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Revision as of 14:37, 26 October 2017

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Basic Parts

We provide few basic parts which are used in our Detecoli, includingBBa_K2383001andBBa_K2383010.

BBa_K2383001

This chromoprotein from the ''Cnidopus japonicus'' sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in ''E coli''. We add a LVA tag after it to accelerate its degradation rate.

BBa_K2383010

LacI without LVA tag