Difference between revisions of "Team:Northwestern/Lab"

 
(35 intermediate revisions by 2 users not shown)
Line 40: Line 40:
  
 
</head>  
 
</head>  
 
<style>
 
 
</style>
 
 
   
 
   
 
<body>  
 
<body>  
Line 50: Line 46:
  
 
<center><h1 style="padding-top:2%; padding-right: 15%; padding-left:15%"><br><br>Lab Notebook</h3></center>
 
<center><h1 style="padding-top:2%; padding-right: 15%; padding-left:15%"><br><br>Lab Notebook</h3></center>
<h3 class="w3-center"> Click headings to collapse/expand the menu </h3>
 
  
 
<br>
 
<br>
  
 
<div id="nestedAccordion" class="w3-center">
 
<div id="nestedAccordion" class="w3-center">
   <button class="w3-button w3-block"> Weekly Summaries</button>
+
   <button class="w3-button w3-block panel-collapse collapse" style="font-size:30px; padding-top: 2%; padding-bottom: 2%"> Weekly summaries</button>
     <div>
+
     <div style="display:none;">
         <h3 border:0> Week 1/2 </h3>
+
         <h3 class="w3-button w3-block"> Week 1 and 2</h3>
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/archive/3/3d/20170802184027%21T--Northwestern--Week2.pdf" type="application/pdf" width="75%" height="600px">
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/archive/3/3d/20170802184027%21T--Northwestern--Week2.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/archive/3/3d/20170802184027%21T--Northwestern--Week2.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/archive/3/3d/20170802184027%21T--Northwestern--Week2.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
         <h3>Week 3</h3>
+
         <h3 class="w3-button w3-block"> Week 3</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/d/d9/T--Northwestern--Week3.pdf" type="application/pdf" width="75%" height="600px">
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/d/d9/T--Northwestern--Week3.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/d/d9/T--Northwestern--Week3.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/d/d9/T--Northwestern--Week3.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
         <h3>Week 4</h3>
+
         <h3 class="w3-button w3-block"> Week 4</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/6/66/T--Northwestern--Week4.pdf" type="application/pdf" width="75%" height="600px">
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/6/66/T--Northwestern--Week4.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/6/66/T--Northwestern--Week4.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/6/66/T--Northwestern--Week4.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
           <h3>Week 5</h3>
+
           <h3 class="w3-button w3-block"> Week 5</h3 >
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/1/16/Lab_Notebook_-_Week_5_%287-10-17-7-16-17%29_2017-07-10_-_2017-07-14_%28etr_XTi9rICh%29.pdf" type="application/pdf" width="75%" height="600px">
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/1/16/Lab_Notebook_-_Week_5_%287-10-17-7-16-17%29_2017-07-10_-_2017-07-14_%28etr_XTi9rICh%29.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/1/16/Lab_Notebook_-_Week_5_%287-10-17-7-16-17%29_2017-07-10_-_2017-07-14_%28etr_XTi9rICh%29.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/1/16/Lab_Notebook_-_Week_5_%287-10-17-7-16-17%29_2017-07-10_-_2017-07-14_%28etr_XTi9rICh%29.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
           <h3>Week 6</h3>
+
           <h3 class="w3-button w3-block"> Week 6</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/e/ee/T--Northwestern--Week6.pdf" type="application/pdf" width="75%" height="600px">
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/e/ee/T--Northwestern--Week6.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/e/ee/T--Northwestern--Week6.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/e/ee/T--Northwestern--Week6.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
           <h3>Week 7</h3>
+
           <h3 class="w3-button w3-block"> Week 7</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b9/T--Northwestern--Week7.pdf" type="application/pdf" width="75%" height="600px">
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b9/T--Northwestern--Week7.pdf" type="application/pdf" width="75%" height="600px">
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/b/b9/T--Northwestern--Week7.pdf"></a>
 
