Difference between revisions of "Team:Szeged SA RMG/Model"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2017.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2017.igem.org/Judging/Awards"> award listed above</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2017.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Modeling</h1>
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<p>Mathematical models and computer simulations provide a great way to describe the function and operation of BioBrick Parts and Devices. Synthetic Biology is an engineering discipline, and part of engineering is simulation and modeling to determine the behavior of your design before you build it. Designing and simulating can be iterated many times in a computer before moving to the lab. This award is for teams who build a model of their system and use it to inform system design or simulate expected behavior in conjunction with experiments in the wetlab.</p>
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        <li><a href="http:/www.methungeny.com">METHUNGENY</a></li>
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<h1><center>MODEL</center></h1>
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<h3> Gold Medal Criterion #3</h3>
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<p>
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To complete for the gold medal criterion #3, please describe your work on this page and fill out the description on your <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. To achieve this medal criterion, you must convince the judges that your team has gained insight into your project from modeling. You may not convince the judges if your model does not have an effect on your project design or implementation.
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<h3><left>The goal of the measurement</left></h3>
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Please see the <a href="https://2017.igem.org/Judging/Medals"> 2017 Medals Page</a> for more information.
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<p5 class="w3-text-grey">  
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Our final product is a genetically modified Methylococcus capsulatus strain, which - hopefully - produce lactate from methane. In order to estimate the possible effectiveness of this bacteria, we decided to use a lactate
  
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assay kit. The main idea of our final measurement is a 3D graph which will give us a clue how our product is able to measure the methane concentration effectively. </p5>
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<h3>Best Model Special Prize</h3>
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<h3><left>The calculation of a certain data</left></h3>
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To compete for the <a href="https://2017.igem.org/Judging/Awards">Best Model prize</a>, please describe your work on this page  and also fill out the description on the <a href="https://2017.igem.org/Judging/Judging_Form">judging form</a>. Please note you can compete for both the gold medal criterion #3 and the best model prize with this page.
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You must also delete the message box on the top of this page to be eligible for the Best Model Prize.
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The measurement will be carried out with Sigma-Aldrich Colorimetric Lactate Assay Kit (MAK064). During the measurement a calibration curve should be determined and used to obtain the lactate concentration of the medium.  
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Concentration of lactate in a certain medium solution, based on the protocol provided by the distributor company, can be calculated as C=Sa/Sv, where Sa = Amount of lactate acid in unknown sample (nmol) from standard curve;
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Sv = Sample volume (mL) added into the wells; C = Concentration of lactate acid in sample.</p5>
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<h5> Inspiration </h5>
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Here are a few examples from previous teams:
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<li><a href="https://2016.igem.org/Team:Manchester/Model">Manchester 2016</a></li>
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<li><a href="https://2016.igem.org/Team:TU_Delft/Model">TU Delft 2016  </li>
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<li><a href="https://2014.igem.org/Team:ETH_Zurich/modeling/overview">ETH Zurich 2014</a></li>
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<li><a href="https://2014.igem.org/Team:Waterloo/Math_Book">Waterloo 2014</a></li>
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The measurement scheme is as follows:</p5>
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<center> <img src="https://static.igem.org/mediawiki/2017/f/fc/Methungeny_model.jpg"> </center>
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Unfortunately we couldn't manage to obtain the final bacteria, thus the measurement remained a plan for the next week - hopefully we can present the results at the Jamboree. </p5>
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Latest revision as of 03:27, 2 November 2017

MODEL

The goal of the measurement

Our final product is a genetically modified Methylococcus capsulatus strain, which - hopefully - produce lactate from methane. In order to estimate the possible effectiveness of this bacteria, we decided to use a lactate assay kit. The main idea of our final measurement is a 3D graph which will give us a clue how our product is able to measure the methane concentration effectively.

The calculation of a certain data

The measurement will be carried out with Sigma-Aldrich Colorimetric Lactate Assay Kit (MAK064). During the measurement a calibration curve should be determined and used to obtain the lactate concentration of the medium. Concentration of lactate in a certain medium solution, based on the protocol provided by the distributor company, can be calculated as C=Sa/Sv, where Sa = Amount of lactate acid in unknown sample (nmol) from standard curve; Sv = Sample volume (mL) added into the wells; C = Concentration of lactate acid in sample. The measurement scheme is as follows:
Unfortunately we couldn't manage to obtain the final bacteria, thus the measurement remained a plan for the next week - hopefully we can present the results at the Jamboree.