Difference between revisions of "Team:Newcastle/Results"

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           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> Characterisation </h2>
 
           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> Characterisation </h2>
           <p><b>Qualitative assay.</b> Due to time constraints only a preliminary qualitative assay was carried out. Co-cultures of Arsenic detector, processor unit and 3 different reporter modules carrying 2 chromoproteins (<a href="http://parts.igem.org/Part:BBa_K2205016">BBa_K2205016</a>, <a href="http://parts.igem.org/Part:BBa_K2205018">BBa_K2205018</a>) and sfGFP (<a href="http://parts.igem.org/Part:BBa_K2205015">BBa_K2205015</a>) were inoculated and grown overnight in LB+chloramphenicol (12.5ng/ul). The day after the cultures were diluted at OD600: 0,1 and mixed together to obtain co-cultures with ratio 1:1:13 (detector:processor:reporter). The samples were supplemented with different concentration of Arsenic (0ppb, 10ppb, 50ppb, 100ppb) to induce the expression of quorum sensing molecules and eventually achieve the chromoproteins visualisation (Figures 6, 7, 8). </p>
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           <p><b>Qualitative assay.</b> Due to time constraints only a preliminary qualitative assay was carried out. Co-cultures of Arsenic detector, processor unit and 3 different reporter modules carrying 2 chromoproteins (<a href="http://parts.igem.org/Part:BBa_K2205016">BBa_K2205016</a>, <a href="http://parts.igem.org/Part:BBa_K2205018">BBa_K2205018</a>) and sfGFP (<a href="http://parts.igem.org/Part:BBa_K2205015">BBa_K2205015</a>) were inoculated and grown overnight in LB+chloramphenicol (12.5ng/ul). The day after the cultures were diluted at OD600 0.1 and mixed together to obtain co-cultures with ratio 1:1:13 (detector:processor:reporter). The samples were supplemented with different concentration of Arsenic (0ppb, 10ppb, 50ppb, 100ppb) to induce the expression of quorum sensing molecules and eventually achieve the chromoproteins visualisation (Figures 6, 7, 8). </p>
 
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Revision as of 15:43, 1 November 2017

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Our Experimental Results


Below is a diagram of our Sensynova Framework. Clicking on each part of the framework (e.g. detector modules) links to the relevant results.

Alternatively, at the bottom of this page are tabs which will show you results for every part of the project



Framework

Framework Chassis

Biochemical Adaptor

Target

Detector Modules

Multicellular Framework Testing

C12 HSL: Connector 1

Processor Modules

Framework in Cell Free Protein Synthesis Systems

C4 HSL: Connector 2

Reporter Modules



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