Line 345: | Line 345: | ||
padding-bottom:10px; | padding-bottom:10px; | ||
} | } | ||
+ | |||
+ | .bigphoto{ | ||
+ | margin: auto; | ||
+ | display: block; | ||
+ | } | ||
</style> | </style> | ||
Line 436: | Line 441: | ||
<p class="content"><font style="color: lightblue">Plsir RBS RFP Ter Ter</font></p> | <p class="content"><font style="color: lightblue">Plsir RBS RFP Ter Ter</font></p> | ||
+ | |||
+ | <p class="content">Then, we needed to find a way to measure the temperature, which is using the temperature regulated RBS (<font style="color: orange">BBa_K115001</font>). This RBS only allows ribosomes to bind on it at the temperature of 37 degree Celsius or above. Originally, we decided to put GFP after it, and the GFP would be activated if it reaches the target temperature. But we then noticed that GFP would produce green light, and green light would cause the protein UirS to reverse back into an inactive state. So we then chose BFP (<font style="color: orange">K592009</font>) instead, and designed this biobrick:</p> | ||
+ | |||
+ | <p class="content"><font style="color: green">Pcon RBSTemp BFP Ter Ter</font></p> | ||
+ | |||
+ | <p class="content">For fear that our products might be damaged, causing the bacteria inside to die, we designed a mechanism to guarantee that our product will remain effective. We found that if we put an LVA tag behind the chromoprotein, it will degrade much faster. So our concept is to make the bacteria produce chromoprotein constantly, and it will be colorful when it is working. Nonetheless, when the bacteria aren’t alive anymore, the color will degrade fast and eventually become colorless. In the end, we designed this biobrick:</p> | ||
+ | |||
+ | <p class="content"><font style="color: lightblue">Pcon RBS cj-Blue-lva Ter Ter</font> <font style="color: red">(note that cj-Blue looks green)</font></p> | ||
+ | |||
+ | <p class="content">Lastly, since it would be difficult to transform more than three plasmid into the bacteria, we combined two of them with one in the reverse direction (we are afraid that the gene behind will express poorly), and try to make the sequence as short as possible. So the final biobrick is:</p> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2017/3/38/Composite3.jpeg" class="bigphoto" width="70%"> | ||
</div> | </div> | ||
</section> | </section> |
Revision as of 15:24, 29 October 2017