Difference between revisions of "Team:Newcastle/Protocols Jack"

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          <h1>Protocols</h1>
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          <p>Archive of all protocols used by team members<br />
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            The SOP template can be downloaded <a href="https://static.igem.org/mediawiki/2017/b/b0/T--Newcastle--sop_template.docx">here</a>.</p>
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      <!-- Title -->
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                <h1>Protocols - Click headers to sort:</h1>
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      <div class="row">
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          <div class="col-lg-12">
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              <h3Protocols- click the headers to sort:</h3>
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      <div class="row text-center">
 
  
 
<table class="sortable wikitable">
 
<table class="sortable wikitable">
 +
  <tr>
 +
    <th onmouseover="" style="cursor: pointer;">Protocol</th>
 +
    <th onmouseover="" style="cursor: pointer;">Author</th>
 +
    <th onmouseover="" style="cursor: pointer;">Category</th>
 +
    <th></th>
 +
  </tr>
 +
  <tr>
 +
    <td><i>E .coli</i> Transformation</td>
 +
    <td>Bradley Brown</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/1/1f/T--Newcastle--ecoli_transformation_bb.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>Bacterial CFPS Cell Extract Preparation Procedure</td>
 +
    <td>Bradley Brown</td>
 +
    <td>Cell Free Protein Synthesis</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Newcastle--BB_protocol_bacterial_cell_extract.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>CFPS Amino Acid Mix Preparation</td>
 +
    <td>Bradley Brown</td>
 +
    <td>Cell Free Protein Synthesis</td>
 +
    <td><a href="" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>Competent <i>E .coli</i> Cells</td>
 +
    <td>Lais Takiguchi</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/7/7b/T--Newcastle--ecoli_competent_cells_lct.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>Fim Switch 96 well plate protocol</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>Fim Switch 96 well plate reader assay</td>
 +
    <td><a href="https://static.igem.org/mediawiki/parts/b/b1/--T--Newcastle--MP--Protocol--Fim--96.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>gBlock and HiFi Assembly</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/3/38/T--Newcastle--gBlock-HiFi.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>Agarose gel electrophoresis</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/a/a3/T--Newcastle--DNA_Extraction.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>DNA Gel Extraction</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/e/e1/T--Newcastle--gel.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>Miniprep</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/e/e1/T--Newcastle--Miniprep.pdf" style="color:black">View</a></td>
 +
  </tr>
 +
  <tr>
 +
    <td>DNA Restriction Digest</td>
 +
    <td>Marcia Pryce</td>
 +
    <td>General</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2017/1/13/T--Newcastle--digest.pdf" style="color:black">View</a></td>
 +
  </tr>
 
<tr>
 
<tr>
−
  <th onmouseover="" style="cursor: pointer;">Protocol</th>
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    <td>Glycerol Stock</td>
−
  <th onmouseover="" style="cursor: pointer;">Author</th>
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    <td>Marcia Pryce</td>
−
  <th onmouseover="" style="cursor: pointer;">Category</th>
+
    <td>General</td>
−
  <th></th>
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    <td><a href="https://static.igem.org/mediawiki/2017/7/77/T--Newcastle--stock.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td><i>E .coli</i> Transformation</td>
+
    <td>Overnight Culture</td>
−
  <td>Bradley Brown</td>
+
    <td>Marcia Pryce</td>
−
  <td>General</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/1/1f/T--Newcastle--ecoli_transformation_bb.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/7/73/T--Newcastle--cultures.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>Bacterial CFPS Cell Extract Preparation Procedure</td>
+
    <td>T4 Blunting</td>
−
  <td>Bradley Brown</td>
+
    <td>Marcia Pryce</td>
−
  <td>Cell Free Protein Synthesis</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/e/e6/T--Newcastle--BB_protocol_bacterial_cell_extract.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/2/2b/T--Newcastle--T4-Blunt.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>CFPS Amino Acid Mix Preparation</td>
+
    <td>Agarose Gel</td>
−
  <td>Bradley Brown</td>
+
    <td>Marcia Pryce</td>
−
  <td>Cell Free Protein Synthesis</td>
+
    <td>General</td>
−
  <td><a href="" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/d/d1/T--Newcastle--agarose.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>Competent <i>E .coli</i> Cells</td>
+
    <td>Agar Plate</td>
−
  <td>Lais Takiguchi</td>
+
    <td>Marcia Pryce</td>
−
  <td>General</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/7/7b/T--Newcastle--ecoli_competent_cells_lct.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/b/b6/T--Newcastle--agar_plate.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>Fim Switch 96 well plate protocol</td>
+
    <td>LB Broth Recipe</td>
−
  <td>Marcia Pryce</td>
+
    <td>Marcia Pryce</td>
−
  <td>Fim Switch 96 well plate reader assay</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/parts/b/b1/--T--Newcastle--MP--Protocol--Fim--96.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/5/58/T--Newcastle--LB_Broth.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>gBlock and HiFi Assembly</td>
+
    <td>Ligation</td>
−
  <td>Marcia Pryce</td>
+
    <td>Marcia Pryce</td>
−
  <td>General</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/3/38/T--Newcastle--gBlock-HiFi.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/6/6d/T--Newcastle--Ligase.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>Agarose gel electrophoresis</td>
+
    <td>Q5 PCR</td>
−
  <td>Marcia Pryce</td>
+
    <td>Marcia Pryce</td>
−
  <td>General</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/a/a3/T--Newcastle--DNA_Extraction.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/8/8a/T--Newcastle--Q5_PCR.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
 
