Difference between revisions of "Team:TCFSH Taiwan/Composite Part"

Line 472: Line 472:
 
<p class="content"> We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:</p>
 
<p class="content"> We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/b/b8/Composite1.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/1/17/Brick4.jpeg" class="bigphoto" width=70%>
  
 
<p class="content-1">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
 
<p class="content-1">R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)</p>
Line 481: Line 481:
 
<p class="content">This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:</p>
 
<p class="content">This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/0/0a/Composite2.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/9/9e/Brick3.jpeg" class="bigphoto" width=70%>
  
 
<p class="content-1">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)</p>
 
<p class="content-1">J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)</p>
Line 490: Line 490:
 
<p class="content">This is a Report system of UV and Temperature with LacI. The biobrick will be like:</p>
 
<p class="content">This is a Report system of UV and Temperature with LacI. The biobrick will be like:</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/3/38/Composite3.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/4/4c/Brick6.jpeg" class="bigphoto" width=70%>
  
 
<p class="content-1">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
 
<p class="content-1">B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)</p>
Line 498: Line 498:
 
<a href="http://parts.igem.org/Part:BBa_K2383301" class="biobrick"><p class="title">Composite BBa_K2383301</p></a>
 
<a href="http://parts.igem.org/Part:BBa_K2383301" class="biobrick"><p class="title">Composite BBa_K2383301</p></a>
 
<p class="content">This is a Report system of UV with LacI. The biobrick will be like:</p>
 
<p class="content">This is a Report system of UV with LacI. The biobrick will be like:</p>
−
 
+
https://static.igem.org/mediawiki/2017/7/7a/Brick2.jpeg
−
<img src="https://static.igem.org/mediawiki/2017/a/a1/Composite4.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/7/7a/Brick2.jpeg" class="bigphoto" width=70%>
 
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + E1010 (RFP) + B0034 + BBa_K2383010</p>
 
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + E1010 (RFP) + B0034 + BBa_K2383010</p>
  
Line 507: Line 507:
 
<p class="content">This is a Report system of Temperature with LacI. The biobrick will be like:</p>
 
<p class="content">This is a Report system of Temperature with LacI. The biobrick will be like:</p>
  
−
<img src="https://static.igem.org/mediawiki/2017/e/e4/Composite5.jpeg" class="bigphoto" width=70%>
+
<img src="https://static.igem.org/mediawiki/2017/7/7a/Brick5.jpeg" class="bigphoto" width=70%>
 
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + BBa_K592009 + B0034 + BBa_K2383010 (LacI) + B0010 + B0012</p>
 
<p class="content-1">BBa_K1725400 (PlsiR) + B0034 + BBa_K592009 + B0034 + BBa_K2383010 (LacI) + B0010 + B0012</p>
  

Revision as of 01:03, 2 November 2017

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Composite Parts

We have know the function of two basic parts, the cjBlue-LVA and LacI without LVA. Hence, we create biobrick using the basic parts we mentioned and other different parts which will be mentioned below.

Composite BBa_K2383100

We us the cjBlue_LVA in this part, and add UirR, UirS into it. The biobrick will be like:

R0011 (Promoter) + B0034 (RBS) +BBa_k2383001 + B0034 (RBS) +BBa_K1725420 (UirR) + BBa_K1725410 (UirS)

Composite BBa_K2383200

This is a RhlR Regulating system with RNA thermometer. The biobrick will be like:

J23101 (Promoter) + BBa_K115002 (RBS) + BBa_K115002 + BBa_C0171 (RhlR) + BBa_K1541017 (RhlI)

Composite BBa_K2383300

This is a Report system of UV and Temperature with LacI. The biobrick will be like:

B0010 + B0012 + BBa_K2383010 (LacI) + B0034 + BBa_I14017 (Rhl) + BBa_K1725400 (PlsiR) + B0034 + E1010 + B0034 BBa_K2383010 (LacI)

Composite BBa_K2383301

This is a Report system of UV with LacI. The biobrick will be like:

https://static.igem.org/mediawiki/2017/7/7a/Brick2.jpeg

BBa_K1725400 (PlsiR) + B0034 + E1010 (RFP) + B0034 + BBa_K2383010

Composite BBa_K2383302

This is a Report system of Temperature with LacI. The biobrick will be like:

BBa_K1725400 (PlsiR) + B0034 + BBa_K592009 + B0034 + BBa_K2383010 (LacI) + B0010 + B0012

These are the composite parts we use in our project.