Difference between revisions of "Team:TCFSH Taiwan/Description"

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     <p class="title">How we design our biobrick</p>
 
     <p class="title">How we design our biobrick</p>
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     <p class="content">First, we tried to find a UV promoter, and we located<font style="color: orange">BBa_I765001</font>. However, it simply didn’t work in our experiment.</div>
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     <p class="content">First, we tried to find a UV promoter, and we located<font style="color: orange">BBa_I765001</font>. However, it simply didn’t work in our experiment. So after searching on the Internet, we found a project that had been conducted by Rice university. They found that protein UirR (<font style="color:orange">K1725420</font>) and UirS (<font style="color: orange">K1725410</font>) can be used as a photo receptor. The UirS protein is anchored in the bacterial membrane where it “sees” the color illuminating the bacterium. If the illumination is UV, UirS activates itself and releases the protein, UirR. UirR will then be phosphorylated, and become active. Active UirR is mobile, capable of binding a specific promoter called (PcsiR1), and triggering the expression of the desired gene—RFP (<font style="color: orange">E1010</font>). However, we couldn’t find the promoter sequence of PcsiR1 at first, so we used Plsir (<font style="color: orange">K1725400</font>) instead. But when we eventually found the sequence, it was too late for us. So we designed this biobrick:</p>
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    <p class="content"><font style="color: lightblue">Pcon RBS UirR RBS</font> (<font style="color: orange">B0034</font>) <font style="color: lightblue">UirS Ter Ter</font> (<font style="color: orange">B0015</font>)</p>
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    <p class="content"><font style="color: lightblue">Plsir RBS RFP Ter Ter</font></p>
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    </div>
 
</section>
 
</section>
 
</h1>
 
</h1>

Revision as of 15:18, 29 October 2017

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JUDGING FORM

Motivation

A bottle of cold drink is a fantastic idea on a scorching summer day, but what if the drink is contaminated with plasticizers? Many people know that plastic bottles will release harmful plasticizers into the drink at high temperatures, so they sensibly avoid leaving bottled drinks or water in hot places, like their cars. However, what if the drink has been contaminated during transportation from the factory to the retail store? Besides bottled beverages, many other products, such as cosmetics or medicine, may pose potential threats to human health if they are stored at inadequate temperatures or exposed to sunlight for too long during the delivery process. The danger is real and immediate, but it is humanly impossible to monitor every delivery procedure. Therefore, we plan to invent a biosensor which can help monitor and ensure the cargo quality in transit.

Overview

We transplant a modified gene into e-coli, and after drying them up, we put these special e-coli into a specialized sticker made by ourselves, and manufactured a device that can do long-term detection. The sticker monitors the temperature and UV light in the whole transportation process, and will change the color on its outlook. By simply take a glance on it, the customers can directly confirm the condition of the merchandise.(see Design & Device)

How we design our biobrick

First, we tried to find a UV promoter, and we locatedBBa_I765001. However, it simply didn’t work in our experiment. So after searching on the Internet, we found a project that had been conducted by Rice university. They found that protein UirR (K1725420) and UirS (K1725410) can be used as a photo receptor. The UirS protein is anchored in the bacterial membrane where it “sees” the color illuminating the bacterium. If the illumination is UV, UirS activates itself and releases the protein, UirR. UirR will then be phosphorylated, and become active. Active UirR is mobile, capable of binding a specific promoter called (PcsiR1), and triggering the expression of the desired gene—RFP (E1010). However, we couldn’t find the promoter sequence of PcsiR1 at first, so we used Plsir (K1725400) instead. But when we eventually found the sequence, it was too late for us. So we designed this biobrick:

Pcon RBS UirR RBS (B0034) UirS Ter Ter (B0015)

Plsir RBS RFP Ter Ter