Difference between revisions of "Team:Newcastle/Results"

Line 595: Line 595:
 
           <img src="https://static.igem.org/mediawiki/2017/b/b1/Vava1aa.png" class="img-fluid border border-dark rounded" style="margin: 2%; max-width: 70%">
 
           <img src="https://static.igem.org/mediawiki/2017/b/b1/Vava1aa.png" class="img-fluid border border-dark rounded" style="margin: 2%; max-width: 70%">
 
<p>
 
<p>
<center><b>Figure 1:</b> <a href="http://parts.igem.org/Part:BBa_J33201">BBa_J33201</a> design.</center>
+
<center><b>Figure 1:</b> <a href="http://parts.igem.org/Part:BBa_J33201">BBa_J33201</a> design.</center></p>
 
+
</p>
+
  
 
           <p>This part consists of the promoter of the  <i> E. coli</i>  JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the <i>arsR</i> gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.</p>
 
           <p>This part consists of the promoter of the  <i> E. coli</i>  JM109 chromosomal arsenic detoxification operon (ars operon), including the ArsR repressor binding site and the <i>arsR</i> gene encoding the arsR repressor protein, together with its ribosome binding site. Addition of any other genes to the 3' end of this part will result in their expression being dependent on the presence of sodium arsenate or sodium arsenite. Arsenite or arsenite anion binds to the repressor protein ArsR, resulting in inability to repress the promoter. Based on our experiments, a concentration of 1 micromolar sodium arsenate in LB is sufficient for essentially full expression, though this will vary according to conditions.</p>

Revision as of 15:12, 1 November 2017

spacefill

Our Experimental Results


Below is a diagram of our Sensynova Framework. Clicking on each part of the framework (e.g. detector modules) links to the relevant results.

Alternatively, at the bottom of this page are tabs which will show you results for every part of the project



Framework

Framework Chassis

Biochemical Adaptor

Target

Detector Modules

Multicellular Framework Testing

C12 HSL: Connector 1

Processor Modules

Framework in Cell Free Protein Synthesis Systems

C4 HSL: Connector 2

Reporter Modules



Looking for Interlab Study
related results? Click below!