Team:Oxford/Software

Software Tool

Enter the disease name, parasite or specific protease to find the cleavage sequence of protease specific to the disease of interest and how our system may be applicable.

Disease Congenital Chagas Disease Parasite Trypanosoma Cruzi Specific Protease Cruzipain DNA Sequence of Cleavage Site AAAAAAGTTAAAGCTAAAAAA Protease Cleavage Sequence K/K/L/KR-A/KS/K/RQ Medium of Test Blood Final Output Hirudin Positive Result No blood clotting

Disease Sepsis Parasite Pseudomonas aeruginosa Specific Protease LasA DNA Sequence of Cleavage Site Protease Cleavage Sequence After Gly-Gly (e.g. PGVGG - elastin) or between Lys-Ile (e.g. NKKIEKFQ(S-P) - beta=casein) Medium of Test Blood Final Output Hirudin Positive Result No blood clotting

Disease African Sleeping sickness Parasite Trypanosoma brucei Specific Protease Rhodesain/Brucepain DNA Sequence of Cleavage Site TCCGCGCCGAAATCCCTGATTGGC/ TCCGCGCCGCGTTCCCTGATTGGC Protease Cleavage Sequence S/A/P/R-K/S/L/I/G Medium of Test Blood Final Output Hirudin Positive Result No blood clotting

Disease Schistosomiasis Parasite Schistosoma mansoni Specific Protease Cercarial elastase DNA Sequence of Cleavage Site Cercarial elastase Protease Cleavage Sequence AGCTGGCCGCTG Medium of Test SWPL Final Output Hirudin Positive Result No blood clotting

Disease Congenital toxoplasmosis Parasite Toxoplasma gondii Specific Protease Cathepsin L DNA Sequence of Cleavage Site AAACAGAAACTGCGC Protease Cleavage Sequence KQKLR Medium of Test Amniotic fluid Final Output Luciferase Positive Result Light

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