Difference between revisions of "Team:TCFSH Taiwan/Basic Part"

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<link href="https://2016.igem.org/Team:NCTU_Formosa/wiki_reset?action=raw&ctype=text/css" rel="stylesheet">
 
 
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<!--------LacI----------->
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<!--LacI-->
 
<a href="http://parts.igem.org/Part:BBa_K2383010" class="biobrick"><p class="content-1">BBa_K2383010</p></a>
 
<a href="http://parts.igem.org/Part:BBa_K2383010" class="biobrick"><p class="content-1">BBa_K2383010</p></a>
 
<p class="content">LacI without LVA tag</p>
 
<p class="content">LacI without LVA tag</p>
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       <img src="https://static.igem.org/mediawiki/2017/0/0d/LACInew.jpeg" class="bigphoto" width=75%;>
 
       <img src="https://static.igem.org/mediawiki/2017/0/0d/LACInew.jpeg" class="bigphoto" width=75%;>
 
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<p class="content">These two are the basic parts we use in our project.</p>
 
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Revision as of 16:30, 28 October 2017

Basic Parts

We provide few basic parts which are used in our Detecoli, includingBBa_K2383001andBBa_K2383010.

BBa_K2383001

This chromoprotein from the ''Cnidopus japonicus'' sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (BBa_K592009), amilGFP (BBa_K592010), spisPink (BBa_K1033932), asPink (BBa_K1033933) and aeBlue (BBa_K864401), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in ''E coli''. We add a LVA tag after it to accelerate its degradation rate.

BBa_K2383010

LacI without LVA tag

These two are the basic parts we use in our project.