Team:TCFSH Taiwan/Results

HOME
TEAM
PROJECT
Description
Design
Experiments
Notebook
InterLab
Contribution
Model
Results
Demonstrate
Improve
Attributions
PARTS
Parts
Basic Parts
Composite Parts
Part Collection
SAFETY
HUMAN PRACTICES
Silver HP
Integrated and Gold
Public Engagement
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
JUDGING FORM

Experiment

Our product aims to reach a high quality of “long-term monitor”, which means considerably stable expression of color is needed. Thus, we choose chromoprotein as our reporter protein due to its steadily expression and low half-life degradation rate. (See more in here)

cj-Blue/p>

We add a LVA tag to cjBlue in order to accelerate its degrade speed. (See more in here)

Concentration simulation of cjBlue

Chromoprotein

According to our model of chromoprotein

Expression

Temperature

35ﾟC / 37ﾟC / 39ﾟC / 32ﾟC

Chromoprotein o.d. value

(1) RFP (E1010): 607nm
(2) BFP (k592009):588nm

(3) cjBlue (k592011):610nm

Culture Setting

(1) 32ﾟC, 200rpm, culture12hr (15ml) *continue to log phase
(2) add (15ml) LB *re-culture

(3) TeﾟC, continue cultural

(4) Take samples from the centrifuge tube per hour, and narrow the time range and improve the accuracy.