Description

This project involves the modification of the bacterium Escherichia coli, through Synthetic Biology, which is based on the creation or modification of existing organisms, making them have the characteristics that we want. This organism will sense the high levels of alpha, beta, gamma rays and X-rays emitted, factors that cause a mutation in the DNA of said bacterium that will give a purple coloration and the aroma to lemon. To validate that the research is necessary and supported by specialists in the area, a survey was conducted to radiology students, medical technologists and radiology center workers, obtaining an 83% approval, on the need for better dosimeters that are reliable results .

The sensor and its parts

For this through a scientific investigation, a sensor was designed that is based on a biphasic switch, device that can be turned off or on according to the concentration of the imput. This switch naturally regulates lambda phage, which in this case when activated the Promoter pRM gives way to the transcription of the Cl repressor, which in turn will cause inhibition of the Pr promoter and can not be transcribed Cro, LuxR and LuxI. In contrast, if DNA damage is detected with the help of the RecA protein, the Cl repressor is removed and the pRM promoter is stopped, giving rise to pR (promoter) activation and to the transcription of Cro, LuxR and LuxI . (Plasmid 1)