<tr>
 
<tr>
−
  <td>DNA Gel Extraction</td>
+
    <td>Taq Colony PCR</td>
−
  <td>Marcia Pryce</td>
+
    <td>Marcia Pryce</td>
−
  <td>General</td>
+
    <td>General</td>
−
  <td><a href="https://static.igem.org/mediawiki/2017/e/e1/T--Newcastle--gel.pdf" style="color:black">View</a></td>
+
    <td><a href="https://static.igem.org/mediawiki/2017/e/e3/T--Newcastle--Taq_PCR.pdf" style="color:black">View</a></td>
−
</tr>
+
  </tr>
−
<tr>
+
−
  <td>Miniprep</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/e/e1/T--Newcastle--Miniprep.pdf" style="color:black">View</a></td>
+
−
</tr>
+
−
<tr>
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−
  <td>DNA Restriction Digest</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/1/13/T--Newcastle--digest.pdf" style="color:black">View</a></td>
+
−
</tr>
+
−
<tr>
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−
  <td>Glycerol Stock</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/7/77/T--Newcastle--stock.pdf" style="color:black">View</a></td>
+
−
</tr>
+
−
<tr>
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−
  <td>Overnight Culture</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/7/73/T--Newcastle--cultures.pdf" style="color:black">View</a></td>
+
−
</tr>
+
−
<tr>
+
−
  <td>T4 Blunting</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/2/2b/T--Newcastle--T4-Blunt.pdf" style="color:black">View</a></td>
+
−
</tr>
+
−
<tr>
+
−
  <td>Agarose Gel</td>
+
−
  <td>Marcia Pryce</td>
+
−
  <td>General</td>
+
−
  <td><a href="https://static.igem.org/mediawiki/2017/d/d1/T--Newcastle--agarose.pdf" style="color:black">View</a></td>
+
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Revision as of 15:03, 1 November 2017

spacefill

Protocols - Click headers to sort:

Protocol Author Category
E .coli Transformation Bradley Brown General View
Bacterial CFPS Cell Extract Preparation Procedure Bradley Brown Cell Free Protein Synthesis View
CFPS Amino Acid Mix Preparation Bradley Brown Cell Free Protein Synthesis View
Competent E .coli Cells Lais Takiguchi General View
Fim Switch 96 well plate protocol Marcia Pryce Fim Switch 96 well plate reader assay View
gBlock and HiFi Assembly Marcia Pryce General View
Agarose gel electrophoresis Marcia Pryce General View
DNA Gel Extraction Marcia Pryce General View
Miniprep Marcia Pryce General View
DNA Restriction Digest Marcia Pryce General View
Glycerol Stock Marcia Pryce General View
Overnight Culture Marcia Pryce General View
T4 Blunting Marcia Pryce General View
Agarose Gel Marcia Pryce General View
Agar Plate Marcia Pryce General View
LB Broth Recipe Marcia Pryce General View
Ligation Marcia Pryce General View
Q5 PCR Marcia Pryce General View
Taq Colony PCR Marcia Pryce General View