Line 391: | Line 391: | ||
</div> | </div> | ||
− | <! | + | <!--wiki content--> |
Line 405: | Line 405: | ||
<img src="https://static.igem.org/mediawiki/2017/5/5e/Cjblue%2Blva.jpeg" class="bigphoto" width=70%;> | <img src="https://static.igem.org/mediawiki/2017/5/5e/Cjblue%2Blva.jpeg" class="bigphoto" width=70%;> | ||
− | <p class="content">This chromoprotein from the "Cnidopus japonicus" sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (<partinfo>BBa_K592009</partinfo>), amilGFP (<partinfo>BBa_K592010</partinfo>), spisPink (<partinfo>BBa_K1033932</partinfo>), asPink (<partinfo>BBa_K1033933</partinfo>) and aeBlue (<partinfo>BBa_K864401</partinfo>), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in "<span style="font-style:italic;">E. coli</span>". We add a LVA tag after protein, then proteinases will more likely yo break it down, and wull thus make it degrade faster.</p> | + | <p class="content">This chromoprotein from the "<span style="font-style:italic;">Cnidopus japonicus</span>" sea anemone, cjBlue, naturally exhibits dark green color when expressed. Compared to some other chromoproteins, such as amilCP (<partinfo>BBa_K592009</partinfo>), amilGFP (<partinfo>BBa_K592010</partinfo>), spisPink (<partinfo>BBa_K1033932</partinfo>), asPink (<partinfo>BBa_K1033933</partinfo>) and aeBlue (<partinfo>BBa_K864401</partinfo>), the color development is slower. The color is readily observed in both LB or on agar plates after 24-48 hours of incubation. The sequence is codon optimized for expression in "<span style="font-style:italic;">E. coli</span>". We add a LVA tag after protein, then proteinases will more likely yo break it down, and wull thus make it degrade faster.</p> |
Revision as of 03:07, 1 November 2017