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+ | <a href="https://2017.igem.org/Team:Szeged_SA_RMG/summary">Summary</a> | ||
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+ | <a href="https://2017.igem.org/Team:Szeged_SA_RMG/bioinfo">Bioinformatics</a> | ||
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+ | <a href="https://2017.igem.org/Team:Szeged_SA_RMG/presentation">Presentation</a> | ||
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+ | <a href="https://2017.igem.org/Team:Szeged_SA_RMG/highschooloutreach">High School Outreach</a> | ||
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+ | <ul class="nav navbar-nav navbar-right"> | ||
+ | <li><a href="http:/www.methungeny.com">METHUNGENY</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
− | < | + | <h1><center>MODEL</center></h1> |
− | < | + | |
− | < | + | <h3><left>The goal of the measurement</left></h3> |
+ | <left> | ||
+ | <p5 class="w3-text-grey"> | ||
+ | Our final product is a genetically modified Methylococcus capsulatus strain, which - hopefully - produce lactate from methane. In order to estimate the possible effectiveness of this bacteria, we decided to use a lactate | ||
− | </ | + | assay kit. The main idea of our final measurement is a 3D graph which will give us a clue how our product is able to measure the methane concentration effectively. </p5> |
− | + | </left> | |
− | < | + | <h3><left>The calculation of a certain data</left></h3> |
− | < | + | <left> |
− | < | + | <p5 class="w3-text-grey"> |
− | + | The measurement will be carried out with Sigma-Aldrich Colorimetric Lactate Assay Kit (MAK064). During the measurement a calibration curve should be determined and used to obtain the lactate concentration of the medium. | |
− | + | ||
− | + | Concentration of lactate in a certain medium solution, based on the protocol provided by the distributor company, can be calculated as C=Sa/Sv, where Sa = Amount of lactate acid in unknown sample (nmol) from standard curve; | |
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− | + | Sv = Sample volume (mL) added into the wells; C = Concentration of lactate acid in sample.</p5> | |
− | < | + | </left> |
− | < | + | <left> |
− | + | <p5 class="w3-text-grey"> | |
− | + | The measurement scheme is as follows:</p5> | |
− | + | </left> | |
− | </ | + | |
− | < | + | <center> <img src="https://static.igem.org/mediawiki/2017/f/fc/Methungeny_model.jpg"> </center> |
− | < | + | |
− | < | + | <left> |
− | < | + | <p5 class="w3-text-grey"> |
− | + | Unfortunately we couldn't manage to obtain the final bacteria, thus the measurement remained a plan for the next week - hopefully we can present the results at the Jamboree. </p5> | |
− | + | </left> | |
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− | </ | + | |
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</html> | </html> |
Latest revision as of 03:27, 2 November 2017