Difference between revisions of "Team:Newcastle/Results"

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           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> Design Stage </h2>
 
           <h2 style="font-family: Rubik; text-align: left; margin-top: 1%"> Design Stage </h2>
 
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<p>This entire construct was flanked by 30 bp overhangs with the pSB1C3 plasmid, so that it could be Gibson assembled into a pSB1C3 plasmid digested with XbaI and SpeI. Extra bases were added between the overhangs and the construct so that once the part was assembled into the plasmid, the XbaI and SpeI sites could be regenerated and the biobrick prefix and suffix restored. A T7 promoter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
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<p>This construct was designed to have 30 bp overhangs with the pSB1C3 plasmid, so that it could be Gibson assembled into a pSB1C3 plasmid digested with XbaI and SpeI. Extra bases were added between the overhangs and the construct so that once the part was assembled into the plasmid, the XbaI and SpeI sites could be regenerated and the biobrick prefix and suffix restored. A T7 promoter was added to enable expression to be under the control of IPTG. This construct was then submitted to IDT for synthesis as a gBlock.
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To ensure the codon usage of our SOX protein was not differing significantly from the average codon usage of  <i> E. coli</i>, rare codons were removed from the sequence using the <a href="https://www.idtdna.com/CodonOpt">IDT codon optimisation tool</a>to produce high protein expression.</p>
 
To ensure the codon usage of our SOX protein was not differing significantly from the average codon usage of  <i> E. coli</i>, rare codons were removed from the sequence using the <a href="https://www.idtdna.com/CodonOpt">IDT codon optimisation tool</a>to produce high protein expression.</p>
 
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Revision as of 18:01, 1 November 2017

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Our Experimental Results


Below is a diagram of our Sensynova Framework. Clicking on each part of the framework (e.g. detector modules) links to the relevant results.

Alternatively, at the bottom of this page are tabs which will show you results for every part of the project



Framework

Framework Chassis

Biochemical Adaptor

Target

Detector Modules

Multicellular Framework Testing

C12 HSL: Connector 1

Processor Modules

Framework in Cell Free Protein Synthesis Systems

C4 HSL: Connector 2

Reporter Modules



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related results? Click below!