Difference between revisions of "Team:Szeged SA RMG/Description"

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<h1>Description</h1>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/members">Members</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/mentors">Mentors</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/Attributions">Attributions</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/collaboration">Collaborations</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/pictures">Gallery</a>
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<h5>Advice on writing your Project Description</h5>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/summary">Summary</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/articles">Article</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/bioinfo">Bioinformatics</a>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.  
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/presentation">Presentation</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/poster">Poster</a>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/survey">Survey</a>
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<h5>References</h5>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/methane">Methane</a>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/highschooloutreach">High School Outreach</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/universityoutreach">University Outreach</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/methods">Methods</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/labequipments">Lab Equipments</a>
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    <a href="https://2017.igem.org/Team:Szeged_SA_RMG/safety">Safety</a>
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      <ul class="nav navbar-nav navbar-right">
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        <li><a href="http:/www.methungeny.com">METHUNGENY</a></li>
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      <h1><center>Summary</center></h1>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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Methane is one of the most produced greenhouse gases, which traps heat in the atmosphere increasing global warming. Methane is produced due to industrial and agricultural activities. However, in high concentration it's also harmful for human health. Nowadays, because of increasing global warming, methane production must be regulated; therefore indicating methane gets higher priority.
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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Our aim is to develop a cheap methane biosensor consisting of modified methane-utilizing bacteria and enzymatic assay. As an energy and carbon source, they oxidize methane to methanol, methanol to formaldehyde and formaldehyde to formic acid. To our project we have chosen a well-known bacterium, Methylococcus capsulatus.
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The enzymatic assay in the medium is methanol specific, so we need to make the bacteria produce methanol. Our idea is to knock out the gene of the enzyme transforming methanol to formaldehyde, called methanol-dehydrogenase. As a result methanol is accumulated in the medium; consequently, it can be indicated by a colour change reaction performed by the enzyme. Furthermore, the colour of the medium can be easily perceived by an electric tool.       
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Our product can be used as an alternative for present methane sensors in developing countries. For instance, in mines and indoors, where high concentration of methane can be released, it can function as an alarm device to prevent explosions and potential inhaling. Besides, we can discover yet unknown methane sources such as in swamps. </p5>
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Revision as of 16:33, 1 November 2017

Summary

Methane is one of the most produced greenhouse gases, which traps heat in the atmosphere increasing global warming. Methane is produced due to industrial and agricultural activities. However, in high concentration it's also harmful for human health. Nowadays, because of increasing global warming, methane production must be regulated; therefore indicating methane gets higher priority. Our aim is to develop a cheap methane biosensor consisting of modified methane-utilizing bacteria and enzymatic assay. As an energy and carbon source, they oxidize methane to methanol, methanol to formaldehyde and formaldehyde to formic acid. To our project we have chosen a well-known bacterium, Methylococcus capsulatus. The enzymatic assay in the medium is methanol specific, so we need to make the bacteria produce methanol. Our idea is to knock out the gene of the enzyme transforming methanol to formaldehyde, called methanol-dehydrogenase. As a result methanol is accumulated in the medium; consequently, it can be indicated by a colour change reaction performed by the enzyme. Furthermore, the colour of the medium can be easily perceived by an electric tool. Our product can be used as an alternative for present methane sensors in developing countries. For instance, in mines and indoors, where high concentration of methane can be released, it can function as an alarm device to prevent explosions and potential inhaling. Besides, we can discover yet unknown methane sources such as in swamps.