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/b/b9/T--Northwestern--Week7.pdf"></a>
 +
</object> </center> </div>
 +
        <h3 class="w3-button w3-block"> Week 8-9</h3 >
 +
        <div> <center><object data="https://static.igem.org/mediawiki/2017/7/72/T-Northwestern--Week8.pdf" type="application/pdf" width="75%" height="600px">
 +
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/7/72/T-Northwestern--Week8.pdf"></a>
 +
</object> </center> </div>
 +
 +
        <h3 class="w3-button w3-block"> Week 10</h3 >
 +
        <div> <center><object data="https://static.igem.org/mediawiki/2017/d/d7/T-Northwestern--Week10.pdf" type="application/pdf" width="75%" height="600px">
 +
Your device does not support embed PDFs. Please click the following link to open up the PDF. <a href="https://static.igem.org/mediawiki/2017/d/d7/T-Northwestern--Week10.pdf"></a>
 
</object> </center> </div>
 
</object> </center> </div>
  
 
     </div>
 
     </div>
  
  <button class="w3-button w3-block"> OMV Fusion Project </button>
+
  <button class="w3-button w3-block" style="font-size : 30px; padding-top: 2%; padding-bottom: 2%"> Protocols </button>
     <div> </div>  
+
     <div style="display:none;">  
 
+
    <h3 id = "input" class="w3-button w3-block"> Making TAE buffer</h3 >  
<button class="w3-button w3-block"> Protocols  </button>
+
    <div>
+
    <h3> Making TAE Buffer </h3>  
+
 
<div> <center><object data="https://static.igem.org/mediawiki/2017/c/ca/T--Northwestern--10xTAE.pdf
 
<div> <center><object data="https://static.igem.org/mediawiki/2017/c/ca/T--Northwestern--10xTAE.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 96: Line 97:
 
</object> </center> </div>  
 
</object> </center> </div>  
  
     <h3> Culture Innoculation </h3>  
+
     <h3 class="w3-button w3-block"> Culture Innoculation</h3 >
 
       <div>  <center><object data="https://static.igem.org/mediawiki/2017/7/72/T--Northwestern--CultureInoculation.pdf
 
       <div>  <center><object data="https://static.igem.org/mediawiki/2017/7/72/T--Northwestern--CultureInoculation.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 103: Line 104:
 
</object> </center> </div>  
 
</object> </center> </div>  
  
     <h3> Gemstone Dye Protocol </h3>  
+
     <h3 class="w3-button w3-block"> Gemstone dye protocol</h3 >  
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f7/T--Northwestern--GemstoneDye.pdf
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f7/T--Northwestern--GemstoneDye.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 111: Line 112:
  
  
       <h3> Fractionation </h3>  
+
       <h3 class="w3-button w3-block"> Fractionation</h3 >
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/1/18/T--Northwestern--Fractionation.pdf
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/1/18/T--Northwestern--Fractionation.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 118: Line 119:
 
</object> </center>  </div>  
 
</object> </center>  </div>  
 
      
 
      
       <h3> LB + Plate </h3>  
+
       <h3 class="w3-button w3-block"> LB + Plate</h3 >
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/d/da/T--Northwestern--LBPlate.pdf
 
       <div> <center><object data="https://static.igem.org/mediawiki/2017/d/da/T--Northwestern--LBPlate.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 125: Line 126:
 
</object> </center> </div>  
 
</object> </center> </div>  
  
       <h3> Supplememnted Media </h3>  
+
       <h3 class="w3-button w3-block"> Supplemented Media</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/7/73/T--Northwestern--M9SupplementedMedia.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/7/73/T--Northwestern--M9SupplementedMedia.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 132: Line 133:
 
</object> </center> </div>  
 
</object> </center> </div>  
 
          
 
          
         <h3> Transformations </h3>  
+
         <h3 class="w3-button w3-block"> Transformations</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f8/T--Northwestern--TransformationProtocol.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/f/f8/T--Northwestern--TransformationProtocol.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 139: Line 140:
 
</object> </center> </div>
 
</object> </center> </div>
 
          
 
          
           <h3> Miniprep (Promega) </h3>  
+
           <h3 class="w3-button w3-block"> Miniprep (Promega)</h3 >  
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/9/9b/T--Northwestern--MiniprepPromega.pdf
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/9/9b/T--Northwestern--MiniprepPromega.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 146: Line 147:
 
</object> </center> </div>  
 
</object> </center> </div>  
 
          
 
