gene)</p> |
</br> | </br> | ||
− | ΔCt(test)2 = Ct(Induced operon genes)-Ct(Induced Chloramphenicol gene) | + | <p>ΔCt(test)2 = Ct(Induced operon genes)-Ct(Induced Chloramphenicol gene)</p> |
</br> | </br> | ||
Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.</p> | Finally, use ΔCt(test)2- ΔCt(control)1 to get ΔΔCt.</p> |
Revision as of 12:45, 24 October 2017