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− | <div class="topic"><p class="text_color"> | + | <div class="topic"><p class="text_color">Vitamin</p></div> |
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<div class="splash"></div> | <div class="splash"></div> | ||
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− | <div class="topic"><p class="text_color"> | + | <div class="topic"><p class="text_color">Experiment</p></div> |
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<p class="title">Vitamin Experiment</p> | <p class="title">Vitamin Experiment</p> | ||
<img src="https://static.igem.org/mediawiki/2017/7/75/Vitamin_experiment.png" class="bigphoto" width="70%"> | <img src="https://static.igem.org/mediawiki/2017/7/75/Vitamin_experiment.png" class="bigphoto" width="70%"> | ||
− | <p class=" | + | <p class="content">After we found that UV has an impact on Vitamin, we than wanted to look further inside the relationship between UV and Vitamin. We conducted an experiment on different kinds of bottles and the degradation rate of Vitamin under sunlight. While we visit VEDAN, they told us that most of the refreshing drinks contain lots of Vitamin (especislly Vitamin B) which are easily deteriorated by UV. Generally, manufacturers put their drinks inside brown bottles to protect their product from UV. Than we sent our samples to Food Industry Research and Development Institute(Taiwan) to examine the amount of Vitamin B.</p> |
<img src="https://static.igem.org/mediawiki/2017/c/c8/Vitamin_experiment2.png" class="bigphoto" width="70%"> | <img src="https://static.igem.org/mediawiki/2017/c/c8/Vitamin_experiment2.png" class="bigphoto" width="70%"> | ||
<p class="content">However, we also found out that the percentage of Vitamin B remain dropped off rapidly to 30% and 14% in brown bottle and transparent bottle respectively. This result simply means that those refreshing drink need to be protect from UV, and this is time our sticker can show its capabilities.</p> | <p class="content">However, we also found out that the percentage of Vitamin B remain dropped off rapidly to 30% and 14% in brown bottle and transparent bottle respectively. This result simply means that those refreshing drink need to be protect from UV, and this is time our sticker can show its capabilities.</p> | ||
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<p class="content-1">Culture Setting</p> | <p class="content-1">Culture Setting</p> | ||
− | <p class="content"><br>(1) 32゚C, 200rpm, culture12hr (15ml) *continue to log phase</br><br>(2) | + | <p class="content"><br>(1) 32゚C, 200rpm, culture12hr (15ml) *continue to log phase</br><br>(2) Add (15ml) LB *re-culture</br><br>(3) Te゚C, continue cultural</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p> |
<p class="title">UV</p> | <p class="title">UV</p> | ||
<p class="content-1">Culture setting</p> | <p class="content-1">Culture setting</p> | ||
− | <p class="content"><br>(1) 37゚C, 200rpm, culture 12hr (15ml) *continue to log phase</br><br>(2) Add (15ml) LB *re-culture</br><br>(3) | + | <p class="content"><br>(1) 37゚C, 200rpm, culture 12hr (15ml) *continue to log phase</br><br>(2) Add (15ml) LB *re-culture</br><br>(3) Re-culture at 37゚C under UV</br><br>(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.</br></p> |
<p class="content-1">Expression measurement setting</p> | <p class="content-1">Expression measurement setting</p> | ||
− | <p class="content"><br>(1) Extract: Take (3ml) bacteria liquid, and centrifuge at 14000rpm, 1min</br><br>(2) | + | <p class="content"><br>(1) Extract: Take (3ml) bacteria liquid, and centrifuge at 14000rpm, 1min</br><br>(2) Add 1ml PBS, pipetting+vortex</br><br>(3) Measure the O.D. value</br></p> |
+ | |||
+ | <p class="title">Results</p> | ||
+ | <p class="content">This is the result of the biobrick K2383200 and K2383302. We culture the bacteria for 15 hours. The concentration in the figure is the concentration the bacteria. 100%= the origin liquid. We do dilution to simulate the pass of time. The minimum amount for naked eyes to recognize is 50%.</p> | ||
+ | <img src="https://static.igem.org/mediawiki/2017/6/6c/Resultstcfshnew.jpeg" class="bigphoto" width="70%"> | ||
+ | <p class="content"> </p> | ||
</div> | </div> | ||
</section> | </section> |
Latest revision as of 03:48, 2 November 2017