Difference between revisions of "Team:TU-Eindhoven/Project/Protocols"
| Line 25: | Line 25: | ||
Nanodrop<br/> | Nanodrop<br/> | ||
Agarose Gel Electrophoresis<br/> | Agarose Gel Electrophoresis<br/> | ||
| − | SDS-Page<br/><br/> | + | SDS-Page</h6><br/><br/> |
| + | |||
| + | <h1>Traditional Cloning & BioBricking</h1> | ||
| + | <h6>PCR Amplification<br/> | ||
| + | PCR Purification<br/> | ||
| + | Digestion<br/> | ||
| + | Ligation<br/> | ||
| + | LIU PCR</h6><br/><br/> | ||
| + | |||
| + | <h1>Gibson Assembly</h1> | ||
| + | <h6>Vector Linearization<br/> | ||
| + | Gibson Assembly<h6><br/><br/> | ||
| + | |||
| + | <h1>Transformation, Protein Expression & Purification</h1> | ||
| + | <h6>Transformation into NovaBlue<br/> | ||
| + | Plating<br/> | ||
| + | Colony Picking and Colony PCR<br/> | ||
| + | Sequencing<br/> | ||
| + | Miniprep<br/> | ||
| + | Small culturing<br/> | ||
| + | Protein expression<br/> | ||
| + | Protein Purification using a His-tag <br/> | ||
| + | Protein Purification using a Strep-tag</h6><br/><br/> | ||
| + | |||
| + | |||
| + | |||
Revision as of 09:04, 1 November 2017
General Protocols
General Necessities
Glycerol Stock
Streaking Glycerol Stock
Nanodrop
Agarose Gel Electrophoresis
SDS-Page
Traditional Cloning & BioBricking
PCR Amplification
PCR Purification
Digestion
Ligation
LIU PCR
Gibson Assembly
Vector Linearization
Gibson Assembly
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
Transformation, Protein Expression & Purification
Transformation into NovaBlue
Plating
Colony Picking and Colony PCR
Sequencing
Miniprep
Small culturing
Protein expression
Protein Purification using a His-tag
Protein Purification using a Strep-tag
