Difference between revisions of "Team:TCFSH Taiwan/HP/Silver"

Line 251: Line 251:
 
.title{
 
.title{
 
     font-size:25pt !important;
 
     font-size:25pt !important;
−
     color:rgb(0, 206, 255);
+
     color:black;
−
     text-shadow:1px 1px 10px rgb(0, 206, 255);
+
     text-shadow:1px 1px 10px rgb(153, 153, 255);
−
     border-left:3px solid rgb(0, 206, 255);
+
     border-left:3px solid rgb(153, 153, 255);
 
     padding-left:10px !important;   
 
     padding-left:10px !important;   
 
     margin-top:20px;
 
     margin-top:20px;
Line 363: Line 363:
 
    
 
    
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">The Problem & Solution</p></div>
+
           <div class="topic"><p class="text_color">Visiting Professor</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 376: Line 376:
 
    
 
    
 
           <div class="node">
 
           <div class="node">
−
           <div class="topic"><p class="text_color">Overview</p></div>
+
           <div class="topic"><p class="text_color">Precious Suggestions</p></div>
 
             <div class="active-circle">
 
             <div class="active-circle">
 
               <div class="splash"></div>
 
               <div class="splash"></div>
Line 415: Line 415:
 
<section class="content_container">
 
<section class="content_container">
 
   <div>
 
   <div>
−
     <p class="title">The Problem & Solution<p>
+
     <p class="title">Visiting Professor<p>
−
     <p class="content">Effective pest control has long been a pivotal issue in modern agriculture. With the escalating growth of world population and the change in diet, high levels of agricultural production are required to meet the demand for food production. To date, a vast majority of farmers uses chemical pesticide to kill the pest worldwide<sup>[1]</sup>, it is by far the most convenient and effective way of pesticide control. However, the increased production chemical pesticides brought entails multiple negative costs that are usually overlooked, sacrificing human health and the environment. Thus, problems of chemical pesticide involving food safety, farmers’ health, soil & water pollution, and the existence of drug-resistant insects are looming. An alternative solution of pest control is urgently needed, necessitating the nascence of our project.</p>
+
     <p class="content">We’ve interviewed professor Chang Cheng Ming on 10/14. Professor Chang is an expert in the transportation process for frozen food.
 +
And we had learn some precious information during the interview.</p>
 
      
 
      
  
 
      
 
      
−
     <p class="title">Overview</p>
+
     <p class="title">Precious Suggestions</p>
−
     <p class="content">We designed a nature-inspired system to offer farmers a safe, environmentally-friendly, accurate, and automatic style of farming. Inspired by the spiders using web and venom to achieve the predation, the system also contains two concept-resembling parts—“Device and PANTIDE.” As engineering, internet of things (IoT), and synthetic biology collide, the transference of knowledge to state-of-the-art system helps farmers no longer need to do laborious farm works and spray hazardous chemical poisons. It is a revolutionary system that not only guarantees the safety of the farmers and customers worldwide but also safeguards the sustainability of the environment and ecosystem. (see <a href="https://2016.igem.org/Team:NCTU_Formosa/Design" style="color:#44E287;">Design</a> & <a href="https://2016.igem.org/Team:NCTU_Formosa/Demonstrate" style="color:#44E287;">Device</a>)</p>
+
     <p class="content">We’ve interviewed professor Chang Cheng Ming on 10/14. Professor Chang is an expert in the transportation process for frozen food. During the interview, we had learned two important information. The first point is that our product is not applicable for the foods that need to be preserved in the fridge. Since the highest temperature that those foods can tolerate is 15 degrees Celsius, our product won’t be useful for them because the temperature we set is 37 degrees Celsius. The second point is the technology of temperature measuring in the low temperature is already perfect. It can be divided into 3 parts. First, if you are looking for temperature monitors with high accuracy, you can buy one for 500 dollars. It can measure the temperature every 10 or 20 seconds, and it will record 64000 information. When you need the information, just simply plug them into a computer and every single data will be clear. Second, if you are looking for something cheaper, you can purchase a card with changeable barcode on it for 3 dollars. There are various inks used to print the barcode, and each kind of them will appear under different temperature through irreversible processes. This is, the highest temperature in the transportation process would be recorded, and all you should do is use a scanner to scan the barcode, and you’ll get the information you want. Finally, if you are looking for something cheaper still, you can purchase the fresh meter sticker. It’s only 3 cents for each, and it would change color under different temperature. The main drawback of it is that it isn’t so accurate. In conclusion, our product is not adequate for frozen food, and even if we can change the temperature, it’ll still be a challenge for us to beat the current products.</p>
  
