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=Protocol= | =Protocol= | ||
+ | |||
+ | Our Lab's Protocols | ||
+ | |||
+ | == Medium == | ||
+ | |||
+ | === LB medium (100 mL liquid) === | ||
+ | |||
+ | 1.Measure 1g Tripton | ||
+ | |||
+ | 2.Measure 0.5g Yeast Extract | ||
+ | |||
+ | 3.Measure 1g Nacl | ||
+ | |||
+ | 4.Add 100mL H2O | ||
+ | |||
+ | 5.autoclave(121℃ 20min) | ||
+ | |||
+ | |||
+ | |||
+ | == DNA work == | ||
+ | |||
+ | |||
+ | === Agarose gel(100mL) === | ||
+ | |||
+ | Method of Making 0.7% Agarose gel | ||
+ | |||
+ | 1.Measure 0.7g Agarose | ||
+ | |||
+ | 2.Add 100mL TAE buffer | ||
+ | |||
+ | 3.Heat(till agarose melted)*We used a microwave oven. | ||
+ | |||
+ | 4.Pur agarose into a gel maker | ||
+ | |||
+ | 5.Set a comb | ||
+ | |||
+ | 6.Wait till agarose curdles | ||
+ | |||
+ | 7.Pull an comb |
Revision as of 18:33, 1 November 2017
Nagahama
Contents
Protocol
Our Lab's Protocols
Medium
LB medium (100 mL liquid)
1.Measure 1g Tripton
2.Measure 0.5g Yeast Extract
3.Measure 1g Nacl
4.Add 100mL H2O
5.autoclave(121℃ 20min)
DNA work
Agarose gel(100mL)
Method of Making 0.7% Agarose gel
1.Measure 0.7g Agarose
2.Add 100mL TAE buffer
3.Heat(till agarose melted)*We used a microwave oven.
4.Pur agarose into a gel maker
5.Set a comb
6.Wait till agarose curdles
7.Pull an comb