          
           <h3> OMV Purification </h3>
+
           <h3 class="w3-button w3-block"> OMV Purification</h3 >
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/e/e0/T--Northwestern--OMVPurification.pdf
 
           <div> <center><object data="https://static.igem.org/mediawiki/2017/e/e0/T--Northwestern--OMVPurification.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 152: Line 153:
 
"></a>
 
"></a>
 
</object> </center> </div>  
 
</object> </center> </div>  
 
+
<h3 class="w3-button w3-block">PCR for Removal of His6 Tag from DsbA</h3 >
            <h3> PCR for Removal of His6 Tag from DsbA </h3>  
+
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/9/91/T--Northwestern--PCRHisRemoval.pdf
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/9/91/T--Northwestern--PCRHisRemoval.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 160: Line 160:
 
</object> </center> </div>  
 
</object> </center> </div>  
  
             <h3> Sequencing </h3>  
+
             <h3 class="w3-button w3-block">Sequencing</h3 >
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/7/71/T--Northwestern--Sequencing.pdf
 
             <div> <center><object data="https://static.igem.org/mediawiki/2017/7/71/T--Northwestern--Sequencing.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 167: Line 167:
 
</object> </center> </div>  
 
</object> </center> </div>  
  
             <h3> 2xYT </h3>  
+
             <h3 class="w3-button w3-block">2xYT</h3 >
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/5/54/T--Northwestern--RecipeFor2xYT.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/5/54/T--Northwestern--RecipeFor2xYT.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 174: Line 174:
 
</object> </center> </div>  
 
</object> </center> </div>  
 
            
 
            
             <h3> Self-circularization of Linear DNA </h3>  
+
             <h3 class="w3-button w3-block">Self-circularization of linear DNA</h3 >  
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b3/T--Northwestern--SelfCircularization.pdf
 
         <div> <center><object data="https://static.igem.org/mediawiki/2017/b/b3/T--Northwestern--SelfCircularization.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 181: Line 181:
 
</object> </center> </div>
 
</object> </center> </div>
  
             <h3> Spectinmyocin </h3>  
+
             <h3 class="w3-button w3-block">Spectinmyocin</h3 >
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/0/09/T--Northwestern--Spectinomycin.pdf
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/0/09/T--Northwestern--Spectinomycin.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 187: Line 187:
 
"></a>
 
"></a>
 
</object> </center> </div>
 
</object> </center> </div>
              <h3> Tyler's Transformation and Fluorescence </h3>  
+
            <h3 class="w3-button w3-block">Tyler's Transformation and Fluorescence</h3 >  
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/9/9c/T--Northwestern--TylerFluorescence.pdf
 
         <div>  <center><object data="https://static.igem.org/mediawiki/2017/9/9c/T--Northwestern--TylerFluorescence.pdf
 
" type="application/pdf" width="75%" height="600px">
 
" type="application/pdf" width="75%" height="600px">
Line 197: Line 197:
 
</div>  
 
</div>  
 
          
 
          
 
 
  
 
</div>
 
</div>
Line 207: Line 205:
  
 
</body>  
 
</body>  
 
 
  
 
</html>
 
</html>
{{Northwestern_Page_Foot_temp}}
 

Latest revision as of 03:55, 2 November 2017

Northwestern Template Northwestern Template

Northwestern Template



Lab Notebook


Week 1 and 2

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 3

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 4

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 5

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 6

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 7

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 8-9

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Week 10

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Making TAE buffer

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Culture Innoculation

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Gemstone dye protocol

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Fractionation

Your device does not support embed PDFs. Please click the following link to open up the PDF.

LB + Plate

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Supplemented Media

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Transformations

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Miniprep (Promega)

Your device does not support embed PDFs. Please click the following link to open up the PDF.

OMV Purification

Your device does not support embed PDFs. Please click the following link to open up the PDF.

PCR for Removal of His6 Tag from DsbA

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Sequencing

Your device does not support embed PDFs. Please click the following link to open up the PDF.

2xYT

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Self-circularization of linear DNA

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Spectinmyocin

Your device does not support embed PDFs. Please click the following link to open up the PDF.

Tyler's Transformation and Fluorescence

Your device does not support embed PDFs. Please click the following link to open up the PDF.