  
−
     <p class="title">Improvement of Previous BioBrick</p>
+
     <p class="title">Further Concept</p>
 
     <p class="content">This year, we have designed fusion proteins of spider toxin in conjunction with snowdrop lectin by the three-alanine linker. Through the reference searching, we found that the previous research all used the three-alanine linker. In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. So we went to the linker library in iGEM part registry website to search for a longer linker. At first, we planned to use the Flexible Peptide GS Linker(<a href="http://parts.igem.org/Part:BBa_K416001" style="color:#44E287;">BBa_K416001</a>) from 2010 NYU iGEM team. However, we found no result in the previous history using this linker. Therefore, we modified the sequence and added three more amino acids into the linker in case it was still too short for proper protein folding. In the experiment(See the <a href="https://2016.igem.org/Team:NCTU_Formosa/Proof#GS_Linker" style="color:#44E287;">Link</a>), the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.(See the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030" style="color:#44E287;">Link</a>)</p>
 
     <p class="content">This year, we have designed fusion proteins of spider toxin in conjunction with snowdrop lectin by the three-alanine linker. Through the reference searching, we found that the previous research all used the three-alanine linker. In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. So we went to the linker library in iGEM part registry website to search for a longer linker. At first, we planned to use the Flexible Peptide GS Linker(<a href="http://parts.igem.org/Part:BBa_K416001" style="color:#44E287;">BBa_K416001</a>) from 2010 NYU iGEM team. However, we found no result in the previous history using this linker. Therefore, we modified the sequence and added three more amino acids into the linker in case it was still too short for proper protein folding. In the experiment(See the <a href="https://2016.igem.org/Team:NCTU_Formosa/Proof#GS_Linker" style="color:#44E287;">Link</a>), the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.(See the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030" style="color:#44E287;">Link</a>)</p>
 
   </div>
 
   </div>

Revision as of 08:36, 26 October 2017

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
InterLab
Contribution
Model
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM

Visiting Professor

We’ve interviewed professor Chang Cheng Ming on 10/14. Professor Chang is an expert in the transportation process for frozen food. And we had learn some precious information during the interview.

Precious Suggestions

We’ve interviewed professor Chang Cheng Ming on 10/14. Professor Chang is an expert in the transportation process for frozen food. During the interview, we had learned two important information. The first point is that our product is not applicable for the foods that need to be preserved in the fridge. Since the highest temperature that those foods can tolerate is 15 degrees Celsius, our product won’t be useful for them because the temperature we set is 37 degrees Celsius. The second point is the technology of temperature measuring in the low temperature is already perfect. It can be divided into 3 parts. First, if you are looking for temperature monitors with high accuracy, you can buy one for 500 dollars. It can measure the temperature every 10 or 20 seconds, and it will record 64000 information. When you need the information, just simply plug them into a computer and every single data will be clear. Second, if you are looking for something cheaper, you can purchase a card with changeable barcode on it for 3 dollars. There are various inks used to print the barcode, and each kind of them will appear under different temperature through irreversible processes. This is, the highest temperature in the transportation process would be recorded, and all you should do is use a scanner to scan the barcode, and you’ll get the information you want. Finally, if you are looking for something cheaper still, you can purchase the fresh meter sticker. It’s only 3 cents for each, and it would change color under different temperature. The main drawback of it is that it isn’t so accurate. In conclusion, our product is not adequate for frozen food, and even if we can change the temperature, it’ll still be a challenge for us to beat the current products.

Further Concept

This year, we have designed fusion proteins of spider toxin in conjunction with snowdrop lectin by the three-alanine linker. Through the reference searching, we found that the previous research all used the three-alanine linker. In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. So we went to the linker library in iGEM part registry website to search for a longer linker. At first, we planned to use the Flexible Peptide GS Linker(BBa_K416001) from 2010 NYU iGEM team. However, we found no result in the previous history using this linker. Therefore, we modified the sequence and added three more amino acids into the linker in case it was still too short for proper protein folding. In the experiment(See the Link), the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.(See the Link)

-->