Difference between revisions of "Team:TCFSH Taiwan/HP/Silver"

 
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           <div class="topic"><p class="text_color">The Problem & Solution</p></div>
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           <div class="topic"><p class="text_color">Professor Cheng-Ming Chang</p></div>
 
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           <div class="topic"><p class="text_color">Professor Chien-Chih Yu</p></div>
 
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          <div class="topic"><p class="text_color">Improvement of Previoius BioBrick</p></div>
 
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     <p class="title">The Problem & Solution<p>
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     <p class="title">Professor Cheng-Ming Chang<p>
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     <p class="content">Effective pest control has long been a pivotal issue in modern agriculture. With the escalating growth of world population and the change in diet, high levels of agricultural production are required to meet the demand for food production. To date, a vast majority of farmers uses chemical pesticide to kill the pest worldwide<sup>[1]</sup>, it is by far the most convenient and effective way of pesticide control. However, the increased production chemical pesticides brought entails multiple negative costs that are usually overlooked, sacrificing human health and the environment. Thus, problems of chemical pesticide involving food safety, farmers’ health, soil & water pollution, and the existence of drug-resistant insects are looming. An alternative solution of pest control is urgently needed, necessitating the nascence of our project.</p>
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     <p class="content">In order to know about the transportation process for frozen food, we had an interview with Professor Cheng-Ming Chang at National Taiwan Ocean University an expert in this field.</p>
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<img src="https://static.igem.org/mediawiki/2017/e/e3/JasonHP.jpeg" class="bigphoto" width=70%>
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    <p class="title">Overview</p>
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     <p class="content">We designed a nature-inspired system to offer farmers a safe, environmentally-friendly, accurate, and automatic style of farming. Inspired by the spiders using web and venom to achieve the predation, the system also contains two concept-resembling parts—“Device and PANTIDE.” As engineering, internet of things (IoT), and synthetic biology collide, the transference of knowledge to state-of-the-art system helps farmers no longer need to do laborious farm works and spray hazardous chemical poisons. It is a revolutionary system that not only guarantees the safety of the farmers and customers worldwide but also safeguards the sustainability of the environment and ecosystem. (see <a href="https://2016.igem.org/Team:NCTU_Formosa/Design" style="color:#44E287;">Design</a> & <a href="https://2016.igem.org/Team:NCTU_Formosa/Demonstrate" style="color:#44E287;">Device</a>)</p>
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     <p class="content">During the interview, we learned two important pieces of information. The first is that our product is not applicable for the foods that need to be preserved in the fridge. Since the highest temperature that those foods can tolerate is 15 degrees Celsius, our product, whose tolerance is 37 degrees Celsius, won’t be useful for them. The second piece of information is that the technology of temperature measuring at low temperatures is already perfect. There are both cheap and expensive products, and the accuracy also varies with the price. However, it’s good that we aim the target temperature at 37 degrees Celsius, since no one has ever attempted this temperature before. (Click <a href="https://static.igem.org/mediawiki/2017/c/cb/Changchengminginterviewnew.pdf" style="color: orange">here</a> to see more)</p>
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    <p class="title">Professor Chien-Chih Yu</p>
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    <p class="content">To know more about the transportation process of drug, we interviewed Professor Chien-Chih Yu, a professor of Pharmacy at China Medical University.</p>
  
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    <img src="https://static.igem.org/mediawiki/2017/3/3e/Professorwu.jpeg" class="bigphoto" width="70%";>
  
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    <p class="title">Improvement of Previous BioBrick</p>
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     <p class="content">The feedbacks we received are, first, there might be little application of our stickers on drugs; second, the professor suggested we aim at the R&D drugs, which are less resistant to temperature change. (See more <a href="https://static.igem.org/mediawiki/2017/0/03/Hp1tcfsh.pdf" style="color: orange">here</a>)</p>
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     <p class="content">This year, we have designed fusion proteins of spider toxin in conjunction with snowdrop lectin by the three-alanine linker. Through the reference searching, we found that the previous research all used the three-alanine linker. In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. So we went to the linker library in iGEM part registry website to search for a longer linker. At first, we planned to use the Flexible Peptide GS Linker(<a href="http://parts.igem.org/Part:BBa_K416001" style="color:#44E287;">BBa_K416001</a>) from 2010 NYU iGEM team. However, we found no result in the previous history using this linker. Therefore, we modified the sequence and added three more amino acids into the linker in case it was still too short for proper protein folding. In the experiment(See the <a href="https://2016.igem.org/Team:NCTU_Formosa/Proof#GS_Linker" style="color:#44E287;">Link</a>), the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.(See the <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030" style="color:#44E287;">Link</a>)</p>
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Latest revision as of 03:42, 2 November 2017

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JUDGING FORM

Professor Cheng-Ming Chang

In order to know about the transportation process for frozen food, we had an interview with Professor Cheng-Ming Chang at National Taiwan Ocean University an expert in this field.

During the interview, we learned two important pieces of information. The first is that our product is not applicable for the foods that need to be preserved in the fridge. Since the highest temperature that those foods can tolerate is 15 degrees Celsius, our product, whose tolerance is 37 degrees Celsius, won’t be useful for them. The second piece of information is that the technology of temperature measuring at low temperatures is already perfect. There are both cheap and expensive products, and the accuracy also varies with the price. However, it’s good that we aim the target temperature at 37 degrees Celsius, since no one has ever attempted this temperature before. (Click here to see more)

Professor Chien-Chih Yu

To know more about the transportation process of drug, we interviewed Professor Chien-Chih Yu, a professor of Pharmacy at China Medical University.

The feedbacks we received are, first, there might be little application of our stickers on drugs; second, the professor suggested we aim at the R&D drugs, which are less resistant to temperature change. (See more here)